Current Status of Matrix-Assisted Laser Desorption/Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in Clinical Diagnostic Microbiology

Tsuchida, Sachio; Umemura, Hiroshi; Nakayama, Tomohiro

doi:10.3390/molecules25204775

Cited by 120 publications

(114 citation statements)

References 89 publications

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“…However, the correct identification of filamentous fungi remains challenging even when using the ethanol-formic acid extraction method. 2,3 Several studies have reported the direct identification of pathogens from blood and urine samples without prior culturing that could be used for rapid diagnosis. [4][5][6] These innovations have resulted in a major decrease in the times required for species identification to several minutes compared to around one day using traditional biochemical identification.…”

Section: Introductionmentioning

confidence: 99%

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A Rapid Antimicrobial Susceptibility Test for Klebsiella pneumoniae Using a Broth Micro-Dilution Combined with MALDI TOF MS

Wang

Song

2021

IDR

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Background: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a novel method that can be used to identify pathogens and has potential applications in the detection of drug-resistant bacteria. Purpose: To evaluate the ability of a MALDI-TOF MS-based broth micro-dilution method in detecting the minimum inhibitory concentration (MIC) values of Klebsiella pneumoniae to ceftriaxone and imipenem. Materials and Methods: Sixty strains of K. pneumoniae with different levels of resistance to carbapenems and cephalosporins were randomly collected. The 0.5 McFarland (Mc) concentration of the bacterial suspension was inoculated in cation-adjusted Mueller-Hinton broth (CAMHB) with a final cell turbidity of 5×10 5 CFU/mL. The broth was incubated with serial concentrations of antibiotics. After centrifuging the bacterial suspensions, the lysed cells were analyzed by MALDI-TOF MS to identify the growth-promoting or inhibitory effects on K. pneumoniae. The molecular mechanisms of resistance were investigated by PCR and DNA sequencing analysis. Results: The expression of known resistance genes (blaKPC, blaFOX, blaDHA, blaCTX-M and blaTEM) was detected in the 30 carbapenems-resistant strains. The agreement between the MIC values derived from the MALDI-TOF MS analysis and from the broth microdilution method was 61.7% for ceftriaxone and 71.7% for imipenem. According to the Clinical and Laboratory Standards Institute (CLSI) breakpoint of resistance to ceftriaxone and imipenem, the 60 isolates were accurately classified as resistant or susceptible isolates with 100% sensitivity and 100% specificity. Conclusion:The transmission and infection of multidrug-resistant bacteria could be better managed and treated with the rapid identification of strains and antimicrobial susceptibility. A MALDI-TOF MS-based susceptibility test could be used to identify resistance of K. pneumoniae within a short time-frame. This approach could potentially be used as a supplementary antimicrobial susceptibility test that could be investigated on more bacterial species combined with different antibiotics.

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Section: Introductionmentioning

confidence: 99%

“…However, the correct identification of filamentous fungi remains challenging even when using the ethanol-formic acid extraction method. 2 , 3 …”

Section: Introductionmentioning

confidence: 99%

A Rapid Antimicrobial Susceptibility Test for Klebsiella pneumoniae Using a Broth Micro-Dilution Combined with MALDI TOF MS

Wang

Song

2021

IDR

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“…In our study, we tried to improve the identification of 151 strains not identifiable by the MALDI-TOF with the direct transfer procedure. We showed that acid extraction would not improve the identification of these strains, which was surprising in view of the literature (Tsuchida et al, 2020). Among the 124 strains identified at Bruker scores below 1.7, only 41.9% were correctly identified at the genus level, and among these strains, only three strains were correctly identified at the species level by both techniques.…”

Section: Discussionmentioning

confidence: 63%

“…The primary recommended method for obtaining bacterial IDs from colonies is the cell smear (direct transfer) method. This smear method has shown its efficiency for the identification of numerous bacteria (Schröttner et al, 2014;Tsuchida et al, 2020); however, the probability of correctly identifying certain bacteria can still be quite limited (Tsuchida et al, 2020). If the mass spectrum obtained by the plate method is not sufficient, the acid extraction method can be applied to improve identification.…”

Section: Discussionmentioning

confidence: 99%

MALDI-TOF Mass Spectrometry Fingerprinting Performance Versus 16S rDNA Sequencing to Identify Bacterial Microflora From Seafood Products and Sea Water Samples

et al. 2021

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We evaluated the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) associated with the Bruker BioTyperTM V7.0.0 database for the identification of 713 bacterial strains isolated from seafood products and sea water samples (ANSES B3PA collection) under culture conditions that may have been significantly different from those used to create the reference spectrum vs. the 16S rDNA sequencing. We identified 78.8% of seafood isolates with 46.7% at the species level (Bruker score above 2) and 21.2% (Bruker score between 1.7 and 2) at the genus level by the two identification methods, except for 3.8% of isolates with a difference of identification between the two methods (Bruker score between 1.7 and 2). There were 41.9% isolates (Bruker score below 1.7) with the identification at the genus level. We identified 94.4% of seafood isolates with 16S rDNA sequencing. The MALDI-TOF allowed a better strain identification to the species level contrary to the 16s rDNA sequencing, which allowed an identification mainly to the genus level. MALDI-TOF MS in association with the Bruker database and 16S rDNA sequencing are powerful tools to identify a wide variety of bacteria from seafood but require further identification by biochemical, molecular technique or other conventional tests.

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“…Bacterial meningitis is a neurological emergency. For both sepsis and meningitis, early diagnosis is vital for rapid initiation of antimicrobial treatment [ 24 ]. Direct detection and identification of microorganisms from blood and cerebrospinal fluid (CSF) are a relevant step.…”

Section: Direct Microbial Identification From Human Samplesmentioning

confidence: 99%

Application and Perspectives of MALDI–TOF Mass Spectrometry in Clinical Microbiology Laboratories

2021

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Early diagnosis of severe infections requires of a rapid and reliable diagnosis to initiate appropriate treatment, while avoiding unnecessary antimicrobial use and reducing associated morbidities and healthcare costs. It is a fact that conventional methods usually require more than 24–48 h to culture and profile bacterial species. Mass spectrometry (MS) is an analytical technique that has emerged as a powerful tool in clinical microbiology for identifying peptides and proteins, which makes it a promising tool for microbial identification. Matrix assisted laser desorption ionization–time of flight MS (MALDI–TOF MS) offers a cost- and time-effective alternative to conventional methods, such as bacterial culture and even 16S rRNA gene sequencing, for identifying viruses, bacteria and fungi and detecting virulence factors and mechanisms of resistance. This review provides an overview of the potential applications and perspectives of MS in clinical microbiology laboratories and proposes its use as a first-line method for microbial identification and diagnosis.

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Current Status of Matrix-Assisted Laser Desorption/Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in Clinical Diagnostic Microbiology

Cited by 120 publications

References 89 publications

A Rapid Antimicrobial Susceptibility Test for Klebsiella pneumoniae Using a Broth Micro-Dilution Combined with MALDI TOF MS

A Rapid Antimicrobial Susceptibility Test for Klebsiella pneumoniae Using a Broth Micro-Dilution Combined with MALDI TOF MS

MALDI-TOF Mass Spectrometry Fingerprinting Performance Versus 16S rDNA Sequencing to Identify Bacterial Microflora From Seafood Products and Sea Water Samples

Application and Perspectives of MALDI–TOF Mass Spectrometry in Clinical Microbiology Laboratories

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