Evaluation of the relative available energy of cyclic nigerosylnigerose using breath hydrogen excretion in healthy humans

Abstract

Cyclic nigerosylnigerose (CNN) is a cyclic tetrasaccharide with properties distinct from those of other conventional cyclodextrins. We investigated the relative available energy of CNN in healthy humans. CNN digestibility was determined using brush border membrane vesicles from the small intestines of rats. CNN was not hydrolyzed by rat intestinal enzymes. To investigate breath hydrogen excretion, thirteen human subjects were included in a double-blind cross-over, randomized, placebo-controlled study. The effects of CNN on hydrogen excretion were compared with those of a typical nondigestible, fermentable fructooligosaccharide (FOS). In the study participants, hydrogen excretion hardly increased upon CNN and was remarkably lower than for FOS. The available energy value was determined using the fermentability based on breath hydrogen excretion and was evaluated as 0 kcal/g for CNN. CNN was hardly metabolized and hence may be used as a low-energy dietary fiber.

Creation of Novel Useful Carbohydrate Materials Using Microbial Enzymes

“…を示した (17,18) ． 3. 環状四糖水飴テトラリング ® の性質 本水飴の最大無作用量は，男性で 0.88 g/kg 体重，女 性で 0.89 g/kg 体重と判定され，下痢を起こしにくい食 物繊維素材であることが明らかになった (15) ．CNN は消 化吸収されず腸で発酵されず，エネルギー換算係数は 0 kcal/g と算出された (19) ．本水飴の成分は，消化酵素を 作用させると CNN(47％)とグルコース(53％)に分 解された．本水飴のエネルギー換算係数は CNN 以外の 53％は消化吸収されることから 2 kcal/g であると算出さ れた (17) ．本水飴摂取時の血糖値およびインスリン濃度 の上昇はグルコースよりも低かった (17) ．…”