Evaluation of the genotoxic potential of Austroplenckia populnea (Reiss) Lundell chloroform fraction from barkwood extract in rodent cells in vivo

Ribeiro, Jc.; Andrade, Sf.; Bastos, Jairo K.; Maistro, Edson Luis

doi:10.1590/s1519-69842009000500019

Cited by 5 publications

(4 citation statements)

References 22 publications

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“…Recently, using the micronucleus test in mice bone marrow, our research team revealed that the three fractions of S. crassifolia studied in the present work (HE, EA and HA), did not cause significant increase in the frequency of micronucleated polychromatic erythrocytes compared to the solvent control group (Carneiro et al, 2013). Flammang et al (2006) and Ribeiro et al (2009) also did not found any statistically significant induction in micronucleated polychromatic erythrocytes of mice bone marrow treated with root extract of S. oblonga and bark extract of Austroplenckia populnea (Reiss) Lundell (Celastraceae), respectively. In culture of peripheral blood lymphocytes from rat, any induction of chromosomal aberrations was not found after 90 consecutive days of treatment with root extract of S. oblonga (Flammang et al, 2007).…”

Section: Resultsmentioning

confidence: 45%

Mutagenicity and antimutagenicity of Salacia crassifolia (mart. Ex. Schult.) G. Don. evaluated by Ames test

et al. 2017

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Salacia crassifolia (Mart. Ex. Schult.) G. Don. is a bush which belongs to Celastraceae family and occurs specially in Brazilian Cerrado. Its leaves, stem, seeds and fruits are popularly used for several medicinal purposes, such as antitumoral, antirheumatic, anti-inflammatory and antimicrobial. In this study, the mutagenic and antimutagenic activities of S. crassifolia stem bark fractions (hexane, ethyl acetate and hydroalcoholic) were evaluated by the Ames mutagenicity assay in Salmonella typhimurium TA98 and TA100 strains. By the obtained results, all S. crassifolia fractions did not significantly increase the number of prototrophic revertants for histidine (His + ) in both S. typhimurium strains tested (p > 0.05), suggesting absence of mutagenicity. Regarding antimutagenicity, the fractions ethyl acetate and hydroalcoholic significantly decreased the number of His + revertants colonies induced by positive control for strain TA98 (p < 0.05), demonstrating protection against mutagenicity induced by 4-nitroquinolile1-oxide, whereas the hexane fraction did not show antimutagenic effect in this strain. In the TA100 strain, all fractions of S. crassifolia protected DNA against the harmful action of sodium azide, and the hexane fraction exhibited the greatest protection in this work. Thus, it's possible conclude that the fractions of S. crassifolia tested in this study could be used in chemoprevention.Keywords: Salacia crassifolia, stem bark fractions, ames test, absence of mutagenicity, antimutagenicity. + induzidas pelo controle positive para a cepa TA98 (p < 0.05), demonstrando sua ação protetora contra a mutagenicidade induzida por 4-nitroquinolile1-oxide, enquanto a fração hexânica não demonstrou efeito antimutagênico nesta cepa. Na cepa TA100, todas as frações de S. crassifolia protegeram o DNA contra a ação lesiva de azida sódica, e a fração hexânica exibiu a maior proteção desse trabalho. Assim, concluímos que as frações de S. crassifolia testadas neste estudo poderiam ser utilizadas em quimioprevenção. Mutagenicitade e antimutagenicidade dePalavras-chave: Salacia crassifólia, frações da casca do caule, teste de ames, ausência de mutagenicidade, antimutagenicidade.

