Antifungal and Antibacterial Metabolites from a French Poplar Type Propolis

Abstract

During this study, the in vitro antifungal and antibacterial activities of different extracts (aqueous and organic) obtained from a French propolis batch were evaluated. Antifungal activity was evaluated by broth microdilution on three pathogenic strains: Candida albicans, C. glabrata, and Aspergillus fumigatus. Antibacterial activity was assayed using agar dilution method on 36 Gram-negative and Gram-positive strains including Staphylococcus aureus. Organic extracts showed a significant antifungal activity against C. albicans and C. glabrata (MIC80 between 16 and 31 µg/mL) but only a weak activity towards A. fumigatus (MIC80 = 250 µg/mL). DCM based extracts exhibited a selective Gram-positive antibacterial activity, especially against S. aureus (SA) and several of its methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains (MIC100 30–97 µg/mL). A new and active derivative of catechin was also identified whereas a synergistic antimicrobial effect was noticed during this study.

Insight on Propolis from Mediterranean Countries: Chemical Composition, Biological Activities and Application Fields

“…Caffeic acid (5), p-coumaric acid (6), ferulic acid (7) and isoferulic acid (14) were also detected in those propolis samples. The study of Chasset et al [42] was in concordance with [40,41] finding about the composition of French propolis, where they investigated propolis from different areas in France through high-performance thin-layer chromatography and mass spectrometry (HPTLC/MS). Regardless the collection area of propolis in France, phenolic acids and flavonoids predominated in the extracts.…”

“…[140,162,163] Benzyl caffeate, p-coumaric acid and pinocembrin were predominant in French propolis sample and showed a great effectiveness against tested pathogens exhibiting an important antibacterial and antifungal activities against Staphylococcus aureus; Escherichia coli, and Candida albicans. [164] More recently, Boisard et al [41] have found the same properties of aqueous and methanolic extracts of poplar propolis, where they found a selective Gram-positive antibacterial activity, in particular against Staphylococcus aureus and several of its methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains. [41] Moreover, Amoros et al [165] have shown that 80 % ethanol extract of propolis from Renne, France, possess an antiviral effect against Herpes simplex virus (HSV-1).…”

“…[164] More recently, Boisard et al [41] have found the same properties of aqueous and methanolic extracts of poplar propolis, where they found a selective Gram-positive antibacterial activity, in particular against Staphylococcus aureus and several of its methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains. [41] Moreover, Amoros et al [165] have shown that 80 % ethanol extract of propolis from Renne, France, possess an antiviral effect against Herpes simplex virus (HSV-1). The authors also investigate the anti-HSV-1 activity of the abundant flavonoids identified in French propolis comparing the results with those from the ethanol extract of crude propolis.…”

“…Data regarding the chemical composition of propolis from France are scarce. Boisard et al [40,41] declared that phenolic acids esters [benzyl caffeate (12) and prenyl caffeate (13)] and flavonoids [pinobanksin-3acetate (4), pinocembrin (3), chrysin (2), and galangin (9)] were the most abundant constituents of French propolis. Caffeic acid (5), p-coumaric acid (6), ferulic acid (7) and isoferulic acid (14) were also detected in those propolis samples.…”

Impact of Biohybrid Magnetite Nanoparticles and Moroccan Propolis on Adherence of Methicillin Resistant Strains of Staphylococcus aureus

“…As examples: Boisard et al [17] found that the flavonoids pinobanksin-3-acetate, pinocembrin, chrysin, and galangin did not show anti-MRSA activity, in contrast to the prenyl caffeate. Nevertheless, the activities of the extracts were higher than the isolated compounds, which evidence a synergetic activity among the compounds present in the extracts.…”