Evaluation of the Effectiveness of the Biopreparation in Combination with the Polymer γ-PGA for the Biodegradation of Petroleum Contaminants in Soil

Wojtowicz, Katarzyna; Steliga, Teresa; Kapusta, Piotr; Brzeszcz, Joanna; Skalski, Tomasz

doi:10.3390/ma15020400

Cited by 9 publications

(21 citation statements)

References 113 publications

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“…Our preliminary research results suggest that PGA has significant potential for use in this process. The high survival rate of bacteria with the addition of PGA, even at high hydrocarbon concentrations, was also evidenced in [ 55 ].…”

Section: Discussionmentioning

confidence: 81%

“…However, PGA has been used to good effect as a protective coating for bacteria in the bioremediation process of petroleum contaminated marine sediments [ 54 ]. Recently, a positive effect of inoculation of bacterial strains with PGA used for biodegradation of PH in contaminated soils has also been demonstrated [ 55 ]. However, there is a lack of information on the effect of PGA alone on various hydrocarbon fractions.…”

Section: Introductionmentioning

confidence: 99%

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Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application

Zając

Fabiańska

Jedrszczyk

et al. 2022

IJERPH

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To improve the environmental sustainability of cleanup activities of contaminated sites there is a need to develop technologies that minimize soil and habitat disturbances. Cleanup technologies, such as bioremediation, are based on biological products and processes, and they are important for the future of our planet. We studied the potential of γ-poly glutamic acid (PGA) as a natural component of biofilm produced by Bacillus sp. to be used for the decomposition of petroleum products, such as heavy naphtha (N), lubricating oil (O), and grease (G). The study aimed to assess the impact of the use of different concentrations of PGA on the degradation process of various fractions of petroleum hydrocarbons (PH) and its effect on bacterial population growth in harsh conditions of PH contamination. In laboratory conditions, four treatments of PGA with each of the petroleum products (N, O, and G) were tested: PGA0 (reference), PGA1 (1% PGA), PGA1B (1% PGA with Bacillus licheniformis), and PGA10 (10% PGA). After 7, 28, 56, and 112 days of the experiment, the percentage yield extraction, hydrocarbon mass loss, geochemical ratios, pH, electrical conductivity, and microorganisms survival were determined. We observed an increase in PH removal, reflected as a higher amount of extraction yield (growing with time and reaching about 11% in G) and loss of hydrocarbon mass (about 4% in O and G) in all treatments of the PGA compared to the reference. The positive degradation impact was intensive until around day 60. The PH removal stimulation by PGA was also reflected by changes in the values of geochemical ratios, which indicated that the highest rate of degradation was at the initial stage of the process. In general, for the stimulation of PH removal, using a lower (1%) concentration of PGA resulted in better performance than a higher concentration (10%). The PH removal facilitated by PGA is related to the anionic homopoliamid structure of the molecule and its action as a surfactant, which leads to the formation of micelles and the gradual release of PH absorbed in the zeolite carrier. Moreover, the protective properties of PGA against the extinction of bacteria under high concentrations of PH were identified. Generally, the γ-PGA biopolymer helps to degrade the hydrocarbon pollutants and stabilize the environment suitable for microbial degraders development.

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Section: Discussionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application

Zając

Fabiańska

Jedrszczyk

et al. 2022

IJERPH

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“…[ 49 , 50 ], Pseudomonas aeruginosa [ 50 ], Pseudomonas putida [ 51 ], Pseudomonas stutzeri [ 52 ], Rhodococcus sp. [ 53 ], Rhodococcus erythropolis [ 54 , 55 , 56 ], Sphingobium and Novosphingobium , Sphingomonas sp. [ 57 , 58 ], Sphingomonas yanoikuyae [ 59 ], Staphylococcus warneri and Bacillus pumilus [ 60 ], Stenotrophomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Oil-Contaminated Soil Remediation with Biodegradation by Autochthonous Microorganisms and Phytoremediation by Maize (Zea mays)

