Evaluation of the influence of different doses of pre-sowing irradiation of seeds on the content of flavonoids in Hypericum perforatum L. medicinal raw material

Salivon, A. G.; Lystvan, K. V.; Litvinov, S. V.; Pchelovska, S.A.; Shylina, Yu. V.; Жук, В. В.; Tonkal, L. V.

doi:10.7124/feeo.v25.1183

Fakt. eksp. evol. org.

2019

DOI: 10.7124/feeo.v25.1183

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Evaluation of the influence of different doses of pre-sowing irradiation of seeds on the content of flavonoids in Hypericum perforatum L. medicinal raw material

A. G. Salivon¹,

K. V. Lystvan²,

S. V. Litvinov³

et al.

Abstract: Aim. The purpose of this work was to determine the dose of g- and X-ray pre-sowing irradiation of seeds of Hypericum perforatum L., causing an increase in biomass and the accumulation of flavonoids in medicinal raw materials. Methods. X-ray and g-irradiation of seeds, biometric methods, flavonoids extraction, quantification of flavonoid content in extracts using spectrophotometry, qualitative and semi-quantitative analysis of extracts using high-performance liquid chromatography (HPLC), common statistical meth… Show more

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Cited by 2 publications

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Productivity of common Saint-John's wort (Hypericum perforatum L.) by using transplant reproduction method in the conditions of drip irrigation

Pryvedenyuk¹,

Shatkovskyi

2021

LRaWM

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The influence of plant nutrition area and mineral fertilizer rates on the productivity of St. John's wort (Hypericum perforatum L.) by using transplant reproduction method in the conditions of drip irrigation was studied. It was proved that the transplant method of cultivation of St. John's wort under drip irrigation is a very effective method of reproduction of this crop. Four variants of planting density per unit area were studied: 42 thousand plants / ha (cultivation scheme 60x40 cm), 56 thousand plants / ha (60x30 cm), 83 thousand plants / ha (60x20 cm) and 167 thousand plants / ha (60x10 cm). Yield recording of raw materials (air-dry tops) was carried out in the phase of mass flowering. In the first year of vegetation this period was in the first decade of August, in the second year – in the second decade of June. It was found that the increase in the number of planted plants of St. John's wort per 1 ha contributed to the increased plantation productivity. When having a cultivation plant density of 42,000 plants / ha, the yield of dry grass in the first year of vegetation was 3,02 t / ha. Increasing the number of plants to 56 thousand plants / ha provided 3,26 t / ha of raw materials. The highest yield of dried St. John's wort – 3,76 t / ha in the first year of vegetation was obtained in the variant with the largest number of planted plants per unit area - 167 thousand plants / ha. In the second year of vegetation of St. John's wort in the variant with the lowest plant density of 42 thousand plants / ha, the yield was 3,65 t / ha. The most productive plantation of the second year of vegetation was in the variant with a plant density of 83 thousand plants / ha, where the yield of dry raw materials was 3,96 t / ha. A further increase in the number of plants per unit area led to a decrease in crop yields. The influence of four variants of the main application of mineral fertilizers on the productivity of St. John's wort was also studied: N0P0K0 (reference), N60P60K60, N120P120K120 and N180P180K180. It was found that with increasing fertilizer application rate, the yield of dry raw materials increased. The most favorable conditions for growth and development of plants of St. John's wort developed in the variant with the maximum rate of fertilizer application - N180P180K180, where the yield of dry raw materials in the first year was 3,31 t / ha, and in the second year – 4,15 t / ha, which exceeded the reference result (without fertilizers) by 0,61 t / ha and 0,84 t / ha, respectively.

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Productivity of common Saint-John's wort (Hypericum perforatum L.) by using transplant reproduction method in the conditions of drip irrigation

Pryvedenyuk¹,

Shatkovskyi

2021

LRaWM

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Radiation-induced modification of secondary metabolites contents in plants of Salvia officinalis L. and Silybum marianum L.

Pchelovska¹,

Litvinov²,

Shylina³

et al. 2020

Fakt. eksp. evol. org.

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Aim. Long-term studies of the effect of sublethal doses of pre-sowing low-LET ionizing radiation on seeds have demonstrated the effectiveness of radiation biotechnology to stimulate the accumulation of pharmaceutically valuable secondary metabolites in medicinal raw materials of a number of common medicinal plants in Ukraine. The aim of this research was to evaluate the effectiveness of radiation technology in the case of seeds of Salvia officinalis L. and Silybum marianum L. Methods. X-ray irradiation of seeds at doses up to 50 Gy, spectrophotometric analysis of water-ethanol extracts, fluorescence microscopy of leaf epidermis were used. Results. Doses of pre-sowing X-ray irradiation were selected which maximally stimulate both the increase of biomass of medicinal raw materials and the accumulation of secondary metabolites. In particular for Salvia officinalis L. doses are 5 Gy and 20 Gy, for Silybum marianum L. are 10 Gy and 20 Gy. Conclusions. It is recommended to use two stimulating doses at the same time, as the result of exposure depends essentially on the physiological status of the seeds, growing conditions, varietal characteristics. Increasing the content of flavonoids and phenols in medicinal raw materials has been shown to be a reliable and reproducible marker of stimulating of rosemary acid accumulation in Salvia officinalis leaves. Keywords: radiation-induced stimulation, Salvia officinalis, Silybum marianum, rosemary acid, silibinin.

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Evaluation of the influence of different doses of pre-sowing irradiation of seeds on the content of flavonoids in Hypericum perforatum L. medicinal raw material

Cited by 2 publications

References 5 publications

Productivity of common Saint-John's wort (Hypericum perforatum L.) by using transplant reproduction method in the conditions of drip irrigation

Productivity of common Saint-John's wort (Hypericum perforatum L.) by using transplant reproduction method in the conditions of drip irrigation

Radiation-induced modification of secondary metabolites contents in plants of Salvia officinalis L. and Silybum marianum L.

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