Evaluation of the MBT STAR-Carba Assay for the Detection of Carbapenemase Production in <i>Enterobacteriaceae</i> and <i>Hafniaceae</i> with a Large Collection of Routine Isolates from Plate Cultures and Patient-Derived Positive Blood Cultures

Cordovana, Miriam; Abdalla, M. Sebawe; Ambretti, Simone

doi:10.1089/mdr.2019.0466

Cited by 7 publications

(6 citation statements)

References 68 publications

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“…This method is a phenotypic assay, comparing microbial growth after incubation in the absence or presence of different concentrations of antimicrobial agents. Cell growth is estimated from a comparison of areas under the curve of the MALDI-TOF MS spectra of different incubation conditions [ 78 , 79 , 80 ].…”

Section: Maldi-tof Ms Combined With Other Methodsmentioning

confidence: 99%

Current Status of Matrix-Assisted Laser Desorption/Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in Clinical Diagnostic Microbiology

2020

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Mass spectrometry (MS), a core technology for proteomics and metabolomics, is currently being developed for clinical applications. The identification of microorganisms in clinical samples using matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) is a representative MS-based proteomics application that is relevant to daily clinical practice. This technology has the advantages of convenience, speed, and accuracy when compared with conventional biochemical methods. MALDI-TOF MS can shorten the time used for microbial identification by about 1 day in routine workflows. Sample preparation from microbial colonies has been improved, increasing the accuracy and speed of identification. MALDI-TOF MS is also used for testing blood, cerebrospinal fluid, and urine, because it can directly identify the microorganisms in these liquid samples without prior culture or subculture. Thus, MALDI-TOF MS has the potential to improve patient prognosis and decrease the length of hospitalization and is therefore currently considered an essential tool in clinical microbiology. Furthermore, MALDI-TOF MS is currently being combined with other technologies, such as flow cytometry, to expand the scope of clinical applications.

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Section: Maldi-tof Ms Combined With Other Methodsmentioning

confidence: 99%

Current Status of Matrix-Assisted Laser Desorption/Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in Clinical Diagnostic Microbiology

2020

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“…Although carbapenem resistance can occur in several ways, enzymatic hydrolysis of an antibiotic (carbapenemase expression) is considered of more clinical concern since CPB demonstrate the greatest potential for rapid dissemination within hospital settings via horizontal transfer of the carbapenemase-encoding genes as well as clonal expansion [8,9]. Regarding Ambler classification, carbapenemases are classified into 3 main groups-class A serine β-lactamases mostly represented by KPC (Klebsiella pneumoniae carbapenemase), class B metallo-βlactamases-MBL (e.g.…”

Section: Introductionmentioning

confidence: 99%

Study on carbapenemase-producing bacteria by matrix-assisted laser desorption/ionization approach

et al. 2021

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The development of new techniques for the detection of carbapenemase activity is of great importance since the increased incident of resistance against carbapenems represents a serious threat to global public health. In this context, the matrix-assisted laser desorption/ionization approach already demonstrated to be a reliable tool for rapid carbapenemase detection. As a newly developed test, there is still a lack of in-depth analysis of its robustness and possible wider application. The main goal of this study was to evaluate the potential for using the design MBT STAR-Carba assay as the pre-characterization method for Enterobacterales and P. aeruginosa strains in terms of the produced classes of carbapenemases using modified procedure parameters—various suspension densities and incubation times. Moreover, its usefulness for the in-depth analysis and characterization of metallo-β-lactamases (MBL) was tested by applying inhibition assays. In this study, the designed assay proved to be a sensitive tool for the detection of carbapenemase hydrolytic activity, which can be successfully used to partially classify the class of carbapenemase present. Additionally, the use of defined high concentration suspensions would allow to shorten the incubation time to 1 minute for certain strains. Considering that the assay was also suitable to investigate the effect of different inhibitors on the MBL activity, it demonstrates far higher discriminatory potential than only a rapid routine carbapenemase detection tool and could be used as a susceptibility assay.

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“…The family consists of a single genus, Hafnia, which contains only one species, Hafnia alvei. This European native tiny annual herb may be found in a range of environments, including grasslands, meadows and cultivated fields (Cordovana et al 2020). Hafnia alvei is not typically used for any medicinal or culinary purposes, but it is known to produce a yellow pigment called hafnium.…”

Section: Hafniaceaementioning

confidence: 99%

Incidence, Transmission Mechanisms and Pathologic Implications of Bacterial Zoonotic Diseases of Fish

2023

IJAB

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Fish harbor a variety of bacterial pathogens, some of which can cause disease in humans. Zoonotic bacteria that may be present in asymptomatic fish hosts pose a public health risk when transmitted to humans through handling or consuming infected fish. Mycobacteriosis, caused by species in the Mycobacterium marinum group, is perhaps the most notorious zoonotic infection associated with fish and can produce serious skin infections in humans. Streptococcosis and staphylococcosis also occur with some frequency in fish and can result in human cases of septicemia, endocarditis or pneumonia if injured skin comes into contact with infected fish tissues and bacteria access wounds. Additionally, some Vibrio and Clostridium species found among fish may cause wound infections or gastrointestinal illness in humans, usually subsequent to exposure through handling or ingesting raw seafood. Clinical signs of bacterial zoonosis are variable and diagnosis in human cases can prove complicated by the vast diversity of potential pathogens involved. Preventative measures center on educating aquarists and fish handlers to avoid direct contact with ulcerated areas, lesions or feces from diseased fish. For consumer safety, good aquaculture practices that reduce bacterial loads in farmed fish stock are recommended. Moreover, thoroughly cooking fish to an internal temperature over 140°F destroys pathogens that may be present. Additional research priorities include better characterization of bacterial diversity among wild and farmed fish, investigating genetic and immunological aspects of disease resistance, developing improved diagnostics through genomic analysis, assessing efficacy of existing antibacterial treatments in clearing pathogens prior to human consumption and formulating integrated control strategies to mitigate risks. This book chapter examines the incidence, transmission mechanisms and pathologic implications of common bacterial zoonosis originating from fish hosts.

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Evaluation of the MBT STAR-Carba Assay for the Detection of Carbapenemase Production in Enterobacteriaceae and Hafniaceae with a Large Collection of Routine Isolates from Plate Cultures and Patient-Derived Positive Blood Cultures

Cited by 7 publications

References 68 publications

Current Status of Matrix-Assisted Laser Desorption/Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in Clinical Diagnostic Microbiology

Current Status of Matrix-Assisted Laser Desorption/Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in Clinical Diagnostic Microbiology

Study on carbapenemase-producing bacteria by matrix-assisted laser desorption/ionization approach

Incidence, Transmission Mechanisms and Pathologic Implications of Bacterial Zoonotic Diseases of Fish

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