A CD14 promoter polymorphism is associated with CD14 expression and Chlamydia-stimulated TNFα production

Abstract

CD14, a pattern recognition receptor on monocyte and macrophage, plays a central role in innate immunity through recognition of bacterial lipopolysaccharide and initiation of inflammatory response. Recently, CD14/À260C4T promoter gene polymorphism has been found to be related to a risk of inflammatory diseases. Our results showed that the C allele frequency among Chinese in Taiwan was lower than those in Western countries. The membrane CD14 expression was significantly higher in TT as compared with CT and CC genotypes (P ¼ 0.034, 0.044, respectively). There was a higher level of soluble CD14 in TT and CT genotypes than in CC genotypes. In addition, TNFa production in whole blood was significantly higher in TT genotype than in CC genotype after stimulation by Chlamydiae. In conclusion, the single base pair polymorphism of CD14 promoter gene is associated with CD14 expression and Chlamydia-stimulated TNFa production, and may thus play some role in the chlamydia-induced inflammatory response.

Aberrant TNF secretion by whole blood in healthy subjects with a history of reactive arthritis: time course in adherent and non-adherent cultures

“…In agreement with a previous study, 31 the CD14(2159)T allele increased LPS induced TNF production in an allele dose dependent manner. However, the frequency of CD14(2159)T and the proportion of CD14+CD16+ monocytes in whole blood samples were comparable in the subject groups.…”

Soluble CD14 in periodontitis

“…14,32 Albeit not conclusive, there are indications for the association of the CD14 T/T genotype with severe periodontal disease. 16 In our study, the CD14 À260 genotypes were present with similar frequency in patients and controls.…”

Polymorphisms in the novel gene acyloxyacyl hydroxylase (AOAH) are associated with asthma and associated phenotypes

“…4,6,[52][53][54][55] Soluble CD14 has been shown to be correlated with development of recurrent wheezing in the first year of life and other markers of atopy, such as IFN-g. 56 Previously, we did not observe any association between sCD14 concentrations and CD14(2260)C>T 29 ; however, when we tested for association between variants in AOAH and sCD14 concentration, we found several AOAH variants/haplotypes were associated with low sCD14 levels. We also observed a significant association between a marker of the T H 2 phenotype (IL-13/IFN-g ratio) and several AOAH markers, suggesting that the AOAH gene plays a role in the T H 2 milieu in addition to its effect on sCD14 levels.…”