Evaluation of the Properties of<i>Gongronema latifolium</i>in Phospholipon 90H Based Solid Lipid Microparticles (SLMs): An Antidiabetic Study

Chime, Salome Amarachi; Onyishi, Ikechukwu V.; Ugwoke, Paschal U.; Attama, Anthony A.

doi:10.3109/19390211.2013.859212

Cited by 12 publications

(12 citation statements)

References 18 publications

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“…In this study, we demonstrated the inhibitory actions of four pregnane phytochemicals alongside their source chromatographic fractions derived from GLB against α-amylase, α-glucosidase and sucrase in vitro. The observed inhibitory potential of the pregnane-rich chromatographic fractions derived from GLB provides mechanistic insights into the in vivo anti-hyperglycaemic and anti-diabetic activities of various extracts from GLB extensively reported for over a decade [18][19][20]28].…”

Section: Discussionmentioning

confidence: 99%

“…Besides, the leaves are incorporated into a tea blend that is mainly marketed to diabetes mellitus patients in the United States [16,17]. Several in vivo studies have helped to provide support for the ethnopharmacological use of GLB against hyperglycaemia, obesity, and diabetes [18][19][20]. For instance, methanol extracts alone and in combination with glibenclamide decreased the blood glucose from 1 to 6 h post-treatment in alloxan-induced hyperglycaemic rats [21].…”

Section: Introductionmentioning

confidence: 99%

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Marsectohexol and other pregnane phytochemicals derived from Gongronema latifolium as α-amylase and α-glucosidase inhibitors: in vitro and molecular docking studies

et al. 2020

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This study employed in vitro and in silico techniques to screen isolated pregnane phytochemicals (P1-P4), as well as their source pregnane-rich chromatographic fractions, which were derived from Gongronema latifolium Benth (GLB), for their inhibitory potentials against α-amylase, α-glucosidase, and sucrase enzyme activities. The results show that, most pregnane-rich chromatographic fractions and isolated compounds exhibited good inhibitory activities against α-amylase, and α-glucosidase enzymes. Among the isolated compounds, marsectohexol (P2) is the most favourable structure that showed inhibition against α-amylase (IC 50 = 3.712 µg/mL) when compared with the reference inhibitor, acarbose ((IC 50 = 15.418 µg/mL). Based on negative and low value of ∆G (Gibb's free energy), docking scores revealed that, marsectohexol has the highest binding affinity (− 8.8 kcal/mol) to human pancreatic α-amylase (HPA) as compared to the standard acarbose (− 8.1 kcal/mol). Marsectohexol and the other phytocompounds were also found to dock into the active site of HPA; interacting with key catalytic amino acid residues (ASP197, GLU233 and ASP300), which are known to involve in the nucleophilic, and the acid/base catalysis of the enzyme. Although, the pregnane phytocompounds have considerable high affinity binding to the human lysosomal α-glucosidase, they interacted with pockets other than the active site. In conclusion, at least, marsectohexol and three other constituent pregnane phytochemicals of GLB are potential inhibitors of the carbohydrate-hydrolyzing enzymes. These compounds may account for the anti-hyperglyceamic and antidiabetic potentials of this food herb.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Marsectohexol and other pregnane phytochemicals derived from Gongronema latifolium as α-amylase and α-glucosidase inhibitors: in vitro and molecular docking studies

et al. 2020

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“…The WHO estimates that 70–80% of the people in developing countries use traditional medicine as a major source of health care [25]. GL is a herb that has long been an integral part of African traditional medicine and its beneficial properties have been well documented, especially as an antidiabetic herb [1, 9, 11, 26, 27]. In this study, we investigated an ethanolic extract of GL because similar extracts have been reported to be the most efficacious in exerting the anti-diabetic effect [28].…”

Section: Discussionmentioning

confidence: 99%

“…Several pharmacological activities of GL extracts have been studied and reported, which provided experimental support for the empirical ethnopharmacological use of this plant in folk medicine. For example, anti-inflammatory [6], antifungal [7], anti-laxative [8] and antidiabetic [9, 10] activities have been reported. Over the past two decades, different parts of GL have been found to contain saponins, anthraquinones, alkaloids, β-sistosterol, sitostenone, lupenyl esters, pregnance ester, glucosides, and essential oils [10–12].…”

