Evaluation of the IDEXX ProCyte Dx analyzer for dogs and cats compared to the Siemens ADVIA 2120 and manual differential

Goldmann, Fee; Bauer, Natali; Moritz, Andreas

doi:10.1007/s00580-012-1608-1

Cited by 19 publications

(86 citation statements)

References 13 publications

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“…The samples were analyzed both with IDEXX ProCyte Dx automated hematology instrument (software version 00‐33_51) and Siemens Advia 2120 (software version 5.3.1.‐MS), both using settings for cats. Methods for the ProCyte and Advia have been reported previously . Both instruments are flow cytometers that use laser light scatter from cells in the sample and staining of cells to identify various cell types.…”

Section: Methodsmentioning

confidence: 99%

“…ProCyte reports a 5‐part feline automated differential count (total neutrophils, lymphocytes, monocytes, eosinophils, and basophils). ProCyte uses a fluorescent polymethine stain, which binds to nucleic acids and cytoplasmic organelles in leukocytes . Side fluorescent light (SFL) reflects polymethine staining of leukocytes.…”

Section: Methodsmentioning

confidence: 99%

“…ProCyte is a hematology instrument with advanced technology developed for veterinary use. Previous validation studies have concluded it has good to excellent performance with veterinary blood samples . ProCyte methods have been described previously .…”

mentioning

confidence: 99%

“…Previous validation studies have concluded it has good to excellent performance with veterinary blood samples . ProCyte methods have been described previously . ProCyte uses similar to the same technology as the Sysmex XT200iV,b which also has been described …”

mentioning

confidence: 99%

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Feline Differential Leukocyte Count with ProCyte Dx: Frequency and Severity of a Neutrophil‐Lymphocyte Error and How to Avoid It

Tvedten

Andersson

Lilliehöök

2017

Veterinary Internal Medicne

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BackgroundErroneous neutrophil and lymphocyte counts from analysis of feline blood samples were transferred directly into the hospital information system from the ProCyte Dx hematology instrument in our after‐hours laboratory. Errors usually were not detected by the users.Hypothesis/ObjectivesTo quantify the frequency and severity of errors associated with the ProCyte Dx analyzer and to identify methods to avoid the errors.AnimalsOne‐hundred six EDTA blood samples routinely submitted from feline hospital patients were analyzed.MethodsProCyte differential leukocyte counts were compared to 2 reference methods: Advia 2120 hematology instrument and manual enumeration. Limits for unacceptable deviation from the reference methods were defined as 18 for % lymphocytes and 23 for % neutrophils.ResultsFourteen of 106 samples had unacceptable errors for both lymphocytes and neutrophils compared to both reference methods. Median % lymphocytes in those 14 samples were 11.2, 15.0, and 53.0% for Advia, manual, and ProCyte, respectively. Median % neutrophils were 85.4, 81.5, and 34.2% for Advia, manual, and ProCyte, respectively. All errors were avoided by rejecting automated ProCyte differential leukocyte results whenever the dot plot appeared clearly incorrect, but only 9 of these 14 samples had a ProCyte WBC distribution error flag.Conclusions and Clinical ImportanceResults reported by ProCyte had markedly falsely increased lymphocyte and decreased neutrophil counts in 13% of feline patient samples. Users must reject automated differential leukocyte count results when the WBC dot plot appears overtly incorrect. Rejection based only on ProCyte WBC error flag was insufficient.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Feline Differential Leukocyte Count with ProCyte Dx: Frequency and Severity of a Neutrophil‐Lymphocyte Error and How to Avoid It

Tvedten

Andersson

Lilliehöök

2017

Veterinary Internal Medicne

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“…On the Procyte Dx, the WBC scattergram separates the cell based on granularity ( x ‐axis) and fluorescence ( y ‐axis). The granularity corresponds to the complexity of the cytoplasm and the fluorescence assesses the amount of RNA present in the cytoplasm of the cells . In Pelger–Huët anomaly, there is only a defect of the nucleus lobation; otherwise, cells are mature and have a normal granular content and as expected, the scattergram is comparable to that of a healthy cat (Figure A, B).…”

Section: Discussionmentioning

confidence: 93%

Pelger–Huët Anomaly in a cat

Deshuillers

Raskin

Messick

2014

Veterinary Clinical Pathol

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A 14-year-old, spayed female Domestic Shorthair cat was referred to the Purdue University Veterinary Teaching Hospital (PUVTH) for iodine 131 treatment of hyperthyroidism. Upon arrival, a biochemistry profile and a CBC were performed. Approximately 50% of the neutrophils and all the eosinophils observed were hyposegmented with a mature, condensed chromatin pattern. Nuclei had a band to "dumbbell" shape, and rarely a round shape, suggesting a Pelger-Huët anomaly or a pseudo Pelger-Huët. Based on both a negative FeLV and FIV tests, the absence of any clinical signs to support an inflammatory process, and the persistence of this granulocytic morphology 6 months after its previous admission to the PUVTH, a diagnosis of Pelger-Huët anomaly was established in this cat.

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Reticulocyte and Heinz Body Staining and Enumeration

Tvedten¹,

Moritz²

2022

Schalm's Veterinary Hematology

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Evaluation of the IDEXX ProCyte Dx analyzer for dogs and cats compared to the Siemens ADVIA 2120 and manual differential

Cited by 19 publications

References 13 publications

Feline Differential Leukocyte Count with ProCyte Dx: Frequency and Severity of a Neutrophil‐Lymphocyte Error and How to Avoid It

Feline Differential Leukocyte Count with ProCyte Dx: Frequency and Severity of a Neutrophil‐Lymphocyte Error and How to Avoid It

Pelger–Huët Anomaly in a cat

Reticulocyte and Heinz Body Staining and Enumeration

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