Evaluation of the Effects of Culture Media and Light Sources on In Vitro Growth of Brassavola nodosa (L.) Lindl. Hybrid

Abstract

Culture medium and light are important factors that affect the process of in vitro propagation of plants. Particularly for orchids, diverse culture media have been evaluated for micropropagation of many species and hybrids. More recently, light-emitting diodes (LEDs) have become widely used in agriculture, including micropropagation commercial operations, resulting in increased production and reduced costs compared to traditional fluorescent lights. Brassavola nodosa (L.) Lindl. is an orchid, with great potential for commercialization as a potted flowering plant due to the beauty and fragrance of its inflorescences. In this study, we evaluated the effects of culture media (VW, MS, and ½ MS) and light sources (three LED sources and one fluorescent light source) on the micropropagation of B. nodosa orchids. VW medium resulted in the best growth and development of in vitro shoots compared to MS and ½ MS media. Light sources with lower intensity, such as LED-3 (80 μmol m−2 s−1 PPFD) resulted in the best plant performance in vitro, while LED-2 (1015 μmol m−2 s−1 PPFD) showed the best plant performance ex vitro. Rooting was obtained in vitro without the need for a rooting phase. Survival ex vitro was 100%, with the successful growth and development of in vitro-derived plantlets during acclimatization.

Impact of monochromatic lights on the in vitro development of Cattleya walkeriana and effects on acclimatization

“…Photosynthetic pigments play an important physiological role for plant growth and development, as they are light receptors, a vital energy source for photosynthesis and other metabolic processes. In addition, the biosynthesis of plant pigments is dependent on light (Taiz et al, 2017). The analyzed contents of chlorophylls and carotenoids were not significant for plants in vitro or in acclimatization (Table 4).…”

“…Plants absorb the entire length of the light spectrum. For carbon fixation, they use photosynthetically active radiation (PAR), which corresponds to the blue (400 to 500 nm) and red (greater than 600 nm) zones of the visible spectrum (Taiz et al, 2017). The other bands, such as green and yellow, are absorbed in smaller amounts and are involved in processes such as increasing the amount of secondary metabolites, flowering and improvements in plant nutrition (Al Murad et al, 2021).…”

Navigating the future: exploring technological advancements and emerging trends in the sustainable ornamental industry

“…This can be particularly useful for the production of holiday flowers, such as Cyclamen, chrysanthemums, poinsettias, gerberas, petunias, Easter lilies (Heo et al, 2003;Higuchi et al, 2012;Mills-Ibibofori, et al, 2019). However, in general, studies investigating the effects of LED light on flower crops typically vary in terms of the specific flower species (Mills-Ibibofori et al, 2019) being studied, the type and intensity of the LED light used, the duration and timing of the light treatments, and the specific growth or developmental parameters being measured (Craig and Runkle, 2013;Park and Runkle, 2016;Matysiak, 2021;Vendrame et al, 2022). These studies often aim to optimize plant growth and development, increase yield, improve flower quality and color, or reduce disease…”

Secondary metabolites, hormonal homeostasis, and antioxidant enzymes of Moringa oleifera in response to white or violet Light Emitting Diodes (LEDs) combined with cytokinins under tissue culture conditions

“…Along with its function in photosynthetic activities, light may also operate as an external regulator in a number of physiological and morphogenic processes that change the structure and phytochemical levels of the plant. Compared to conventional fluorescent lights, light-emitting diodes (LEDs) have lately gained popularity in agriculture, particularly in commercial micropropagation operations, leading to higher production and lower prices (Vendrame et al, 2022).…”