Evaluation of the food grade expression systems NICE and pSIP for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum

Abstract

2,5-diketo-D-gluconic acid reductase (2,5-DKG reductase) catalyses the reduction of 2,5-diketo-D-gluconic acid (2,5-DKG) to 2-keto-L-gulonic acid (2-KLG), a direct precursor (lactone) of L-ascorbic acid (vitamin C). This reaction is an essential step in the biocatalytic production of the food supplement vitamin C from D-glucose or D-gluconic acid. As 2,5-DKG reductase is usually produced recombinantly, it is of interest to establish an efficient process for 2,5-DKG reductase production that also satisfies food safety requirements. In the present study, three recently described food grade variants of the Lactobacillales based expression systems pSIP (Lactobacillus plantarum) and NICE (Lactococcus lactis) were evaluated with regard to their effictiveness to produce 2,5-DKG reductase from Corynebacterium glutamicum. Our results indicate that both systems are suitable for 2,5-DKG reductase expression. Maximum production yields were obtained with Lb. plantarum/pSIP609 by pH control at 6.5. With 262 U per litre of broth, this represents the highest heterologous expression level so far reported for 2,5-DKG reductase from C. glutamicum. Accordingly, Lb. plantarum/pSIP609 might be an interesting alternative to Escherichia coli expression systems for industrial 2,5-DKG reductase production.

Polyol production during heterofermentative growth of the plant isolate Lactobacillus florum 2F

“…; Kaswurm et al . ). However, because similar proteins were also predicted to be encoded by strains of Lact.…”

Comparative Assessment of Some Physicochemical Properties of Different Sesame Varieties and Oil Yield, Ethiopia

“…The extracted oil was used for determinations of physico-chemical analysis of oils of sesame varieties. Saponification value was determined using AOAC (2000) [11]. The acid value was determined using Ronald (1991) method [11].…”

“…Saponification value was determined using AOAC (2000) [11]. The acid value was determined using Ronald (1991) method [11]. Whereas the refractive index was analyzed using the method [11,12].…”

“…The acid value was determined using Ronald (1991) method [11]. Whereas the refractive index was analyzed using the method [11,12]. The specific gravity and ester values of sesame varieties were determined [13,14] respectively.…”

Finding and Producing Probiotic Glycosylases for the Biocatalysis of Ginsenosides: A Mini Review

“…The use of transformed probiotic bacteria with recombinant plasmids might be an alternative approach to the E. coli system in the nutraceutical industry for several reasons: (i) probiotics are appropriate to produce food-grade expression of glycosylases; (ii) the price of commercially-available media ( i.e ., de Man, Rogosa and Sharpe media (MRS)) for probiotics is only three-fold more expensive than culture media for E. coli ; (iii) probiotic bacteria have been considered as GRAS in multiple food products already [51,52]. However, only a few cases using genetically-engineered probiotics have been applied to biotransformation of ginsenosides.…”