Evaluation of the probiotic and functional potential of Lactobacillus agilis 32 isolated from pig manure

Abstract

Escherichia coli is a symbiotic bacterium in humans and animals and an important pathogen of humans and animals. Prevention and suppression of E. coli infection is of great concern. In this study, we isolated a strain of Lactobacillus agilis 32 from pig manure and evaluated its biological characteristics, and found that its bacterial survival rate was 25% after 4 h of treatment at pH 2, and under the condition of 0·5% bile concentration, its survival rate exceeds 30%. In addition, L. agilis 32 has a cell surface hydrophobicity of 77·8%, and exhibits 67·1% auto‐aggregation and 63·2% aggregation with Enterotoxigenic E. coli 10 (ETEC 10). FITC fluorescence labelling showed that the fluorescence intensity of cecum was significantly higher than that of duodenum, jejunum or colon (P < 0·05), but no significant difference from ileum. Lactobacillus agilis 32 bacterial culture and CFS showed average inhibition zone diameters of 14·2 and 15·4 mm respectively. Lactobacillus agilis 32 CFS treatment can significantly reduce the pathogenicity of ETEC 10. These results show that L. agilis 32 is an active and potential probiotic, and it has a good antibacterial effect on ETEC10, which provides basic research for probiotics to prevent and treat intestinal diarrhoea pathogen infection.

Lactobacillus salivarius Promotion of Intestinal Stem Cell Activity in Hens Is Associated with Succinate-Induced Mitochondrial Energy Metabolism

“…In our study, the strong bacteriostatic activity of L. salivarius supernatant and L. agilis supernatant were verified. In agreement, L. salivarius and L. agilis 32 were previously proved to present an excellent antibacterial effect on pathogenic bacterium of humans or chickens ( 25 , 30 ). These implied that the stimulation from gut flora to ISCs was alleviated after feeding L. salivarius or L. agilis .…”

“…Concerning L. salivarius or L. agilis , Xia et al ( 24 ) pointed out that the colonization of L. salivarius LI01 in rats would produce indole-3-lactic acid and enhance arginine metabolism in the intestine, exerting an effect on energy accumulation in the liver. Shi et al ( 25 ) suggested that L. agilis 32 was tolerant to the gastrointestinal environment of chicken and processed an excellent antibacterial effect on E. coli 10, primarily related to its metabolites or low pH conditions. Recently, intestinal microbes derived succinate was demonstrated to induce tuft cell expansion by increasing the TCA cycle (tricarboxylic acid cycle), thus further reducing inflammation in mice’s Crohn’s-like ileitis ( 26 ).…”

16S-rRNA-Based Metagenomic Profiling of the Bacterial Communities in Traditional Bulgarian Sourdoughs

“…Along the grain-feed chain, a strain of Lig . agilis was isolated from pig manure and showed promising probiotic potential [ 76 ].…”

Baicalin–Zinc Complex Alleviates Inflammatory Responses and Hormone Profiles by Microbiome in Deoxynivalenol Induced Piglets

“…Moreover, our results showed that there was a significant difference in beta diversity between DON and DBZN groups, which means that dietary BZN in DON-contaminated diets changed the intestinal microbiota structure of piglet. Heatmaps showed that Lactobacillus ruminis and Lactobacillus agilis were enriched in the DBZN group, and they were able to inhibit Escherichia coli, which means BZN may treat intestinal pathogen infection (50,51). PICRUST showed that the dietary BZN supplementation in DON-contaminated diet enriched peptidases pathway and dietary BZN supplementation in basal diet-enriched secretion system and bacterial mobility protein pathway.…”