Evaluation of The Genotoxicity of Three Food Additives using CHO-K1 Cells under in vitro Micronucleus Flow Cytometry Assay

Lestari, Beni; Novitasari, Dhania; Putri, Herwandhani; Haryanti, Sari; Sasmito, Ediati; Meiyanto, Edy

doi:10.14499/indonesianjcanchemoprev8iss2pp74-80

Cited by 4 publications

(2 citation statements)

References 14 publications

(11 reference statements)

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“…Reactive oxygen species (ROS) like H 2 O 2 and O 2 -cause a series of DNA lesions, including single-strand, double-strand breaks, cross-linking of DNA and damage to bases. The conversion of these ROS to highly reactive OH-radical is responsible for DNA damage 4 . Natural antioxidants such as vitamin C, vitamin E, flavonoids, and phenolic acids fight against free radical species that cause DNA damages.…”

Section: Quick Response Codementioning

confidence: 99%

Untitled

2020

IJPSR

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Many studies have demonstrated the antioxidant properties of extracts of Coleus aromaticus. The purified Coleus aromaticus leaves protein was studied to investigate its in-vitro antioxidant activity, preventing oxidative DNA damage induced by hydrogen peroxide (H 2 O 2) and its protective effect on lymphocyte cell viability. Materials and Methods: In-vitro antioxidant capacity by DPPH assay, hydrogen peroxide (H 2 O 2) used to damage the calf thymus DNA, the purified Coleus aromaticus leaves protein (CALP) at 25 μg concentration was used to prevent the H 2 O 2 induced DNA damage, which was demonstrated on submarine agarose gel electrophoresis. The protective effect of purified CALP on cell viability of human peripheral lymphocytes against damage induced by H 2 O 2 was studied by the Trypan dye blue exclusion method. Standard antioxidants like gallic acid, ascorbic acid, and butylated hydroxyanisole were used. Results: The purified CALP protein showed significant antioxidant activity when compared to Gallic acid. At 25 μg concentration of purified CALP provided almost equal protection as given by standard antioxidant Ascorbic acid at 400 μM concentrations against H 2 O 2 induced DNA fragmentation in submarine agarose gel electrophoresis. The cell viability study showed that human peripheral lymphocytes treated with the purified CALP (25 µg) had 71 ± 2% viability, BHA (400 μM) had 68 ± 3% viability, and Ascorbic acid (400 µM) had 78 ± 2% viability when compared to untreated lymphocytes (83 ± 4%). Conclusion: The study demonstrated that the purified CALP possesses significant antioxidant activity, protective effect on H 2 O 2 induced DNA damage, and lymphocyte cell damage, which showed itself is not toxic.

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Section: Quick Response Codementioning

confidence: 99%

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2020

IJPSR

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“…Previous studies demonstrated that artificial food flavoring was able to induce chromosomal aberrations in mice bone marrow cells, Vicia faba, Allium cepa and Chinese hamsters' cells (Matsuoka et al 1979, Pandey and Upadhyay 2007, Tripathy and Rao 2015, Hassan and Jasem, 2020. several other studies assayed the genotoxicity of food flavorings us-ing micronucleus assay in human lymphocytes, Chinese Hamster Ovary-K1 (CHO-K1) cells, Fetal lung fibroblast cells (MRC-5), normal human corneal epithelial cells (HCEC) and immortalized human keratinocytes cells (HaCaT) (Mamur et al 2012, Lestari et al 2017, Banti et al 2019.…”

Section: Introductionmentioning

confidence: 99%

Diacetyl and Butter Flavors Induces Genotoxic Effects and Liver Toxicity in Male Swiss Albino Mice after Acute and Chronic Exposure.

Aboelmaaty

Fahmy

Ebeed

et al. 2021

Arab Universities Journal of Agricultural Sciences

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Since the mid-1950s, the volatile structure of butter oil and butter were researched, and an exhaustive list of elements has been collected. Diacetyl is an aromatic popular synthetic fragrance that gives food a buttery taste used in ice cream, snacks and potting with butter, strawberry, caramel, or cheese flavor. The chromosomal aberrations and micronuclei are commonly used biomarkers of chromosomal damage, genome stability, and cancer risk assessment. In vivo trials are still important to assess the genetic toxicology of chemical products such as industrial chemicals, pharmaceuticals, and food additives. This study aimed at assessing the potential genotoxic effect of diacetyl and butter flavors on swiss albino mice using alterations in liver function enzymes, micronucleus (MN), and chromosomal aberrations (CA) assays. The results showed that exposure of swiss albino mice males to diacetyl and butter flavors induced (CA) and (MN) in a statistically highly significant manner compared to the control. Meanwhile, the biochemical analysis revealed that these substances caused an exceptional rise in liver function enzymes (AST, ALT, and ALP) activity in serum of treated experimental animals. In conclusion, both tested compounds have increased the chromosomal aberration, micronucleus test, and serum levels of liver function enzymes indicating their high potential of being cytotoxic and genotoxic materials.

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Therapeutic effects of royal jelly against sodium benzoate–induced toxicity: cytotoxic, genotoxic, and biochemical assessment

Acar

2021

Environ Sci Pollut Res

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Evaluation of The Genotoxicity of Three Food Additives using CHO-K1 Cells under in vitro Micronucleus Flow Cytometry Assay

Cited by 4 publications

References 14 publications

Untitled

Untitled

Diacetyl and Butter Flavors Induces Genotoxic Effects and Liver Toxicity in Male Swiss Albino Mice after Acute and Chronic Exposure.

Therapeutic effects of royal jelly against sodium benzoate–induced toxicity: cytotoxic, genotoxic, and biochemical assessment

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