Evaluation of the protective effect on exogenous melatonin in adult rats and their offspring exposed to insecticides methomyl and cypermethrin during pregnancy

Marinho, Ketsia Sabrina do Nascimento; Neto, Clóvis José Cavalcanti Lapa; Coelho, Ilka Dayane Duarte de Sousa; Silva, Meykson Alexandre da; Melo, Maria Eduarda Gomes; Santos, Katharine Raquel Pereira dos; Chagas, Cristiano Aparecido; Teixeira, Álvaro Aguiar Coelho; Teixeira, Valéria Wanderley

doi:10.1016/j.mrgentox.2019.503107

Cited by 12 publications

(7 citation statements)

References 33 publications

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“…Methomyl‐, carbaryl‐, metalaxyl‐, and pendimethalin‐treated (1000 μM) HUVECs showed 24.33‐, 28.72‐, 32.44‐, and 28.11‐fold greater OTM values, as compared with the untreated and DMSO 0.1% controls, which showed OTM values of 0.987 and 0.854, respectively. The comet assay data are in accordance with the previous reports confirming the DNA damaging ability in dams of pregnant rats exposed to methomyl (0.02 mg/kg/day) (do Nascimento Marinho et al, 2019); human lymphocytes treated with 200 μM of pendimethalin (Ansari et al, 2018); DNA damage in human sperm environmentally exposed to carbaryl (Meeker et al, 2004); and chromosomal DNA damage in metalaxyl‐treated human lymphocytes (Demsia et al, 2007). It has been implicated that oxidative stress in mitochondria can inflict oxidative stress on nucleus, thereby introducing DNA strand breaks (Nadalutti et al, 2020).…”

Section: Discussionsupporting

confidence: 92%

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Cytotoxicity and genotoxicity of methomyl, carbaryl, metalaxyl, and pendimethalin in human umbilical vein endothelial cells

Saquib

Siddiqui

Ansari

et al. 2021

J of Applied Toxicology

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Pesticides have adverse effects on the cellular functionality, which may trigger myriad of health consequences. However, pesticides‐mediated toxicity in the endothelial cells (ECs) is still elusive. Hence, in this study, we have used human umbilical vein endothelial cells (HUVECs) as a model to quantify the cytotoxicity and genotoxicity of four pesticides (methomyl, carbaryl, metalaxyl, and pendimethalin). In the MTT assay, HUVECs exposed to methomyl, carbaryl, metalaxyl, and pendimethalin demonstrated significant proliferation inhibition only at higher concentrations (500 and 1000 μM). Likewise, neutral red uptake (NRU) assay also showed proliferation inhibition of HUVECs at 500 and 1000 μM by the four pesticides, confirming lysosomal fragility. HUVECs exposed to the four pesticides significantly increased the level of intracellular reactive oxygen species (ROS). Comet assay and flow cytometric data exhibited DNA damage and apoptotic cell death in HUVECs after 24 h of exposure with methomyl, metalaxyl, carbaryl, and pendimethalin. This is a first study on HUVECs signifying the cytotoxic–genotoxic and apoptotic potential of carbamate insecticides (methomyl and carbaryl), fungicide (metalaxyl), and herbicide (pendimethalin). Overall, these pesticides may affect ECs functions and angiogenesis; nonetheless, mechanistic studies are warranted from the perspective of vascular biology using in vivo test models.

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Section: Discussionsupporting

confidence: 92%

“…CD‐1 mice exposed to methomyl showed reduction in their antioxidant enzymes level (El‐Demerdash et al, 2013). Furthermore, methomyl demonstrated its high toxicity after its conversion into acetonitrile then to cyanide by the liver microsomal detoxification systems (do Nascimento Marinho et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and genotoxicity of methomyl, carbaryl, metalaxyl, and pendimethalin in human umbilical vein endothelial cells

Saquib

Siddiqui

Ansari

et al. 2021

J of Applied Toxicology

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“…Similarly, CP has also been shown to cause genotoxic effects through DNA damage and cell apoptosis 40,44 . The improvement in live/dead ratio following pretreatment or posttreatment with ML suggests that the antioxidant agent counteracted the pesticide-induced toxicity possibly by protecting against ROS-induced DNA damage and protein oxidation 45 coupled with its ability to decrease the level of certain pro-apoptotic enzymes like caspases 3 and 9 46 and its ability to protects against genotoxic damages 47 , which eventually reduced prenatal and neonatal mortality.…”

