Reticulocyte and Heinz Body Staining and Enumeration

Tvedten, Harold; Moritz, Andreas

doi:10.1002/9781119500537.ch23

Cited by 2 publications

(13 citation statements)

References 21 publications

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“…Concerning intra‐assay variability, despite the ProCyte Dx counting reticulocytes in absolute numbers and not in percentages, its precision was poor on samples with low reticulocyte percentages, similar to what was recorded for manual counts. Since, especially in manual counts, the reticulocyte percentage is used to calculate the number of reticulocytes, which, according to the current literature, allows identification of the regenerative response, this variability could potentially cause a shift in the classification from regenerative to non‐regenerative anaemia and affect clinical decisions 1,14 . However, the high variability at low reticulocyte percentage values was not unexpected, since CVs were similar to those reported with other automated haematological analysers 15,16 .…”

Section: Discussionmentioning

confidence: 70%

“…In the current study, however, the comparison with manual aggregate counts revealed a significant difference and a proportional bias, contrary to what was observed with our total reticulocyte count. Again, this may in part be explained by the subjective classification of aggregate or punctate reticulocytes, but it may also depend on the inclusion of some punctate reticulocytes in the automated reticulocyte counts, as hypothesised by other authors for both canine and feline samples 1,21,22 …”

Section: Discussionmentioning

confidence: 97%

“…This discrepancy may be due to the different criteria used to classify punctate or aggregate reticulocytes, which may be based only on the presence/absence of clumped dots, 13 as in the current study, or on the enumeration of intracytoplasmic dots. 1,3 The visual examination of intracytoplasmic dots is by definition subjective, and individual variability may have affected the relative proportion of aggregate versus punctate reticulocytes, especially in the samples with reticulocyte counts close to or lower than 1%.…”

Section: Discussionmentioning

confidence: 99%

“…Reticulocytosis is the most objective marker of bone marrow regenerative response for the erythrocyte lineage. 1 The magnitude of reticulocytosis allows the classification of anaemia as regenerative or nonregenerative, playing an important role in supporting clinical decisions, monitoring the efficacy of therapies and as a prognostic factor. 1 The distinction between reticulocytes and mature red blood cells is based on the presence of RNA and other particles (organelles, mitochondria) in reticulocytes, which are lost during differentiation into mature red blood cells.…”

Section: Introductionmentioning

confidence: 99%

“…1 The magnitude of reticulocytosis allows the classification of anaemia as regenerative or nonregenerative, playing an important role in supporting clinical decisions, monitoring the efficacy of therapies and as a prognostic factor. 1 The distinction between reticulocytes and mature red blood cells is based on the presence of RNA and other particles (organelles, mitochondria) in reticulocytes, which are lost during differentiation into mature red blood cells. 2 Manual counting of reticulocytes by light microscopy with supravital dyes for RNA was developed in the 1940s and has remained the standard method of reticulocyte enumeration for years, both in human 2 and veterinary medicine.…”

Section: Introductionmentioning

confidence: 99%

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Pre‐analytical and analytical variability of reticulocyte counts in dogs

Franchi

Giraldi²,

Bertazzolo³

et al. 2023

Veterinary Record

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Background: Automated fluorescence-based haematology analysers are now available for reticulocyte enumeration in veterinary medicine, but manual counting is still largely used. This study aimed to evaluate potential sources of analytical and pre-analytical errors when performing automated and manual counts. Methods: Automated and two-operator double-blind manual reticulocyte counts were performed on 15 blood samples. The intra-assay variation of the automated and manual counts and the interoperator variation in the manual counts were then calculated. In addition, the effects of storage were evaluated using samples refrigerated at 4 • C or stored at room temperature for 2, 4, 12, 24, 48 or 72 hours after sampling. Results: Intra-assay coefficients of variation were lower for automated counts than for manual counts. Comparison between automated and mean total manual reticulocyte count showed no significant differences. In both refrigerated samples and those stored at room temperature, an increase in reticulocyte count was recorded only after 72 hours. Staining artefacts occurred only in one stored sample counted manually. Limitations: The presence of cytoplasmic particles other than RNA can cause misinterpretation of cells, leading to an erroneous reticulocyte count. Conclusion:The use of an automated analyser is preferable for reticulocyte enumeration in dogs. Common storage conditions seem to minimally affect reticulocyte evaluation; however, it is recommended to perform the analysis as soon as possible after sampling.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Pre‐analytical and analytical variability of reticulocyte counts in dogs

Franchi

Giraldi²,

Bertazzolo³

et al. 2023

Veterinary Record

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Light microscopic morphology of blood cells of non‐descript indigenous Zoar chicken of Mizoram, India

Doley,

Sarma,

Kalita

et al. 2024

Anat Histol Embryol

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Identifying and analysing distinct blood cells is crucial for the diagnosis and treatment of diseases in the field of biomedicine. The present study was undertaken to study the cytomorphological and cytochemical characteristics of the blood cells of Zoar, a non‐descript indigenous breed of chicken extensively reared under backyard poultry farming in Mizoram, India. For this study, 2 mL of blood samples were aseptically collected from the wings veins of 12 chickens and were processed for light microscopic study under standard protocols. The matured erythrocytes were elliptical, while the immature erythrocytes appeared oval. The heterophils were positive for SBB (SBB), Periodic Acid Schiff (PAS), acid phosphatase, alkaline phosphatase and Arylsulphatase while the eosinophils were positive for SBB, PAS, alkaline phosphatase, cytochrome oxidase and peroxidase. The basophils of were positive for toluidine blue while the thrombocytes were positive for PAS. These cytochemical and cytoenzymatic staining properties plays a very important role in diagnosis, differentiation, and classification of leukaemias.

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Reticulocyte and Heinz Body Staining and Enumeration

Cited by 2 publications

References 21 publications

Pre‐analytical and analytical variability of reticulocyte counts in dogs

Pre‐analytical and analytical variability of reticulocyte counts in dogs

Light microscopic morphology of blood cells of non‐descript indigenous Zoar chicken of Mizoram, India

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