Section: Introductionmentioning

confidence: 99%

Safety assessment of the ethanolic extract of Gongronema latifolium Benth. leaves: a 90-day oral toxicity study in Sprague Dawley rats

Al-Hindi

Yusoff

Ahmad

et al. 2019

BMC Complement Altern Med

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Background The leaves of Gongronema latifolium Benth. have long been recognized traditionally as a remedy for a variety of ailments in Africa. This study was conducted to evaluate the safety profile of the ethanolic extract of G. latifolium (GLES) leaves through a repeated dose 90-day oral toxicity study in male and female of Sprague Dawley rats. Methods GLES was orally administered at doses of 250, 500 and 1000 mg/kg/day consecutively for 90 days. Results No behavioral or physiological changes and mortality were observed. GLES did not have a marked impact on general hematological parameters and did not precipitate nephrotoxicity. However, compared to the control, serum triglycerides, total cholesterol and low-density lipoprotein levels were lower and white adipose tissue paired retroperitoneal fat depots were depleted in male rats treated with GLES3 by the end of the experiment. The liver was significantly enlarged in GLES-treated rats of both sexes. Negative gender-specific alterations were observed with the highest dose. Adverse risk was evident in the female rats mainly due to marked body weight gain and cerebrum weight reduction. Conclusion Further research is needed to reach more specific conclusions about to the safety of ingesting high doses of GLES for long periods of time.

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“…16,17 Aqueous and ethanolic leaf extracts of the plant have been reported to increase the frequency of spontaneously contracting tissues and directly stimulate uterine contractions in albino rats. 18 Similarly, ethanolic extracts of the leaves and stem of N. laevis have been reported to possess antioxidant activity in diabetic rats. 19 Since the fundamental importance of using plant-based medicines is their relative safety compared with synthetic drugs, and affordability of treatment, the objectives of this study were to formulate SLMs encapsulating N. laevis leaves extract and to study its analgesic/anti-nociceptive property.…”

Section: Introductionmentioning

confidence: 99%

Development, in vitro and in vivo evaluations of novel lipid drug delivery system of Newbouldia laevis (P. Beauv.)

et al. 2016

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Newbouldia laevis (P. Beauv.) is a tropical rainforest plant used in traditional folk medicine for the treatment of malaria, cough, joint pains, stomach ache, oedema and inflammation. The main thrust of this research work was to study the analgesic/anti-nociceptive properties of N. laevis-loaded solid lipid microdispersions. N. laevis leaves were extracted using ethanol, and the extract was formulated into solid lipid microdispersions using lipid matrix comprising a rational blend of Precirol® ATO 5 and Softisan® 154. Characterization of the solid lipid microdispersions include determination of morphology, particle size, pH, thermal property, encapsulation efficiency percentage and analgesic/anti-nociceptive property. The results obtained showed that the particles were spherical with sizes ranging from 40 µm to 125 µm. The solid lipid microdispersions maintained a stable pH within the acidic region of 5–6 with insignificant variations (p > 0.05) over a period of 90 days. Thermal analysis showed that N. laevis was entrapped in the lipid matrix used for the formulations. Solid lipid microdispersions recorded a maximum encapsulation efficiency up to 88.1%. N. laevis-loaded solid lipid microdispersions also produced good analgesic/anti-nociceptive property comparable with the standard diclofenac potassium. N. laevis-loaded solid lipid microdispersions showed good analgesic/anti-nociceptive effect and could be used in the treatment and management of pain.

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Evaluation of the Properties ofGongronema latifoliumin Phospholipon 90H Based Solid Lipid Microparticles (SLMs): An Antidiabetic Study

Abstract: Gongronema latifolium-loaded SLMs exhibited a good hypoglycemic effect and could be used for the treatment of diabetes.

Cited by 12 publications

References 18 publications

Marsectohexol and other pregnane phytochemicals derived from Gongronema latifolium as α-amylase and α-glucosidase inhibitors: in vitro and molecular docking studies

Marsectohexol and other pregnane phytochemicals derived from Gongronema latifolium as α-amylase and α-glucosidase inhibitors: in vitro and molecular docking studies

Safety assessment of the ethanolic extract of Gongronema latifolium Benth. leaves: a 90-day oral toxicity study in Sprague Dawley rats

Development, in vitro and in vivo evaluations of novel lipid drug delivery system of Newbouldia laevis (P. Beauv.)

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