Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method

O'Sullivan, Denis; McArdle, Hazel; Ng, Sing Wei; Bourke, Paula; Forster, Robert J.; O’Neill, Liam

doi:10.3390/app10196670

Cited by 4 publications

(2 citation statements)

References 42 publications

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“…Instead of polystyrene, the plates referred to as “Untreated”, “TA”, “TB”, “TC” and “TD” were manufactured with Crystal Zenith® (CZ) COP. These plates were treated with cold-deposition plasma (TA), coated with collagen type I (TB), fibronectin (TC), or anti-CD3 (TD), using a cold plasma deposition method described in detail elsewhere (O’Sullivan et al 2020a , b , c ). In summary, plasma deposition was carried out using a Biodep unit manufactured by TheraDep Inc.…”

Section: Methodsmentioning

confidence: 99%

Understanding the impact of bioactive coating materials for human mesenchymal stromal cells and implications for manufacturing

et al. 2023

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Bioactive materials interact with cells and modulate their characteristics which enable the generation of cell-based products with desired specifications. However, their evaluation and impact are often overlooked when establishing a cell therapy manufacturing process. In this study, we investigated the role of different surfaces for tissue culture including, untreated polystyrene surface, uncoated Cyclic Olefin Polymer (COP) and COP coated with collagen and recombinant fibronectin. It was observed that human mesenchymal stromal cells (hMSCs) expanded on COP-coated plates with different bioactive materials resulted in improved cell growth kinetics compared to traditional polystyrene plates and non-coated COP plates. The doubling time obtained was 2.78 and 3.02 days for hMSC seeded in COP plates coated with collagen type I and recombinant fibronectin respectively, and 4.64 days for cells plated in standard polystyrene treated plates. Metabolite analysis reinforced the findings of the growth kinetic studies, specifically that cells cultured on COP plates coated with collagen I and fibronectin exhibited improved growth as evidenced by a higher lactate production rate (9.38 × 105 and 9.67 × 105 pmol/cell/day, respectively) compared to cells from the polystyrene group (5.86 × 105 pmol/cell/day). This study demonstrated that COP is an effective alternative to polystyrene-treated plates when coated with bioactive materials such as collagen and fibronectin, however COP-treated plates without additional coatings were found not to be sufficient to support cell growth. These findings demonstrate the key role biomaterials play in the cell manufacturing process and the importance of optimising this selection.

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Section: Methodsmentioning

confidence: 99%

Understanding the impact of bioactive coating materials for human mesenchymal stromal cells and implications for manufacturing

et al. 2023

Self Cite

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“…Significantly, the cold plasma method preserved both the primary and secondary structure of the protein and this resulted in high biocompatibility and cell activity that was at least equivalent to the standard wet chemical technique. The significance of these results is discussed in relation to the benefits of a single step plasma coating in comparison to the traditional multi-step aseptic coating technique [4].…”

Section: Surface Functionalization and Coating Of Biomedical Materialsmentioning

confidence: 98%

Application of Plasma Technology in Bioscience and Biomedicine

Boehm

Canal

2021

Applied Sciences

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Plasma surface functionalization: A comprehensive review of advances in the quest for bioinstructive materials and interfaces

Zhianmanesh

Gilmour

Bilek

et al. 2023

Applied Physics Reviews

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Surface biofunctionalization aims to create cell-instructive surfaces that control the behavior of cells and modulate cellular interactions by incorporating cell signaling moieties at the materials–biosystem interface. Despite advances in developing bioinert and biocompatible materials, blood clotting, inflammation, and cell death continue to be observed upon the contact of foreign materials with living tissues leading to the materials' rejection. Specific examples include the application of foreign materials in implantable devices (e.g., bone implants, antimicrobial surfaces, and cardiovascular stents), biosensors, drug delivery, and 3D-bioprinting. Biofunctionalization of materials to date has been predominantly realized using wet chemical approaches. However, the complexity of wet chemistry, toxicity of reactants, waste disposal issues, reaction time, poor reproducibility, and scalability drive a need for a paradigm shift from wet chemical approaches to dry methods of surface biofunctionalization. Plasma-based technologies that enable covalent surface immobilization of biomolecules have emerged as dry, reagent-free, and single-step alternatives for surface biofunctionalization. This review commences by highlighting the need for bioinstructive surfaces and coatings for various biomedical applications such as bone implants, antimicrobial surfaces, biosensors, and 3D-bioprinted structures, followed by a brief review of wet chemical approaches for developing biofunctionalized surfaces and biomimetic devices. We then provide a comprehensive review of the development of plasma-based technologies for biofunctionalization, highlighting the plasma–surface interactions and underpinning mechanisms of biomolecule immobilization.

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Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method

Cited by 4 publications

References 42 publications

Understanding the impact of bioactive coating materials for human mesenchymal stromal cells and implications for manufacturing

Understanding the impact of bioactive coating materials for human mesenchymal stromal cells and implications for manufacturing

Application of Plasma Technology in Bioscience and Biomedicine

Plasma surface functionalization: A comprehensive review of advances in the quest for bioinstructive materials and interfaces

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