Wojtowicz,

Steliga,

Kapusta

et al. 2023

Molecules

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Biological methods are currently the most commonly used methods for removing hazardous substances from land. This research work focuses on the remediation of oil-contaminated land. The biodegradation of aliphatic hydrocarbons and PAHs as a result of inoculation with biopreparations B1 and B2 was investigated. Biopreparation B1 was developed on the basis of autochthonous bacteria, consisting of strains Dietzia sp. IN118, Gordonia sp. IN101, Mycolicibacterium frederiksbergense IN53, Rhodococcus erythropolis IN119, Rhodococcus globerulus IN113 and Raoultella sp. IN109, whereas biopreparation B2 was enriched with fungi, such as Aspergillus sydowii, Aspergillus versicolor, Candida sp., Cladosporium halotolerans, Penicillium chrysogenum. As a result of biodegradation tests conducted under ex situ conditions for soil inoculated with biopreparation B1, the concentrations of TPH and PAH were reduced by 31.85% and 27.41%, respectively. Soil inoculation with biopreparation B2 turned out to be more effective, as a result of which the concentration of TPH was reduced by 41.67% and PAH by 34.73%. Another issue was the phytoremediation of the pre-treated G6-3B2 soil with the use of Zea mays. The tests were carried out in three systems (system 1—soil G6-3B2 + Zea mays; system 2—soil G6-3B2 + biopreparation B2 + Zea mays; system 3—soil G6-3B2 + biopreparation B2 with γ-PGA + Zea mays) for 6 months. The highest degree of TPH and PAH reduction was obtained in system 3, amounting to 65.35% and 60.80%, respectively. The lowest phytoremediation efficiency was recorded in the non-inoculated system 1, where the concentration of TPH was reduced by 22.80% and PAH by 18.48%. Toxicological tests carried out using PhytotoxkitTM, OstracodtoxkitTM and Microtox® Solid Phase tests confirmed the effectiveness of remediation procedures and showed a correlation between the concentration of petroleum hydrocarbons in the soil and its toxicity. The results obtained during the research indicate the great potential of bioremediation practices with the use of microbial biopreparations and Zea mays in the treatment of soils contaminated with petroleum hydrocarbons.

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“…These pollutants get to the soil mainly as a result of oil extraction and processing in refineries, as well as any failures during fuel storage. Over-exploitation of oil fields, failures during the extraction, storage and transport of raw materials as well as military activities were the main causes of soil contamination with petroleum substances [1][2][3][4]. In Poland, the areas of the former bases and post-Soviet military training grounds are heavily contaminated, as well as the former crude oil exploitation areas, land near refineries, gas stations, repair workshops for car-railroad rolling stock, airports [3,[5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…Over-exploitation of oil fields, failures during the extraction, storage and transport of raw materials as well as military activities were the main causes of soil contamination with petroleum substances [1][2][3][4]. In Poland, the areas of the former bases and post-Soviet military training grounds are heavily contaminated, as well as the former crude oil exploitation areas, land near refineries, gas stations, repair workshops for car-railroad rolling stock, airports [3,[5][6][7][8][9]. A large share of soils is in the south-eastern part of the country, contaminated with petroleum substances, after the former extraction of crude oil [1].…”

Section: Introductionmentioning

confidence: 99%

Green Technology as a Way of Cleaning the Environment from Petroleum Substances in South-Eastern Poland

Sawicka¹,

Vambol²,

Krochmal‐Marczak³

et al. 2022

Front. Biosci. (Elite Ed)

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Background: In phytoextraction methods, the problem is the obtained contaminated plant biomass, the selection of the appropriate species, resistant to the type and degree of contamination, as well as the long time needed to completely clean the soil. Goal: when selecting the appropriate method of remediation of soils contaminated with polycyclic aromatic hydrocarbons, not only the effectiveness of the method should be considered, but also the degree of contamination, the location of the site and its current and planned use. Methods: Descriptive, laboratory and comparative methods were used. Results: Soil contamination with polycyclic aromatic hydrocarbons (PAHs), which can cause mutations and cancer, is of particular concern as it affects not only human health but also vegetation growth and the biological environment. A fast, nature-friendly and cost-effective method is required to remove and minimize the hazardous effects of crude oil. Conclusions: Green technology is particularly beneficial, especially the phytoextraction technique, in which plants clean the soil of excess petroleum products, prevent its further movement from the site of contamination and prevent erosion of reclaimed soil. Species such as: Trifolium repens, Trifolium pratense, Lotus corniculatus, Agrostis stolonifera, Festuca rubra subsp. trichophylla, Arrhenatherum elatius performed their tasks very well, therefore they can be recommended for use as a factor counteracting environmental degradation.

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Evaluation of the Effectiveness of the Biopreparation in Combination with the Polymer γ-PGA for the Biodegradation of Petroleum Contaminants in Soil

Cited by 9 publications

References 113 publications

Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application

Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application

Oil-Contaminated Soil Remediation with Biodegradation by Autochthonous Microorganisms and Phytoremediation by Maize (Zea mays)

Green Technology as a Way of Cleaning the Environment from Petroleum Substances in South-Eastern Poland

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