Evaluation of the J-Plasma Electrosurgical Device Combined with Nebulized Collagen for Burn Healing in Rodents

Abstract

numerous areas. Much of the work has been carried out using innovative devices, but there remains markets such as the United States. Although a wide variety of argon plasma coagulators are approved for medical use, these devices have not been considered to be viable for plasma medicine applications due to the high thermal energy of argon discharges. We explore the potential of a commercial helium plasma device to deliver low-energy, nonthermal plasma discharges to wound sites. The recently launched J-Plasma device (Apyx Medical; Clearwater, FL, USA) uses low-temperature helium plasma and delivers precise plasma power with minimal depth of thermal spread. We investigate the impact of J-Plasma on nebulized biologic materials and its minimal impact on protein chemical structure. We discuss the potential to deliver biologic materials and control in vivo data from a rodent burn model.

Plasma deposition of collagen for cell‐culture applications

“…Spectra were collected from both sets of NaCl plates, and the results are shown in Figure . The spectra are both highly similar and there is no evidence of any significant difference between the plasma‐deposited collagen and the air‐dried sample, as per previous studies involving similar systems . At higher wavenumbers, the spectra are dominated by the amide N–H stretching vibrations at 3,325 and 3,086 cm −1 .…”

“…Discharges produced using high energies or oxidising gases are particularly effective at denaturing biomolecules and the damage is typically dose‐dependent. Despite this, previous studies have shown that short term contact of a sprayed liquid solution with nonthermal helium plasma discharges does not dramatically alter the chemistry or biological activity of various proteins . Heyse et al have postulated that the liquid droplet may protect the dissolved protein from direct contact with the more aggressive plasma species.…”

“…Recent research has shown that plasma processing can effectively deposit protein layers in a single step and that the coatings can retain biological activity . Of specific note are the reports that type I collagen layers have been applied as thin film coatings without additional polymers, binders, or linkers, and these have demonstrated effective healing properties in vivo . Given the prevalence of plasma processing in the diagnostic and tissue culture market, this one‐step collagen‐coating process has the potential to combine the TCT, sterilisation and protein coating steps into a single‐production process.…”

Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method

“…Numerous publications have now determined that such atmospheric helium plasma systems are capable of depositing functionally intact coatings on a range of surfaces [21]. Recent studies have shown that such plasma systems are also capable of depositing functional biological coatings [22] and in particular collagen coatings [23,36]. This may be in part limited to the transient nature of the contact with the helium plasma, which minimizes the potential for process mediated structural modifications.…”

“…Significantly, this approach may also be applicable to wound care. By using a cold atmospheric pressure plasma device to deposit biologics, it may be possible to combine the benefits of plasma medicine [39][40][41][42][43] with targeted local delivery of biologic therapeutics at a wound site [36]. Such an approach could allow for significant benefits across a range of clinical conditions.…”