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Section: Resultsmentioning

confidence: 45%

Mutagenicity and antimutagenicity of Salacia crassifolia (mart. Ex. Schult.) G. Don. evaluated by Ames test

et al. 2017

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“…A. populnea hydroalcoholic leaf extract evaluated using the micronucleus test and chromosome aberration assays induced no clastogenic effects in the bone marrow cells of Wistar rats (Pugliesi et al, 2007). A chloroform fraction of barkwood extract induces an increase (although not a statistically significant one) in the average amount of DNA damage in peripheral blood cells of Swiss mice but also showed no clastogenic effects in the bone marrow and peripheral blood cells of the rodents (Ribeiro et al, 2009).…”

Section: Resultsmentioning

confidence: 77%

Genotoxicity of the medicinal plant Maytenus robusta in mammalian cells in vivo

Raymundo¹,

Favilla²,

Niero³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Plants belonging to the Celastraceae family have been used in traditional medicine for their analgesic, anti-inflammatory and anti-ulcerogenic properties, among others. Maytenus ilicifolia is the principal species of this family, and is used in the treatment of gastric ulcers. However, owing to its inadequate management in Brazil, the species is becoming extinct and is being substituted with Maytenus robusta, which also displays gastroprotective activity. The aim of this study was to evaluate the genotoxic effects of M. robusta hydroalcoholic extract in vivo, using the comet assay and micronucleus test. Three doses (50, 250 and 500 mg/kg body weight) were administered to mice orally 2 times at 24-h intervals. Cytotoxicity was assessed by scoring 200 consecutive total polychromatic and normochromatic erythrocytes to calculate their ratio. Parametric (analysis of variance/Tukey) and non-parametric (Kruskal-Wallis/Dunn post hoc) tests were used to evaluate the results according to the nature of the data distribution. The results showed a significant increase in the frequency of DNA damage on leukocytes at the 2 higher doses tested, but the extract did not enhance micronucleus frequency in bone marrow cells. Our findings showed that after 48 h of treatment, M. robusta hydroalcoholic extract had weak genotoxic effects but no clastogenic effects in mice cells.

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“…Plant extracts do not have obvious ways of action. Ribeiro et al (2009) showed that although the chloroform fraction of A. populnea (Reiss) Lundell shows no genotoxic or clastogenic/aneugenic effects in the bone marrow cells of Wistar rats, its crude bark wood extract shows a slight but significant increase in clastogenicity in those cells. On the other hand, Nikaidou et al (2005) showed that the catechins, important constituents of green tea, are able to reduce the mutagenic potential of hydroxyl radical.…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity investigation of araticum(Annona crassiflora Mart., 1841, Annonaceae) using SOS-Inductest and Ames test

2011

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Although the use of medicinal plants or natural products has increased in recent decades all over the world, little information is available on their potential risk to health. Annona crassiflora Mart., a plant commonly known as araticum in Brazil, has been widely used in folk medicine for a long time since its seeds and leaves are often utilised in the treatment of cancer, snake bites, and venereal diseases, its fruits are consumed as tonic and astringent, and its bark powder has anti-fungal and anti-rheumatic properties. To evaluate the genotoxic and mutagenic properties induced by the ethanolic extract of araticum leaves, we performed the prophage λ induction (Inductest) and bacterial mutagenicity assays. We used Escherichia coli WP2s(λ) and RJF013 strains in the lysogenic induction test, whereas the mutagenic studies were carried out using Salmonella typhimurium histidine auxotroph strains TA97a, TA98, TA100, and TA102. Each experiment was performed three times in duplicate and included positive and negative controls. No statistically significant (p > 0.05) positive results were obtained for any of the strains tested, which suggests that the ethanolic extract of araticum leaves did not exhibit direct mechanisms of genotoxicity or mutagenicity that could be detected by the tests used in the present work.

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Evaluation of the genotoxic potential of Austroplenckia populnea (Reiss) Lundell chloroform fraction from barkwood extract in rodent cells in vivo

Cited by 5 publications

References 22 publications

Mutagenicity and antimutagenicity of Salacia crassifolia (mart. Ex. Schult.) G. Don. evaluated by Ames test

Mutagenicity and antimutagenicity of Salacia crassifolia (mart. Ex. Schult.) G. Don. evaluated by Ames test

Genotoxicity of the medicinal plant Maytenus robusta in mammalian cells in vivo

Genotoxicity investigation of araticum(Annona crassiflora Mart., 1841, Annonaceae) using SOS-Inductest and Ames test

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