Evaluation of the natural killer cytotoxicity and the levels of cytokines in rats with type I diabetes mellitus

Fidan, Işıl; Yüksel, Sevgi; Kalkancı, Ayşe; İmir, Turgut; Kuştımur, Semra

doi:10.1590/s0074-02762005000800010

Cited by 9 publications

(6 citation statements)

References 20 publications

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“…In their study on female mice with diabetes after 6–8 weeks of STZ, Fidan et al (2005) found an increase in the level of serum IFN-γ with regard to the control group and that the levels of serum TNF-α, IL-1, IL-2, IL-6 and IL-10 remained stable.…”

Section: Discussionmentioning

confidence: 97%

Effect of Lycopene Application in Rats with Experimental Diabetes Using Lipoprotein, Paraoxonase and Cytokines

2013

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This study was conducted with the purpose of researching the effect of lycopene application on lipoprotein, paraoxonase (PON) and cytokines that are projected to be used in the diagnosis and treatment of diabetes by making experimental diabetes. At the end of a 1-month trial period, under ether anesthesia with jelly tubes, blood samples were taken from rat hearts. Blood samples were centrifuged and serum was obtained. From the serum samples, HbA1c, paraoxonase activity, lipoprotein levels and cytokines were determined. HbA1c levels and PON activity were found to be p < 0.001. At the triglyceride level, with regard to the control group, in all the groups a significant rise occurred (p ≤ 0.001). At the cholesterol level, with regard to the control group, a decline was observed in the other groups (p < 0.05). At the VLDL level, with regard to the control group, a significant rise was observed in the other groups (p < 0.05). At the HDL (p < 0.001) and LDL (p < 0.05) levels, with regard to the control group, a significant decline was observed in the other groups. At the TNF-α, IL-2, IL-6 and IL-10 levels no difference was found (p > 0.05). Experimental diabetes models have an important place in analyzing diabetes complications and determining treatment approaches.

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Section: Discussionmentioning

confidence: 97%

Effect of Lycopene Application in Rats with Experimental Diabetes Using Lipoprotein, Paraoxonase and Cytokines

2013

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“…Immunological abnormalities such as abnormal expression of NK cell surface markers and abnormal cytokine production may also be associated with the pathogenesis of DM. In fact, some researchers had reported decreased expression of NKp46 and changed levels of serum cytokines such as IFN‐γ, TNF‐α, TGF‐β, and VEGF in patients with DM . Recently, the relationship between immune response and metabolic diseases such as obesity, type 2 diabetes, and GDM was reported …”

Section: Discussionmentioning

confidence: 99%

“…For the onset of type 1 DM, NK cell activity was diverse . On the other hand, NK cell cytotoxicity decreased in long‐standing type 1 DM group than that in the non‐DM healthy group . Patients with type 2 diabetes mellitus (DM) and impaired glucose tolerance (IGT) had also significantly lower levels of NK cell activity compared to that of the normal controls in both men and women .…”

Section: Discussionmentioning

confidence: 99%

Expression of Natural Cytotoxicity Receptors on and Intracellular Cytokine Production by NK Cells in Women with Gestational Diabetes Mellitus

