Evaluation of the <i>in vitro</i> cholesterol‐lowering activity of the probiotic strain <i>Bacillus coagulans</i><scp>MTCC</scp> 5856

Majeed, Muhammed; Majeed, Shaheen; Nagabhushanam, Kalyanam; Sivakumar, A.; Beede, Kirankumar; Ali, Furqan

doi:10.1111/ijfs.13926

Cited by 26 publications

(12 citation statements)

References 35 publications

(41 reference statements)

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“…The accumulation of cholesterol in the human leads to cardiovascular disorders resulting in life-threatening conditions [113]. The high serum cholesterol level can be considerably managed through cholesterol lowering drugs but ends with side effects.…”

Section: Hypocholestrolemic and Antidiabetic Activitymentioning

confidence: 99%

Exopolysaccharides from probiotic bacteria and their health potential

Angelin

Kavitha

2020

International Journal of Biological Macromolecules

366

225

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Exopolysaccharides (EPS) are extracellular macromolecules excreted as tightly bound capsule or loosely attached slime layer in microorganisms. They play most prominent role against desiccation, phagocytosis, cell recognition, phage attack, antibiotics or toxic compounds and osmotic stress. In the last few decades, natural polymers have gained much attention among scientific communities owing to their therapeutic potential. In particular the EPS retrieved from probiotic bacteria with varied carbohydrate compositions possess a plenty of beneficial properties. Different probiotic microbes have unique behavior in expressing their capability to display significant health promoting characteristics in the form of polysaccharides. In this new era of alternative medicines, these polysaccharides are considered as substitutes for synthetic drugs. The EPS finds applications in various fields like textiles, cosmetics, bioremediation, food and therapeutics. The present review is focused on sources, chemical composition, biosynthetic pathways of EPS and their biological potential. More attention has been given to the scientific investigations on antimicrobial, antitumor, anti-biofilm, antiviral, anti-inflammatory and immunomodulatory activities.

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Section: Hypocholestrolemic and Antidiabetic Activitymentioning

confidence: 99%

Exopolysaccharides from probiotic bacteria and their health potential

Angelin

Kavitha

2020

International Journal of Biological Macromolecules

366

225

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“…Isolate B11 showed 92 ± 0.11% removal with the lowest concentration (100 µg/ml) which was decreased to 72 ± 0.55% with 500 µg/ml cholesterol. In contrast, Majeed et al [28] observed regardless of minimum (25 µg/ml) or maximum (200 µg/ml) concentrations of cholesterol, Bacillus coagulans was able to remove nearly 50% of total cholesterol from the medium.…”

Section: Cholesterol Removal With Different Cholesterol Concentrationmentioning

confidence: 83%

In-vitro investigation of cholesterol removal, ß-galactosidase synthesis, antioxidant, and antidiabetic potential of probiotic organisms

Chanpura¹,

Gupte²

2022

J App Biol biotech

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The present study is aimed to determine some important health beneficial properties of probiotic isolates such as cholesterol removal, β-galactosidase production, antioxidant, and anti-diabetic activity. Nine probiotic isolates were screened for Bile Salt Hydrolase (BSH) activity and potential BSH producers were selected for cholesterol removal study. Isolate B11 has shown significant cholesterol removal ability (88 ± 0.2%) after 24 h and this property was also analyzed with variable concentrations, time profile, with live, and dead cells as well as from egg yolk with simulation treatment. As probiotics improve lactose intolerance, all isolates were assessed for their lactose utilizing and β-galactosidase production ability where maximum enzyme activity was observed in case of isolate D25 (226 ± 0.30 Miller units). All the isolates were also assessed for their antioxidant potential using four different methods such as reducing power assay, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, 2,2-diphenyl-1-picrylhydrazyl assay, and superoxide dismutase activity. Out of nine isolates, isolate D25 and B11 have shown significant antioxidant property. Further, these isolates were also subjected to anti-diabetic study, where isolate D25 (89 ± 0.01%) and B11 (98 ± 0.30%) have shown high α-glucosidase inhibition which indicate their effective anti-diabetic activity. Hence, overall probiotic isolate D25 and B11 have shown significant health beneficial properties and they were further identified as Lactiplantibacillus plantarum and Pediococcus pentosaceus, respectively.

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“…The relative abundance of Collinsella aerofaciens and Bacillus coagulans was significantly and positively correlated with butyric acid production ( p < 0.05). Collinsella aerofaciens is a novel butyrate producer isolated from a human gut [ 49 ], and Bacillus coagulans enhanced the production of butyric acid in cholesterol-rich foods [ 50 ]. Oscillospira , Turicibacter and Ruminococcus were negatively correlated with SCFA production ( p < 0.05), reflecting the suppressive effect of these bacteria on SCFA production.…”

Section: Resultsmentioning

confidence: 99%

Impact of Co-Delivery of EGCG and Tuna Oil within a Broccoli Matrix on Human Gut Microbiota, Phenolic Metabolites and Short Chain Fatty Acids In Vitro

et al. 2022

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(-)-Epigallocatechin gallate (EGCG) and tuna oil (TO) are beneficial bioactive compounds. EGCG, TO or a combination of, delivered by broccoli by-products (BBP), were added to an in vitro anaerobic fermentation system containing human fecal inocula to examine their ability to generate short-chain fatty acids (SCFA), metabolize EGCG and change the gut microbiota population (assessed by 16 S gene sequencing). Following 24 h fermentation, EGCG was hydrolyzed to (-)-epigallocatechin and gallic acid. EGCG significantly inhibited the production of SCFA (p < 0.05). Total SCFA in facal slurries with BBP or TO-BBP (48–49 µmol/mL) were significantly higher (p < 0.05) than the negative control with cellulose (21 µmol/mL). EGCG-BBP and TO-EGCG-BBP treatment increased the relative abundance of Gluconacetobacter, Klebsiella and Trabulsiella. BBP and TO-BBP showed the greatest potential for improving gut health with the growth promotion of high butyrate producers, including Collinsella aerofaciens, Bacillus coagulans and Lactobacillus reuteri.

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Evaluation of the in vitro cholesterol‐lowering activity of the probiotic strain Bacillus coagulansMTCC 5856

Cited by 26 publications

References 35 publications

Exopolysaccharides from probiotic bacteria and their health potential

Exopolysaccharides from probiotic bacteria and their health potential

In-vitro investigation of cholesterol removal, ß-galactosidase synthesis, antioxidant, and antidiabetic potential of probiotic organisms

Impact of Co-Delivery of EGCG and Tuna Oil within a Broccoli Matrix on Human Gut Microbiota, Phenolic Metabolites and Short Chain Fatty Acids In Vitro

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