Evaluation of the in vitro antimicrobial activity of an ethanol extract of Brazilian classified propolis on strains of Staphylococcus aureus

Abstract

Staphylococcus aureus (S. aureus) is one of the most frequent causes of hospital acquired infections. With the increase in multiple drug resistant strains, natural products such as propolis are a stratagem for new product discovery. The aims of this study were: to determine the in vitro antimicrobial activity of an ethanol extract of propolis; to define the MIC50 and MIC90 (Minimal Inhibitory Concentration – MIC) against 210 strains of S. aureus; to characterize a crude sample of propolis and the respective ethanol extract as to the presence of predetermined chemical markers. The agar dilution method was used to define the MIC and the high performance liquid chromatography (HPLC) method was used to characterize the samples of propolis. MIC results ranged from 710 to 2,850 µg/mL. The MIC50 and MIC90 for the 210 strains as well as the individual analysis of American Type Culture Collection (ATCC) strains of Methicillin-susceptible Staphylococcus aureus (MSSA) and Methicillin-resistant Staphylococcus aureus (MRSA) were both 1,420 µg/mL. Based on the chromatographic analysis of the crude sample and ethanol extracted propolis, it was concluded that propolis was a mixture of the BRP (SP/MG) and BRP (PR) types. The results obtained confirm an antimicrobial activity in relation to the strains of the S. aureus tested.

Antifungal and Antibacterial Metabolites from a French Poplar Type Propolis

“…Therefore it would be interesting to develop some alternative extraction of propolis using a nontoxic solvent such as subcritical water. In addition, it should be noticed that, as an intrinsic polytherapy, propolis may also circumvent the development of drug resistance by bacteria [ 60 ].…”

Antibacterial Properties of Propolis

“…This diversity of the chemical composition gives propolis an additional advantage as an antibacterial agent. The combination of many active ingredients and their presence in various proportions prevents the bacterial resistance from occurring [33].…”

Impact of Biohybrid Magnetite Nanoparticles and Moroccan Propolis on Adherence of Methicillin Resistant Strains of Staphylococcus aureus

“…3,4-dihydroxy-5-prenyl-cinnamic acid; 3,5-diprenyl-4-hydroxy-cinnamic acid [19] Table 2 depicts the chemical composition of the hydro-alcoholic extract of propolis. Flavonoids (31.9%), diterpenes (21.5%), and phenolic acid esters (16.5%) were the main groups of compounds found in this extract.…”