Evaluation of the Protective Effects of Sarains on H2O2-Induced Mitochondrial Dysfunction and Oxidative Stress in SH-SY5Y Neuroblastoma Cells

Alvariño, Rebeca; Albrecht, Eva; Tribalat, Marie-Aude; Gegunde, Sandra; Thomas, Olivier P.; Botana, Luís M.

doi:10.1007/s12640-017-9748-3

Cited by 23 publications

(22 citation statements)

References 60 publications

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“…It has been reported that oxidative stress plays a crucial role in PD (Jenner, 2003;Puspita et al, 2017). Some natural products were reported to prevent oxidative stress in H 2 O 2 -treated SH-SY5Y cells (Alvarino et al, 2017;De Oliveira et al, 2017;De Oliveira et al, 2018). However, the effects of a-cyperone in H 2 O 2 -treated SH-SY5Y cells have not been explored.…”

Section: Introductionmentioning

confidence: 99%

α-Cyperone Attenuates H2O2-Induced Oxidative Stress and Apoptosis in SH-SY5Y Cells via Activation of Nrf2

Huang

Liu

et al. 2020

Front. Pharmacol.

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a-Cyperone, extracted from Cyperus rotundus, has been reported to inhibit microgliamediated neuroinflammation. Oxidative stress and apoptosis play crucial roles in the course of Parkinson's disease (PD). PD is a common neurodegenerative disease characterized by selective death of dopaminergic neurons. This study was designed to investigate the neuroprotective effects of a-cyperone against hydrogen peroxide (H 2 O 2)induced oxidative stress and apoptosis in dopaminergic neuronal SH-SY5Y cells. Neurotoxicity was assessed by MTT assay and the measurement of lactic dehydrogenase (LDH) release. The level of reactive oxygen species (ROS) was measured by dichlorodihydrofluorescin diacetate (DCFH-DA) staining. The apoptosis of SH-SY5Y cells was evaluated by annexin-V-FITC staining. The translocation of NF-E2related factor 2 (Nrf2) was determined by western blot and immunofluorescence staining. Western blot analysis was conducted to determine the expression level of cleavedcaspase-3, the pro-apoptotic factor Bax, and the anti-apoptotic factor, Bcl-2. The results showed that a-cyperone substantially decreased H 2 O 2-induced death, release of LDH, and the production of ROS in SH-SY5Y cells. In addition, we found that a-cyperone attenuated H 2 O 2-induced cellular apoptosis. Moreover, a-cyperone remarkably reduced the expression of cleaved-caspase-3 and Bax, and upregulated Bcl-2. Furthermore, acyperone enhanced the nuclear translocation of Nrf2. Pretreatment with brusatol (BT, an Nrf2 inhibitor) attenuated a-cyperone-mediated suppression of ROS, cleaved-caspase-3, and Bax, as well as a-cyperone-induced Bcl-2 upregulation in H 2 O 2-treated SH-SY5Y cells. a-cyperone neuroprotection required Nrf2 activation. In conclusion, a-cyperone attenuated H 2 O 2-induced oxidative stress and apoptosis in SH-SY5Y cells via the activation of Nrf2, suggesting the potential of this compound in the prevention and treatment of PD.

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Section: Introductionmentioning

confidence: 99%

α-Cyperone Attenuates H2O2-Induced Oxidative Stress and Apoptosis in SH-SY5Y Cells via Activation of Nrf2

Huang

Liu

et al. 2020

Front. Pharmacol.

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“…The effect of laxaphycins on cell metabolic activity was evaluated by MTT (3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) assay [27,49]. SH-SY5Y cells were cultured in 96-well plates and treated with compounds at concentrations ranging from 0.001 to 10 µM for 24 h. Next, cells were rinsed three times and incubated for 1 h with MTT (500 µg/mL) dissolved in saline buffer.…”

Section: Metabolic Activity Evaluationmentioning

confidence: 99%

“…∆Ψ m was evaluated with TMRM assay as previously described [49]. SH-SY5Y cells were seeded in 96-well plates at 5 × 10 4 cells per well.…”

Section: Mitochondrial Membrane Potential Measurementmentioning

confidence: 99%

“…ROS production was assessed with the fluorescence dye carboxy-H 2 DCFDA (5-(and-6)-carboxy-2 ,7 -dichlorodihydrofluorescein diacetate) [49]. SH-SY5Y cells were seeded in 96-well plates and allowed to grow for 24 h. Then, cells were treated with laxaphycins (0.001-10 µM) for 24 h and ROS levels were measured.…”

