Evaluation of the rapid antigen test Panbio COVID-19 in saliva and nasal swabs in a population-based point-of-care study

Agulló, Vanesa; Fernandez-González, Marta; Tabla, Victoria Ortiz de la; Gonzalo-Jiménez, Nieves; Masiá, Mar; Gutiérrez, Félix

doi:10.1016/j.jinf.2020.12.007

Cited by 59 publications

(65 citation statements)

References 8 publications

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“…Recent articles published in this Journal highlighted the potential of using antigen-detecting rapid diagnostic tests (Ag-RDTs) on saliva samples for massive screening of asymptomatic subjects and epidemiological surveillance [1,2]. Saliva has entered the shortlist of clinical samples to which the current laboratory tests can be applied, due to increasing evidences of comparable sensitivity and specificity with respect to nasopharyngeal swabs (NPS) [3][4][5][6].…”

Section: Dear Editormentioning

confidence: 99%

“…However, this did not represent a major obstacle to achieve same day diagnostic definition, due to the timely organization and information flow. On the other hand, the disadvantage of longer process was overcome by the greater accuracy of a laboratory test compared to a POCT [2,9,10] and the ability to quickly perform the confirmation test with a system compatible with "urgent" execution.…”

mentioning

confidence: 99%

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Effective screening strategy against SARS-CoV-2 on self-collected saliva samples in primary school setting: A pilot project

Bordi

Parisi

Sberna

et al. 2021

Journal of Infection

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Dear Editormentioning

confidence: 99%

mentioning

confidence: 99%

Effective screening strategy against SARS-CoV-2 on self-collected saliva samples in primary school setting: A pilot project

Bordi

Parisi

Sberna

et al. 2021

Journal of Infection

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show abstract

“…It is similar to sampling traditionally performed for PCR tests, and is in agreement with the conclusions of a recent study enrolling 659 patients that employed the Panbio test and showed excellent sensitivity for the combination of saliva and nasal sampling. 21 When combined with the 350 µL of extraction buffer, available from the test kit, the 100 µL extract required for the lateral flow assay was obtained without the need for any additional fluid. When the team test was performed eight times, we obtained a signal intensity of 43 ± 6 % (mean ± one SD) of that for the single swab standard procedure (entry No.…”

Section: Resultsmentioning

confidence: 99%

Reducing the Cost of Rapid Antigen Tests through Swab Pooling and Extraction in a Device

Berking

Ulrich

et al. 2021

Preprint

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The COVID-19 pandemic places a significant stress on the viral testing capabilities of many countries. The value of rapid point-of-care (PoC) antigen tests is becoming increasingly clear, but implementing frequent large scale testing is costly. We have developed an inexpensive device for pooling swabs, extracting specimens, and detecting viral antigens with a commercial lateral flow assay detecting the nucleocapsid protein of SARS-CoV-2 as antigen. The holder of the device can be produced locally through 3D printing. The extraction and the elution can be performed with the entire set-up encapsulated in a transparent bag, minimizing the risk of infection for the operator. With 6 swabs holding approx. 0.1 mL specimen each and 0.35 mL extraction buffer, 43+/-6 % (n= 8) of the signal for an individual extraction of a positive control standard was obtained. Image analysis still showed a signal-to-noise ratio of > 7 upon further eight-fold dilution. Our current total cost of materials is below $ 2 per tested person or 20% of our cost for an individual PoC test. These findings suggest that pooling can make frequent testing more affordable for schools, universities and other institutions, without decreasing sensitivity to an unacceptable level. Further validation of the method is required.

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“…While lower values of cycle threshold seem to link with detecting culturable virus and higher chances of generating secondary cases, 48‐51 careful interpretations of oversimplified modeling assumptions are warranted. Scant independent evaluations from real‐world data, several case reports that present lower sensitivity especially among asymptomatic cases, questionable workability of home‐based approaches because of differences in sampling methods, procurement and deployment of these tools, logistics for registering the data, and negative impacts of rapid testing results on modifying people's behaviors including false sense of security among citizens, breaking chains of COVID‐19, and protecting asymptomatic and presymptomatic COVID‐19 patients before their symptoms of onset will be eminent challenges 52‐56 . Discussing the feasibility in each context, summarizing advantages and disadvantages of testing tools for public health purposes, and intervals of mass testing will be vital research topics during COVID‐19 pandemic.…”

Section: Discussionmentioning

confidence: 99%

Modeling population‐wide testing of SARS‐CoV‐2 for containing COVID‐19 pandemic in Okinawa, Japan

Shimizu

Kuniya

Tokuda

2021

J of Gen and Family Med

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To break the chains of SARS‐CoV‐2 transmission and contain the coronavirus disease 2019 (COVID‐19) pandemic, population‐wide testing has been practiced in various countries. However, scant research has addressed this topic in Japan. In this modeling exercise, we extracted the number of daily reported cases of COVID‐19 in Okinawa from October 1 to November 30, 2020, and explored possible scenarios for decreasing COVID‐19 incidence by combining population‐wide screening and/or social distancing policy. We reveal that permanent lockdown can be theoretically replaced by mass testing but sufficient target population at an adequate frequency must be mobilized. In addition, solely imposing a circuit breaker will not bring a favorable outcome in the long run, and mass testing presents implications for minimizing a period of lockdown. Our results highlight the importance of incentivizing citizens to join the frequent testing and ensure their appropriate isolation. This study also suggests that early containment of COVID‐19 will be feasible in prefectures where the mobility is low and/or can be easily controlled for its geographic characteristics. Rigorous investment in public health will be manifestly vital to contain COVID‐19.

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Evaluation of the rapid antigen test Panbio COVID-19 in saliva and nasal swabs in a population-based point-of-care study

Cited by 59 publications

References 8 publications

Effective screening strategy against SARS-CoV-2 on self-collected saliva samples in primary school setting: A pilot project

Effective screening strategy against SARS-CoV-2 on self-collected saliva samples in primary school setting: A pilot project

Reducing the Cost of Rapid Antigen Tests through Swab Pooling and Extraction in a Device

Modeling population‐wide testing of SARS‐CoV‐2 for containing COVID‐19 pandemic in Okinawa, Japan

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