Evaluation of the influence of the antibiotic ciprofloxacin in the development of an Old World screwworm fly, Chrysomya putoria

Abstract

Chrysomya putoria (Wiedemann) (Diptera: Calliphoridae), an Old World screwworm fly, is a species with potential for maggot therapy practice and has been described in myiasis and forensic entomology studies. The objective of the present study was to assess the action of different ciprofloxacin concentrations on the growth and development of C. putoria . First instar maggots of the third generation were raised on 60 g of chicken gizzard homogenate in 65% agar diet and received ciprofloxacin chloridrate. Each concentration of the antibiotic tested (3.33 µg/mL, 6.66 µg/mL, and 13.33 µg/mL) and the control (no antibiotic) were replicated four times (40 maggots/replication). The control received distilled water instead of the antibiotic. Maggots were kept in an acclimatized chamber at 30° C during the day and 28° C at night, with 70 + 10% RH and a 14:10 L:D photoperiod. They were weighed in batches of five and stored in test tubes sealed with nylon fabric and elastic. Microsoft Excel and STAT were used for the analysis. The variation among the maggot weight means and the duration of the maggot stage, pupal stage, and time to total development (neolarvae to adult) were analyzed by Student’s t -test (α= 5%). The viabilities and the normality rates were compared using ANOVA, and the expected sex ratio frequency was tested by the chisquared test (χ 2 ). There was no significant difference among the four treatments regarding mean individual maggot weight, mean duration of the maggot inoculation until abandonment, the duration of the maggot and pupal stages, and the total duration of all stages. The sex ratios found in the four treatments did not differ from the expected. Only treatment 2 (6.66 µg/mL concentration of ciprofloxacin) differed significantly from the control in maggot and total viability. The antibiotic did not seem to alter C. putoria development in the postembryonic period.

Post‐embryonic development of Chrysomya putoria (Diptera: Calliphoridae) in swine and bovine tissues in South America: implications for forensic entomology

“…On this subject, Ferraz et al . (, b; ) tested the development of C . putoria using a diet‐containing antibiotics (ampicillin, gentamicin and ciprofloxacin) in different concentrations, to study the species' growth rate under the effect of these substances.…”

Effect of Ciprofloxacin on the Composition of Intestinal Microbiota in Sarcophaga peregrina (Diptera: Sarcophagidae)

“…Fascinating studies on insect growth and development have uncovered that ciprofloxacin possesses the ability to neutralize Bacillus sphaericus, thereby fostering the proliferation of Aedes (Stegomyia) aegypti larvae [24]. Similarly, in the realm of Chrysomya pretoria, Ferraz et al [25] unearthed a stimulatory effect of ciprofloxacin on growth. Conversely, the metamorphosis of Anopheles barbirostris (Diptera: Culicidae) from larvae to adult is notably hindered by the administration of ciprofloxacin [26].…”

The multilevel antibiotic-induced perturbations to biological systems: Early-life exposure induces long-lasting damages to muscle structure and mitochondrial metabolism in flies

“…Surprisingly, most of the studies have focused on the fitness consequences of adding of these drugs to the food of laboratory cultured insects (Büyükgüzel & Kalender, 2008;Ridley et al, 2013;Xu et al, 2016). At low concentrations of ciprofloxacin (3.33 to 13.33 µg/mL), the development of the fly Chrysomia putoria was not altered (Ferraz et al, 2014), while addition of streptomycin to the food at concentrations of 0.03 to 0.15% increased the growth of the larvae of the butterfly Spodoptera litura, without affecting its survival (Thakur et al, 2016). Dickel et al (2016) reported that long-term exposure to prophylactic treatments (fumagillin) negatively affects the number of eggs laid by females of the wood tiger moth Parasemia plantaginis.…”