Evaluation of the possible protective role of vitamin A on methotrexate-induced changes on the jejunal mucosa of adult male albino rat: Histological and immunohistochemical study

El-Bakary, Nafisa A.; Soliman, Gihan M.; Tawfik, Sadika M.; Zaher, Shaimaa M.

doi:10.1016/j.jmau.2014.04.002

Cited by 2 publications

(1 citation statement)

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“…The increase in cytosolic calcium has a harmful effect on the cells through activation of caspases with subsequent cellular apoptosis. As well as through activation of protease enzymes with subsequent break down of membrane and cytoskeletal proteins which resulted in detachment and focal loss of microvilli [36,37] .…”

Section: Discussionmentioning

confidence: 99%

Lycopene reduces potassium bromate-induced structural alterations in the jejunal mucosa of adult rats

Bahey

Elswaidy

2020

Egyptian Journal of Histology

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Introduction: Potassium bromate (KBrO 3 ) is an oxidizing agent that is widely used as a flour improver. However, it induces genotoxic and carcinogenic effects on different body organs in a dose and duration-dependent manner. Aim of this Study:To explore the effects of KBrO 3 on the structure of the rat jejunal mucosa and investigate the potential role of lycopene, a strong antioxidant molecule, in preventing or ameliorating the effect of KBrO 3 . Materials and Methods: Twenty-four adult male albino rats were used and divided equally into four experimental groups; control group, lycopene group that received 10mg of lycopene/kg/day/orally; KBrO 3 group that received 100mg of KBrO 3 /kg/ day/ orally and KBrO 3 and lycopene group that received KBrO 3 and lycopene in the same doses as in the previous groups. Animals were sacrificed after 4 weeks and the specimens from the jejunum were processed for histological, immunohistochemical, and ultrastructural examinations. Results:The jejunal mucosa of the KBrO 3 treated group showed short and broad villi, discontinuity and desquamation of their lining epithelial cells, inflammatory cellular infiltration, and dilatation of the blood vessels. Moreover, there was a significant decrease in the number of goblet cells and PCNA immuno-stained nuclei in the jejunum. Ultramicroscopic examination showed swollen vacuolated mitochondria, dilated rough endoplasmic reticulum, and shrunken dark nuclei. Interestingly, the group treated with both lycopene and KBrO 3 showed less cytoplasmic vacuolation and mitochondrial abnormalities in the epithelial cells lining the villi. Furthermore, there was a significant improvement in the height of jejunal villi, the number of goblet cells, and PCNA immuno-stained nuclei. Conclusion: KBrO 3 induced cellular damage in the rat jejunal mucosa which was prevented by coadministration of lycopene.

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Section: Discussionmentioning

confidence: 99%