Chiba

Fukui

Fuchinoue

et al. 2016

American J Rep Immunol

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Gestational diabetes mellitus (GDM) is characterized by glucose intolerance that is caused by metabolic changes during pregnancy, and is considered a risk factor for the occurrence of DM in future. There are studies that reported changes in the cytokine in women with GDM. The concentration of serum IFN-γ and adiponectin decreased, while serum leptin, IL-6, IL-10, and TNF-α increased in GDM women when compared to that in healthy pregnant women. 16 17 Placenta and adipose tissues from patients with GDM released higher amounts of TNF-α in response to high glucose than that from normal pregnant women.18 Plasma TNF-α were reported to be low during the first and second trimester, but it increased during late pregnancy, and was inversely correlated with the insulin sensitivity. 19, 20 However, the relationship between GDM and the functions of NK cells in pregnant women has not been clarified so far. The purpose of this study was to evaluate the expression of peripheral blood NK (pNK) cell surface markers (CD16, NKp46, and NKp30) and the percentage of cytokine (IFN-γ, TNF-α, TGF-β, and VEGF) producing pNK cells in women with / without GDM at 12 weeks of pregnancy. 2 Materials and Methods Study SubjectsWe designed a prospective study and patients from Hirosaki University Hospital were recruited for this study between July 2014 and March 2015. This study was approved by the local ethics committee, and signed informed consent forms were obtained from all study subjects. Blood samples were collected during routine blood tests for prenatal check-up at 12weeks of gestation.Thirty-four Japanese women (GDM (n=7), non-GDM (n=27)) with singleton pregnancy were included. All GDM patients were diagnosed at 12 to 16 weeks of gestation. Pregnant women with multi-fetal gestations, pre-gestational diabetes, and overt diabetes were excluded, while pregnant women with impaired glucose tolerance before the onset of pregnancy were included in this study. Maternal and neonatal characteristics are shown in Table 1. Two-hour-glucose level of 75-g OGTT in GDM group was significantly higher than that in the non-GDM group.There were no complications reported in the newborn infants (such as macrosomia, shoulder dystocia, and hypoglycemia) in both the GDM and non-GDM groups and there were no pregnant women suffering from PE in both the GDM and non-GDM groups. Screening of GDMAll pregnant women were subjected to a two-step screening method for GDM. This screening method is based on the Guideline for Obstetrical Practice in Japan 2014. 21 Briefly, women who had a random plasma glucose level of ≥95mg/dl at 12 weeks of gestation were subjected to 75-g OGTT after fasting overnight. Diagnosis of GDM was based on the criteria of one or more abnormal values [fasting ≥92mg/dl (5.1mmol/l), such as 1 hour ≥180mg/dl (10.0mmol/l) or 2 hour ≥153mg / dl (8.5mmol/l)] reported for the subjects. Pregnant women, not diagnosed as GDM at 12 weeks of screening, were again subjected to random blood glucose measurement at 3 28 weeks of gestation. Women with random...

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“…CFA causes a rapid increase in the percentage of NK cells and their secretion of IFN-γ [ 57 ]. Similar results were obtained by Fidan et al ., who noted a higher concentration of IFN-γ among rats with T1DM [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Lower percentages of natural killer cells in children with type 1 diabetes and their siblings

Sieniawska,

Krzewska,

Skowronek

et al. 2023

pedm

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Introduction One of the most common children’s endocrine and autoimmune diseases in the world is type 1 diabetes mellitus (T1DM). The incidence of type 1 diabetes is constantly increasing, and according to current estimates, the number of children with T1DM in the world has exceeded 542,000. There are 3 main components emphasized in the pathogenesis: genetic and environmental factors, and the patient's immune system. Many publications have confirmed the role of natural killer cells (NK) in the pathogenesis of type 1 diabetes and other autoimmune diseases. Aim The aim of the study was to evaluate the population of NK cells and pancreatic β cell autoantibodies in a group of children with T1DM and their healthy siblings in comparison with children from families with no history of autoimmune diseases. Material and methods The research included 76 children with T1DM, 101 children from the sibling group, and 30 children from the control group. Peripheral blood was analysed on a FACSCalibur flow cytometer (Becton Dickinson) to evaluate the NK cell population. The results were presented as the percentage of NK cells among lymphocytes. Statistical analysis was performed using STATISTICA 10 PL software. Results The mean percentage of NK cells in children with T1D (10.59 ±5.37) and in the sibling group (11.93 ±5.62) was statistically reduced in comparison to the control group (14.89 ±7.78) in sequence (Student’s t -test: t = –3.24; df = 103; p = 0.002) (Student’s t -test: t = –2.30; df = 128; p = 0.02). There was no statistically significant difference in the percentage of NK cells between the group of children with T1DM and their siblings (Student’s t -test: t = –1.59; df = 173; p = 0.11). In the group of siblings, the younger the child, the lower the reported percentage of NK cells. This relationship was statistically significant (test for the Pearson correlation coefficient t = 3.41; p = 0.0009; r = 0.33). In the group of children with type 1 diabetes, a similar relationship was not found. The concentration of anti-IA2 and anti-Znt8 antibodies was statistically significantly higher in the sibling group compared to the control group (anti-IA2 p = 0.0000001; anti-ZnT8 p = 0.00001), and the concentration of anti-GAD antibodies was comparable in both groups. In the group of children with type 1 diabetes, a positive correlation was demonstrated between the reduced percentage of NK cells and the coexistence of anti-GAD and anti-ZnT8 antibodies (Mann-Whitney U ...

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Evaluation of the natural killer cytotoxicity and the levels of cytokines in rats with type I diabetes mellitus

Cited by 9 publications

References 20 publications

Effect of Lycopene Application in Rats with Experimental Diabetes Using Lipoprotein, Paraoxonase and Cytokines

Effect of Lycopene Application in Rats with Experimental Diabetes Using Lipoprotein, Paraoxonase and Cytokines

Expression of Natural Cytotoxicity Receptors on and Intracellular Cytokine Production by NK Cells in Women with Gestational Diabetes Mellitus

Lower percentages of natural killer cells in children with type 1 diabetes and their siblings

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