Evaluation of the radiosensitizing potency of bromelain for radiation therapy of 4T1 breast cancer cells

Abstract

Breast cancer (BC) remains the leading cause of death in women worldwide, despite the improvements of cancer screening and treatment methods. Recently, development of novel anticancer drugs for the improved prevention and treatment of BC is in the center of research. The anticancer effects of bromelain, as enzyme extract derived from the pineapples, contains chemicals that interfere with the growth of tumor cells. The aim of this study was to evaluate the effect of radiosensitizing of bromelain in 4T1 BC cells. This investigation utilized the 3-(4,5-dimethylthiazol-2-yl)-2,5-dimethyltetrazolium bromide assay to characterize the cytotoxicity of bromelain. Colony formation method was used to establish the truth of the capability of bromelain to make sensitive to radiation therapy. Flowcytometry performed to define the contribution the apoptosis effect to bromelain mediated radiosensitization of 4T1 cells. Bromelain reduced growth and proliferation of 4T1 cell as a concentration-dependence manner significantly. The survival of 4T1 cancer cells was decreased after combined treatment in a number and size-dependent manner with regard to the control group (P < 0.05). Combination of bromelain with radiation does not influence 4T1 cell apoptosis. The results suggested that bromelain can inhibit the growth and proliferation and reduce survival of 4T1 BC cells and might be used as a candidate radiosensitizer in BC patient.

Does Bromelain-Cisplatin Combination Afford In-Vitro Synergistic Anticancer Effects on Human Prostatic Carcinoma Cell Line, PC3?

“…The aptitude of bromelain to engage in-vitro human leukemia cell differentiation was already mechanisms reported. Additionnaly, bromelain proved to be of radiosensitizing feature in 4T1 mouse breast cancer cell line [ 14 ]. In the current study, single-agent bromelain or cisplatin treatment displayed a significant dose and time-dependent effect on inhibition of PC3 cell proliferation at three different incubation times of 24, 48 and 72 hours ( Figure-1 ).…”

“…Cell culture efficiency was measured by the formula [yield efficiency=(number of colonies formed / number of cultured cells) × 100]. The survival ratio for treatment groups was calculated using the formula [survival ratio=(efficiency of cultures with treatment / efficiency of cultures without treatment) × 100) [ 14 ].…”

Radiosensitizing Effect of Bromelain Using Tumor Mice Model via Ki-67 and PARP-1 Inhibition

“…The radioresistance of MCF-7 breast carcinoma cells was mention before in the study of Jänicke et al 43 Previous reports showed that the in vitro treatment of tumor cell lines with BL results in inhibition of cell growth and invasion capacities. 26 , 44 , 45 It has been reported that BL has a tumoricidal activity in vitro via induction of ROS and subsequent mitochondrial membrane depolarization, later on leading to oxidative stress as well as through its autophagy action. 46 , 47 The anticancer effect of BL has been credited mainly to its protease component through digestion and diffusion in tumor cells.…”

Does Bromelain-Cisplatin Combination Afford In-Vitro Synergistic Anticancer Effects on Human Prostatic Carcinoma Cell Line, PC3?

“…Cell culture efficiency was measured by the formula [yield efficien-cy=(number of colonies formed / number of cultured cells) × 100]. The survival ratio for treatment groups was calculated using the formula [survival ratio=(efficiency of cultures with treatment / efficiency of cultures without treatment) × 100) [14].…”