Evaluation of the Effects of Bismuth Subgallate on Wound Healing in Rats. Histological Findings

Abstract

Introduction Bismuth subgallate (BS) is a yellow and odorless powder that has hemostatic astringent properties. Some otorhinolaryngologists and dentists currently use this substance to enhance wound healing. Objective The objective of this study is to evaluate the effects of bismuth subgallate on wound healing, through the analysis of inflammatory process, collagen production, and angiogenesis. Method A standard wound was made on the back of 60 male Wistar rats, using a biopsy punch. We created two groups: the experimental group, which underwent daily application of 0.5mg BS over the entire wound, and the control group, which underwent daily application of sodium chloride 0.9%. We performed a qualitative evaluation of the tissue on the third, seventh, and fourteenth day. We assessed inflammatory markers using Hematoxylin and Eosin (HE) stain, used Picrosirius stain for collagen analysis, and immunohistochemistry was used for angiogenesis analysis through evaluation of smooth muscle proliferation. Results Statistically, we found no significant differences between groups regarding inflammatory response on the third (p = 1), seventh (p = 0.474), and fourteenth day (p = 0.303). Also, collagen type I and III production showed no statistical differences between groups on the third (p = 0.436), seventh (p = 0.853), and fourteenth day (p = 0.436) of analysis. Immunohistochemistry did not present differences on angiogenesis between experimental and control group on the third (p = 0.280), seventh (p = 0.971), and fourteenth day (p = 0.218). Conclusion BS does not promote significant changes in inflammatory response, collagen, and angiogenesis. Thus, it does not influence healing on skin wounds on rats.

Effects of low‐level laser therapy on the healing of surgically closed incisions and surgically created open wounds in dogs

“…We elected to perform flank ovariectomies so that identical skin incisions could be created on opposite sides of the body while sterilizing the patient. The open wounds in this study were small, surgically created wounds in healthy skin by using a punch biopsy, a model not previously reported in dogs but reported in rats and mice . Square/rectangular wounds contract differently from circular wounds and have been shown to contract more effectively .…”

Uso do subgalato de bismuto como agente hemostático e sua influência no reparo tecidual em cirurgias odontológicas

“…Estudos em animais demonstram que o SB não apresenta influência no reparo tecidual (Tramontina et al, 2002;Puia, Renou, Rey, Guglielmotti, & Bozzini, 2009;Kim et al, 2012;Lima, Sampaio, Seidel, Branco & Sobreiro, 2016;Dos Santos, Sampaio, de Moraes & de Lima 2016;Luzzi et al, 2018), seja em tecidos moles (Tramontina et al, 2002;Kim et al, 2012;Lima et al, 2016;Dos Santos et al, 2016;Luzzi et al ,2018), ou duros (Puia, Renou, Rey, Guglielmotti & Bozzini, 2009).…”

Randomized clinical trial comparing three local hemostatic agents for dental extractions in patients under chronic anticoagulant therapy - A comparative study

“…It behaves as a light astringent, precipitating vascular proteins that may obliterate the small capillary vessels. Previous studies in rats showed that BS does not interfere with the wound-healing process[ 19 20 ] or postextraction bone healing. [ 21 ] Good hemostasis has been achieved by using the drug as local hemostatic in tonsillectomy[ 22 ] and in dentistry as topical hemostatic agent at palatal donor sites.…”