Evaluation of the Osteogenic Potential of Growth Factor–Rich Demineralized Bone Matrix In Vivo

Yu, Shan Huey; Chan, Hsun‐Liang; Li, Chong; Jheng, Yi Han; Chang, Ping-Chuan

doi:10.1902/jop.2014.140333

Cited by 10 publications

(9 citation statements)

References 30 publications

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“…However, in bone decellularization protocols, partial decalcification is often applied, mainly for handling purposes, but also for improving osteoinductive properties. Urist et al [ 32 ] showed decades ago that the decalcification of native bone is able to not only retain bone morphogenic proteins and growth factors entrapped in the bone matrix, but even to further expose them and therefore facilitate specific cell–ECM interactions [ 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

Modeling of the Human Bone Environment: Mechanical Stimuli Guide Mesenchymal Stem Cell–Extracellular Matrix Interactions

et al. 2021

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In bone tissue engineering, the design of in vitro models able to recreate both the chemical composition, the structural architecture, and the overall mechanical environment of the native tissue is still often neglected. In this study, we apply a bioreactor system where human bone-marrow hMSCs are seeded in human femoral head-derived decellularized bone scaffolds and subjected to dynamic culture, i.e., shear stress induced by continuous cell culture medium perfusion at 1.7 mL/min flow rate and compressive stress by 10% uniaxial load at 1 Hz for 1 h per day. In silico modeling revealed that continuous medium flow generates a mean shear stress of 8.5 mPa sensed by hMSCs seeded on 3D bone scaffolds. Experimentally, both dynamic conditions improved cell repopulation within the scaffold and boosted ECM production compared with static controls. Early response of hMSCs to mechanical stimuli comprises evident cell shape changes and stronger integrin-mediated adhesion to the matrix. Stress-induced Col6 and SPP1 gene expression suggests an early hMSC commitment towards osteogenic lineage independent of Runx2 signaling. This study provides a foundation for exploring the early effects of external mechanical stimuli on hMSC behavior in a biologically meaningful in vitro environment, opening new opportunities to study bone development, remodeling, and pathologies.

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Section: Discussionmentioning

confidence: 99%

Modeling of the Human Bone Environment: Mechanical Stimuli Guide Mesenchymal Stem Cell–Extracellular Matrix Interactions

et al. 2021

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“…DBM has excellent biological properties, osteoinductive, osteoconductive, biodegradability, promotes bone mineralization, and accelerates bone healing [46]. As an allograft bone graft, the natural advantages (Table 1) of decalcified bone are as follows: (1) Decalcification removes the dense mineral components which wrap around BMPs and other growth factors, consequently these factors can be released smoothly and exerts osteogenic activity [47,48]; (2) DBM is one of the weakest immunogens in alternative bone graft materials after being treated with various chemical agents; (3) DBM has the natural bone composition, reticular pore structure and 3D structure. It also has good affinity and binding force, so it can be used as a natural carrier of a variety of growth factors, which was conducive to seed cell adhesion and growth.…”

Section: Natural Bone Graftmentioning

confidence: 99%

Reconstructing Bone with Natural Bone Graft: A Review of In Vivo Studies in Bone Defect Animal Model

Liu

2018

Nanomaterials

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Bone defects caused by fracture, disease or congenital defect remains a medically important problem to be solved. Bone tissue engineering (BTE) is a promising approach by providing scaffolds to guide and support the treatment of bone defects. However, the autologous bone graft has many defects such as limited sources and long surgical procedures. Therefore, xenograft bone graft is considered as one of the best substitutions and has been effectively used in clinical practice. Due to better preserved natural bone structure, suitable mechanical properties, low immunogenicity, good osteoinductivity and osteoconductivity in natural bone graft, decellularized and demineralized bone matrix (DBM) scaffolds were selected and discussed in the present review. In vivo animal models provide a complex physiological environment for understanding and evaluating material properties and provide important reference data for clinical trials. The purpose of this review is to outline the in vivo bone regeneration and remodeling capabilities of decellularized and DBM scaffolds in bone defect models to better evaluate the potential of these two types of scaffolds in BTE. Taking into account the limitations of the state-of-the-art technology, the results of the animal bone defect model also provide important information for future design of natural bone composite scaffolds.

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“…Actually, the organic components of DBMs mainly consist of two parts: collagen protein and noncollagenous proteins. These noncollagenous proteins undertake almost all biological functions of DBMs, including osteoinductivity, which offers a considerable advantage to DBMs with respect to bone reconstruction . Bone morphogenetic protein 2 (BMP‐2), as one of these noncollagenous proteins in DBMs, is believed to promote the osteogenic differentiation of stem cells and formation of new bone .…”

Section: Introductionmentioning

confidence: 99%

“…These noncollagenous proteins undertake almost all biological functions of DBMs, including osteoinductivity, which offers a considerable advantage to DBMs with respect to bone reconstruction. 10,11 Bone morphogenetic protein 2 (BMP-2), as one of these noncollagenous proteins in DBMs, is believed to promote the osteogenic differentiation of stem cells and formation of new bone. [12][13][14][15] Moreover, BMP-2 is the only common link between the various forms of heterotopic ossification (HO).…”

Section: Introductionmentioning

confidence: 99%

Effects of hydrogen peroxide on biological characteristics and osteoinductivity of decellularized and demineralized bone matrices

Qing

Zhang

Yang

et al. 2019

J Biomedical Materials Res

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Due to the similar collagen composition and closely physiological relationship with soft connective tissues, demineralized bone matrices (DBMs) were used to repair the injured tendon or ligament. However, the osteoinductivity of DBMs would be a huge barrier of these applications. Hydrogen peroxide (H2O2) has been proved to reduce the osteoinductivity of DBMs. Nevertheless, the biological properties of H2O2‐treated DBMs have not been evaluated completely, while the potential mechanism of H2O2 compromising osteoinductivity is also unclear. Hence, the purpose of this study was to characterize the biological properties of H2O2‐treated DBMs and search for the proof that H2O2 could compromise osteoinductivity of DBMs. Decellularized and demineralized bone matrices (DCDBMs) were washed by 3% H2O2 for 12 h to fabricate the H2O2‐treated DCDBMs (HPTBMs). Similar biological properties including collagen, biomechanics, and biocompatibility were observed between DCDBMs and HPTBMs. The immunohistochemistry staining of bone morphogenetic protein 2 (BMP‐2) was negative in HPTBMs. Furthermore, HPTBMs exhibited significantly reduced osteoinductivity both in vitro and in vivo. Taken together, these findings suggest that the BMP‐2 in DCDBMs could be the target of H2O2. HPTBMs could be expected to be used as a promising scaffold for tissue engineering. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.

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Evaluation of the Osteogenic Potential of Growth Factor–Rich Demineralized Bone Matrix In Vivo

Abstract: GDBM demonstrated acceptable biocompatibility and osteogenic potential comparable to ABBM in vivo. Further investigations in a more clinically relevant model are warranted.

Cited by 10 publications

References 30 publications

Modeling of the Human Bone Environment: Mechanical Stimuli Guide Mesenchymal Stem Cell–Extracellular Matrix Interactions

Modeling of the Human Bone Environment: Mechanical Stimuli Guide Mesenchymal Stem Cell–Extracellular Matrix Interactions

Reconstructing Bone with Natural Bone Graft: A Review of In Vivo Studies in Bone Defect Animal Model

Effects of hydrogen peroxide on biological characteristics and osteoinductivity of decellularized and demineralized bone matrices

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