Evaluation of the Endothelial Cell Density and the Central Corneal Thickness in Pseudoexfoliation Syndrome and Pseudoexfoliation Glaucoma

Purpose: To compare the corneal endothelial cell density (ECD) before and after cataract surgery in patients with and without pseudoexfoliation syndrome (PEX). Methods: In this prospective study, we compared the ECD in 62 PEX patients with 62 patients without PEX (controls). The mean age was 78.3 AE 6.2 years and 77.2 AE 5.9 years, respectively. Patients were examined before and at 6 months, 1 year and 2 years after cataract surgery. The corneal endothelium was examined with confocal microscopy, and the ECD was counted both automatically and semi-manually. Nine patients in the PEX group (15%) and 11 patients in the control group (18%) were lost to follow-up in the 2-year period. Within the PEX and the control groups, we also compared the ECD between patients with and without glaucoma. Results: Before surgery, the ECD (semi-manual counting) was 2258 AE 342 cells/mm 2 in the PEX group and 2322 AE 321 cells/mm 2 in the control group (p = 0.29). There were no significant differences in postoperative ECD between these groups at any visit. After 2 years, the ECD had changed by À679 AE 337 cells/mm 2 and À704 AE 484 cells/mm 2 , respectively (p = 0.78). The preoperative ECD was lower in eyes with glaucoma compared to eyes without glaucoma, both within the PEX group (p = 0.05) and the control group (p = 0.03). After surgery, there were no differences between eyes with or without glaucoma. Conclusion: The ECD was not significantly different in PEX eyes compared with control eyes, neither before nor after cataract surgery. However, there seemed to be a lower ECD in eyes with glaucoma before surgery.

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“…; Tomaszewski et al. ). Suggested explanations for the possibly lower ECD in glaucoma eyes are cell loss due to increased IOP (Brooks et al.…”

Section: Discussionmentioning

confidence: 98%

Corneal endothelial cell loss following cataract surgery in patients with pseudoexfoliation syndrome: a 2‐year prospective comparative study

Kristianslund

Pathak

Østern

et al. 2019

Acta Ophthalmologica

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“…Результаты исследований про-демонстрировали, что для больных ПЭС/ПЭГ также характерно уменьшение ЦТР. Предпола-гают, что такая особенность роговицы при на-личии указанной патологии может быть связана с уменьшением плотности клеток стромы и поте-рей клеток эндотелия [9,25]. В проведённом нами исследовании, несмотря на то, что у пациентов с ПЭГ ЦТР была ниже, чем в группе контроля (537,8 ± 7,3 и 560,3 ± 5,9 мкм), различия были недостоверны (p = 0,09).…”

Section: Discussionunclassified

Pseudoexfoliative glaucoma and molecular genetic characteristics of vitamin D metabolism

Beletskaya

Станиславовна²,

Astakhov

et al. 2018

Ophthalmology Reports

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Purpose. To study the possible association of 25-hydroxyvitamin D level, and vitamin D receptor (VDR) gene polymorphisms (BsmI, ApaI, TaqI, FokI) with pseudoexfoliative glaucoma (PEG) clinical manifestations. Methods. We examined 160 subjects (72 males (45%), and 88 females (55%)) aged from 55 to 75 years, residents of St. Petersburg and Leningrad region. 122 patients with PEG were enrolled in the main study group, the control group comprised 38 subjects without PEG, primary open angle glaucoma (POUG) and pseudoexfoliation syndrome (PES). 25(OH)D serum levels were assessed by chemiluminescent immunoassay (CLIA) method. Detection of VDR gene allele polymorphisms (ApaI, BsmI, FokI, and TaqI) was carried out using polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) technique. Results. Patients with PEG had lower 25(OH)D serum levels compared to patients in the control group (39.3 ± 1.2 and 52.7 ± 2.1 nMol/l, respectively, p < 0.01). The prevalence of vitamin D deficiency was found to be higher among PEG patients than among healthy subjects (86.4% and 59.5%, respectively, p < 0.01). The prevalence of b allele (p < 0.001) and bb genotype (p < 0.001) (BsmI polymorphism), as well as of f allele and ff genotype (p < 0.05) (FokI polymorphism) in PEG patients were higher compared to healthy subjects. We found that the Fallele carriers (FokI polymorphism) had greater corneal thickness than the ff genotype carriers (547.3 ± 4.1 μm and 502.1 ± 25.8 μm, respectively, p < 0.01). It was revealed, that bb genotype, Bb genotype (BsmI polymorphism), and ff genotype (FokI polymorphism) were associated with the increased risk of PEG (OR = 8.2, CI 95%: 3.4-19.9; OR = 3.9, CI 95%: 1.7-9.0; OR = 2.3, CI 95%: 1.2-4.5, respectively). Conclusions. Results of this study for the first time ever showed the association between BsmI and FokI VDR gene polymorphisms and pseudoexfoliative glaucoma.