Section: Discussionmentioning

confidence: 99%

Melatonin Ameliorates Developmental Landmarks Induced by Gestational and Lactational Coexposure to Chlorpyriphos and Cypermethrin in F1 Male Rats

Shittu

Ambali

Ayo

et al. 2021

Preprint

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The ameliorative potentials of melatonin (ML) on developmental changes evoked by gestational and lactational co-exposure to chlorpyriphos (CP) and cypermethrin (CY) was investigated in male Wistar rats. Pregnant dams were divided at random into 6 groups of 10 animals each and treated orally by gavage from gestation day 1 to postnatal day 21 with the following regimens: The DW, SO and ML groups were administered distilled water (2 ml/kg), soya oil (2 ml/kg) and melatonin (0.5 mg/kg), respectively; CC group was co-administered CP (1.9 mg/kg) and CY (7.5 mg/kg); MC group was pretreated with ML (0.5 mg/kg) and followed by co-administration of CP and CY while the CM group was administered CP and CY and then treated with ML. We evaluated the developmental parameters on the F1 generation male rats at different postnatal intervals following parturition. Alterations in litter size and weight, number of live/dead pups, anogenital distance, crown-rump length, time of eye and ear openings, and testicular descent induced by gestational and lactational exposure to CP and CY in F1 male rats were mitigated by pre- and post-administration of ML. These curative and prophylactic potentials of ML may be partly attributed to its widely known antioxidant property.

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“…Bhatti, Sidhu e Bhatti (2011) confirmaram o efeito protetor de 10 mg/kg de melatonina exógena contra o estresse oxidativo induzido pela administração intraperitoneal de Atrazina (300 mg/kg) em eritrócitos de ratos, por um período de 21 dias. No trabalho de Marinho et al (2019) a melatonina aplicada durante a gestação na dosagem de 10mg/kg diminuiu os efeitos genotóxicos da cipermetrina, mas não os do metomil. Pelo menos para a cipermetrina, a melatonina pode ser eficaz na proteção das células não apenas dos efeitos genotóxicos, mas também de outros efeitos associados à produção de radicais livres.…”

Section: Efeito Protetor Da Melatonina Contra Os Agrotóxicosunclassified

Tratamento Sustentável De Resíduos Sólidos Orgânicos De Diferentes Origens Pelo Processo De Compostagem Em Larga Escala

Parajara¹,

Barbosa²

2021

Desenvolvimento Sustentável, Interdisciplinaridade E Ciências Ambientais 2

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Direitos para esta edição cedidos à Atena Editora pelos autores. Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição-Não-Comercial-NãoDerivativos 4.0 Internacional (CC BY-NC-ND 4.0).O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, inclusive não representam necessariamente a posição oficial da Atena Editora. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais. Todos os manuscritos foram previamente submetidos à avaliação cega pelos pares, membros do Conselho Editorial desta Editora, tendo sido aprovados para a publicação com base em critérios de neutralidade e imparcialidade acadêmica.A Atena Editora é comprometida em garantir a integridade editorial em todas as etapas do processo de publicação, evitando plágio, dados ou resultados fraudulentos e impedindo que interesses financeiros comprometam os padrões éticos da publicação. Situações suspeitas de má conduta científica serão investigadas sob o mais alto padrão de rigor acadêmico e ético.

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Evaluation of the protective effect on exogenous melatonin in adult rats and their offspring exposed to insecticides methomyl and cypermethrin during pregnancy

Cited by 12 publications

References 33 publications

Cytotoxicity and genotoxicity of methomyl, carbaryl, metalaxyl, and pendimethalin in human umbilical vein endothelial cells

Cytotoxicity and genotoxicity of methomyl, carbaryl, metalaxyl, and pendimethalin in human umbilical vein endothelial cells

Melatonin Ameliorates Developmental Landmarks Induced by Gestational and Lactational Coexposure to Chlorpyriphos and Cypermethrin in F1 Male Rats

Tratamento Sustentável De Resíduos Sólidos Orgânicos De Diferentes Origens Pelo Processo De Compostagem Em Larga Escala

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