Evaluation of the Humoral Immune Response in Trachoma to Chlamydia trachomatis Major Outer Membrane Proteins by Sequence-Defined Immunoassay

Jones, Heath; Schachter, Julius; Stephens, Richard S.

doi:10.1093/infdis/166.4.915

Cited by 16 publications

(12 citation statements)

References 12 publications

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“…The outer surface of Chlamydiae contains two major antigens, LPS and the major outer membrane protein (MOMP). During the course of natural and experimental infection, both antigens interact with the immune system to induce antibodies directed against MOMP 22 and LPS 23 in the sera of infected patients. Recently, a second protein has , and 2 Â 10 7 IFU) of (a) C pneumoniae (TW183) and (b) C trachomatis (UW-5) for 24 h at 371C.…”

Section: Resultsmentioning

confidence: 99%

A CD14 promoter polymorphism is associated with CD14 expression and Chlamydia-stimulated TNFα production

Eng¹,

Wang²,

Chen³

et al. 2004

Genes Immun

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CD14, a pattern recognition receptor on monocyte and macrophage, plays a central role in innate immunity through recognition of bacterial lipopolysaccharide and initiation of inflammatory response. Recently, CD14/À260C4T promoter gene polymorphism has been found to be related to a risk of inflammatory diseases. Our results showed that the C allele frequency among Chinese in Taiwan was lower than those in Western countries. The membrane CD14 expression was significantly higher in TT as compared with CT and CC genotypes (P ¼ 0.034, 0.044, respectively). There was a higher level of soluble CD14 in TT and CT genotypes than in CC genotypes. In addition, TNFa production in whole blood was significantly higher in TT genotype than in CC genotype after stimulation by Chlamydiae. In conclusion, the single base pair polymorphism of CD14 promoter gene is associated with CD14 expression and Chlamydia-stimulated TNFa production, and may thus play some role in the chlamydia-induced inflammatory response.

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Section: Resultsmentioning

confidence: 99%

A CD14 promoter polymorphism is associated with CD14 expression and Chlamydia-stimulated TNFα production

Eng¹,

Wang²,

Chen³

et al. 2004

Genes Immun

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“…2). In addition to the upstream Nco I site and a downstream Bam HI site, an Apa I restriction endonuclease site was introduced downstream of the momp encoding sequence, to facilitate subsequent cloning of the fusion construct (Table 1 recognised by antibodies in sera from trachoma patients [21].) This was done by synthetically producing a set of oligonucleotides encoding both momp fragments (Table 1, oligonuclotide set 2).…”

Section: P Mygind Et Almentioning

confidence: 99%

“…It is considered to be a porin, spanning the outer membrane of all Chlamydia species. Infections with C. trachomatis induce a strong humoral response mainly directed against three variable, surface-exposed segments of this protein (VS-I, VS-II and VS-IV) [20,21]. These regions are also the basis of the C. trachomatis serovar typing.…”

Section: Introductionmentioning

confidence: 99%

Detection of Chlamydia trachomatis-specific antibodies in human sera by recombinant major outer-membrane protein polyantigens

Mygind¹,

Christiansen

Persson³

et al. 2000

Journal of Medical Microbiology

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This study was performed to generate and evaluate recombinant antigens for use in a species-speci®c Chlamydia trachomatis immunoassay. In a molecular genetic approach, fragments of the C. trachomatis major outer-membrane protein (MOMP) were produced as fusion proteins to create three different constructs encompassing the variable domains I, II and IV of selected C. trachomatis serovars. The recombinant MOMP polyantigens were af®nity-puri®ed and used in an enzyme-linked immunosorbent assay. Antibody detection was evaluated with 103 patient sera and the results were compared with titres obtained in the micro-immuno¯uorescence test. The results showed that the generated MOMP polyantigens detected the presence of C. trachomatis-speci®c human antibodies with little cross-reaction to C. pneumoniae-speci®c antibodies. When compared to the micro-immuno¯uorescence assay the MOMP polyantigen detected the presence of anti-C. trachomatis IgG antibodies with a sensitivity of 80% and a speci®city of 91%.

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“…Heterogeneous antigens do not allow the determination of distinct antibody specificities, and Western blots, using whole cell lysates of EBs, separated by SDS-PAGE [11] are not useful in a routine laboratory and lack sensitivity. Recently, an ELISA has been described to measure antibodies against defined epitopes of the chlamydial major outer membrane protein [12]. This assay used, as a solid phase antigen, synthetic peptides representing serovar-specific sequences of variable segment 1 and 2 of the major outer membrane protein [13,14].…”

Section: Introductionmentioning

confidence: 99%

Occurence of antibodies against chlamydial lipopolysaccharide in human sera as measured by ELISA using an artificial glycoconjugate antigen

Brade¹,

Brunnemann²,

Ernst³

et al. 1994

FEMS Immunology &Amp; Medical Microbiology

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An artificial glycoconjugate containing, as a ligand, the deacylated carbohydrate backbone of a recombinant Chlamydia-specific lipopolysaccharide was used as a solid-phase antigen in ELISA to measure antibodies against chlamydial LPS. The specificity and reproducibility of the assay was shown by using a panel of prototype monoclonal antibodies representing the spectrum of antibodies also occurring in patient sera. These mAbs recognized Chlamydia-specific epitopes [alpha 2-->8-linked disaccharide of 3-deoxy-D-manno-octulosonic acid (Kdo) or the trisaccharide alpha Kdo-(2-->8)-alpha Kdo-(2-->4)-alpha Kdo] or those shared between chlamydial and Re-type LPS (alpha Kdo, alpha 2-->4-linked Kdo disaccharide). The assay was used to measure IgG, IgA and IgM antibodies against chlamydial LPS in patients with genital or respiratory tract infections. In comparison to the results obtained with sera from blood donors, it became evident that both types of infection result in significant changes in the profile of LPS antibodies.

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Evaluation of the Humoral Immune Response in Trachoma to Chlamydia trachomatis Major Outer Membrane Proteins by Sequence-Defined Immunoassay

Cited by 16 publications

References 12 publications

A CD14 promoter polymorphism is associated with CD14 expression and Chlamydia-stimulated TNFα production

A CD14 promoter polymorphism is associated with CD14 expression and Chlamydia-stimulated TNFα production

Detection of Chlamydia trachomatis-specific antibodies in human sera by recombinant major outer-membrane protein polyantigens

Occurence of antibodies against chlamydial lipopolysaccharide in human sera as measured by ELISA using an artificial glycoconjugate antigen

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