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“…Martone et al указали на единичный случай на-личия дендритических клеток и гиперрефлектив-ных межклеточных микровключений при ПЭС [23]. Несколько авторов отметили возможность визуализации ПЭМ на эндотелии роговицы при помощи конфокальной микроскопии [12,24,25].…”

Section: Discussionunclassified

“…В свою очередь, избыток ПЭМ и инфильтрация ден-дритическими клетками, возможно, играют роль в нейропатии, затрагивающей суббазальные нерв-ные сплетения. Ряд авторов сообщает о снижении плотности и увеличении извитости суббазальных нервных волокон при ПЭС [8,24,25]. Приведён-ные нами данные не продемонстрировали досто-верного снижения их плотности при ПЭС.…”

Section: Discussionunclassified

The influence of pseudoexfoliative syndrome on corneal morphology based on in vivo confocal microscopy

Потемкин

Витальевич²,

Varganova

et al. 2017

Ophthalmology Reports

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 Конфокальная микроскопия -современный метод исследования, позволяющий в режиме реального времени оценить неинвазивно in vivo структуру роговицы, лимба и конъюнктивы. Цель -оценить основные морфологические изменения роговицы, наблюдаемые при конфокальной микроскопии у пациентов с псевдоэксфолиативным синдромом (ПЭС). Материалы и методы. Был обследован 21 пациент. Основную группу составили 12 пациентов с ПЭС, группу контроля -9 пациентов без ПЭС. Результаты. У пациентов с ПЭС наблюдалось снижение плотности клеток эпителия и стромы роговицы, большое количество гиперрефлективных межклеточных микровключений и дендритических клеток (p < 0,05). Ключевые слова: конфокальная микроскопия; псевдоэксфолиативный синдром.  Confocal microscopy is a modern examination method, which provides in real-time mode a noninvasive in vivo imaging of the cornea, limb, and conjunctiva. Purpose. To evaluate main morphological changes observed by confocal microscopy in patients with pseudoexfoliation (PEX) syndrome. Methods. 21 patients were examined. 12 patients with PEX syndrome were enrolled as the main group, and 9 patients without PEX -as controls. Results. In patients with PEX, there was a decreased cell density in the epithelium and the stroma of the cornea, as well as a lot of hyperreflective intercellular microdeposits and dendritic cells (p < 0.05). THE INFLUENCE OF PSEUDOEXFOLIATIVE SYNDROME ON CORNEAL MORPHOLOGY BASED ON IN VIVO CONFOCAL MICROSCOPY Key words: confocal microscopy; pseudoexfoliation syndrome. ВВЕДЕНИЕПсевдоэксфолиативный синдром (ПЭС) -системное, ассоциированное с возрастом забо-левание, для которого характерна продукция и накопление внеклеточного амилоидподобного материала [3,[5][6][7]. ПЭС поражает множество тканей и органов, но именно офтальмологиче-ские проявления являются основой для поста-новки диагноза [3,4,6]. Это возможно благо-даря тому, что рутинного биомикроскопического исследования достаточно для обнаружения псев-доэксфолиативного материала (ПЭМ). Типичным

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Evaluation of the Endothelial Cell Density and the Central Corneal Thickness in Pseudoexfoliation Syndrome and Pseudoexfoliation Glaucoma

Cited by 41 publications

References 29 publications

Corneal endothelial cell loss following cataract surgery in patients with pseudoexfoliation syndrome: a 2‐year prospective comparative study

Corneal endothelial cell loss following cataract surgery in patients with pseudoexfoliation syndrome: a 2‐year prospective comparative study

Pseudoexfoliative glaucoma and molecular genetic characteristics of vitamin D metabolism

The influence of pseudoexfoliative syndrome on corneal morphology based on in vivo confocal microscopy

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