Section: Evaluation Of Reactive Oxygen Species and Atp Levelsmentioning

confidence: 99%

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Biological Activities of Cyclic and Acyclic B-Type Laxaphycins in SH-SY5Y Human Neuroblastoma Cells

et al. 2020

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Laxaphycins are a family of non-ribosomal lipopeptides that have been isolated from several cyanobacteria. Some of these compounds have presented cytotoxic activities, but their mechanism of action is poorly understood. In this work, the already described laxaphycins B and B3, and acyclolaxaphycins B and B3 were isolated from the marine cyanobacteria Anabaena torulosa. Moreover, two new acyclic compounds, [des-(Ala4-Hle5)] acyclolaxaphycins B and B3, were purified from the herviborous gastropod Stylocheilus striatus, with this being the first description of biotransformed laxaphycins. The structure of these new compounds was elucidated, together with the absolute configuration of acyclolaxaphycins B and B3. The bioactivities of the six peptides were determined in SH-SY5Y human neuroblastoma cells. Laxaphycins B and B3 were cytotoxic (IC50: 1.8 and 0.8 µM, respectively) through the induction of apoptosis. In comparison, acyclic laxaphycins did not show cytotoxicity but affected mitochondrial functioning, so their effect on autophagy-related protein expression was analyzed, finding that acyclic peptides affected this process by increasing AMPK phosphorylation and inhibiting mTOR. This work confirms the pro-apoptotic properties of cyclic laxaphycins B and is the first report indicating the effects on autophagy of their acyclic analogs. Moreover, gastropod-derived compounds presented ring opening and amino-acids deletion, a biotransformation that had not been previously described.

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“…H2O2-induced oxidative stress reduced glutathione level [25], induced DNA damage and apoptosis in cultured cells [26]. H2O2 action is accompanied by mitochondrial depolarization, aggregation, and cytochrome C release [27], and increased MAPK and p38 [28] in other cells and SH-SY5Y cells [29].…”

Section: Oxyresveratrol Content In Puag-haadmentioning

confidence: 99%

Neuroprotective Effect of Artocarpus Lakoocha Extract and Oxyresveratrol Against Hydrogen Peroxide-Induced Toxicity in Sh-Sy5y Cells

Hasriadi

Wongon

Lapphanichayakool

et al. 2017

Int J Pharm Pharm Sci

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Objective: Artocarpuslakoocha Roxb. Is a traditional medical plant native to Southeast Asia and used as a dried aqueous extract so-called puaghaad. Its role (and its major ingredient, oxyresveratrol) as an antioxidant neuroprotectant were explored.Methods: Differentiated SH-SY5Y neuroblastoma cells in 96-well plates were challenged with 200 µM H2O2 for 4h and either Trolox (100 µM), oxyresveratrol (5-100 µM), or puaghaad (1.2-25 µg/ml) applied 2h before H2O2 or for 20 h after H2O2Results: Continuous presence of both H washout. Cell viability, mitochondrial function, intracellular ROS, and lipid peroxidation were assessed.2O 2 and antioxidant reduced mitochondrial function by ~50% but only by 30% with antioxidant. Sustained 24 h H2O2 showed no recoveries with antioxidants. Cell viability was modestly restored when antioxidants accompanied H2O2 for 4 h and both washed for another 20 h, but little recovery of mitochondrial function even though antioxidants removed ROS and prevent lipid peroxidation. Antioxidants added for 20 h after H2O2 Conclusion:These results show that mitochondrial protection was illusive, yet both tested compounds, puaghaad and oxyresveratrol, improved cell viability and especially ROS levels and lipid peroxidation. The potency oxyresveratrol on theredox-sensitive expression of antioxidant enzymes and its pharmacokinetics suggests that oral puaghaad could provide effective protection in transient neurodegenerative disease. marginally improve mitochondria and modestly restore cell viability, but lipid peroxidation was completely reversed.

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Evaluation of the Protective Effects of Sarains on H2O2-Induced Mitochondrial Dysfunction and Oxidative Stress in SH-SY5Y Neuroblastoma Cells

Cited by 23 publications

References 60 publications

α-Cyperone Attenuates H2O2-Induced Oxidative Stress and Apoptosis in SH-SY5Y Cells via Activation of Nrf2

α-Cyperone Attenuates H2O2-Induced Oxidative Stress and Apoptosis in SH-SY5Y Cells via Activation of Nrf2

Biological Activities of Cyclic and Acyclic B-Type Laxaphycins in SH-SY5Y Human Neuroblastoma Cells

Neuroprotective Effect of Artocarpus Lakoocha Extract and Oxyresveratrol Against Hydrogen Peroxide-Induced Toxicity in Sh-Sy5y Cells

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