Evaluation of the effect of incorporation of dibutyryl cyclic adenosine monophosphate in an in situ-forming hydrogel wound dressing based on oxidized alginate and gelatin

Balakrishnan, Biji; Mohanty, Mira; Fernandez, Adelaide C.; Mohanan, P.V.; Jayakrishnan, A.

doi:10.1016/j.biomaterials.2005.08.021

Cited by 114 publications

(66 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Such hydrogel system was chosen because Balakrishnan et al demonstrated that the hydrogel made from OA in 0.1 M borax + gelatin in water had good in vitro and in vivo biocompatibility and was suitable for cartilage regeneration, drug release, and wound dressing as an injectable adhesive biomimetic scaffold. 9,23,[29][30] To further improve its properties for new and wider clinical applications, we integrated electroactive degradable conducting polymer nanoparticle of nEOAs within this hydrogel. As presented in Table 1, adding nEOAs can easily adjust the gel time of hydrogels: the higher the weight content of nEOA in eGels, the longer the gel time.…”

Section: Synthesis Of Egelmentioning

confidence: 99%

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Wang¹,

Wang²,

Teng³

2016

IJN

View full text Add to dashboard Cite

Injectable electroactive hydrogels (eGels) are promising in regenerative medicine and drug delivery, however, it is still a challenge to obtain such hydrogels simultaneously possessing other properties including uniform structure, degradability, robustness, and biocompatibility. An emerging strategy to endow hydrogels with desirable properties is to incorporate functional nanoparticles in their network. Herein, we report the synthesis and characterization of an injectable hydrogel based on oxidized alginate (OA) crosslinking gelatin reinforced by electroactive tetraaniline-graft-OA nanoparticles (nEOAs), where nEOAs are expected to impart electroactivity besides reinforcement without significantly degrading the other properties of hydrogels. Assays of transmission electron microscopy, 1 H nuclear magnetic resonance, and dynamic light scattering reveal that EOA can spontaneously and quickly self-assemble into robust nanoparticles in water, and this nanoparticle structure can be kept at pH 3~9. Measurement of the gel time by rheometer and the stir bar method confirms the formation of the eGels, and their gel time is dependent on the weight content of nEOAs. As expected, adding nEOAs to hydrogels does not cause the phase separation (scanning electron microscopy observation), but it improves mechanical strength up to ~8 kPa and conductivity up to ~10 −6 S/cm in our studied range. Incubating eGels in phosphate-buffered saline leads to their further swelling with an increase of water content <6% and gradual degradation. When growing mesenchymal stem cells on eGels with nEOA content ≤14%, the growth curves and morphology of cells were found to be similar to that on tissue culture plastic; when implanting these eGels on a chick chorioallantoic membrane for 1 week, mild inflammation response appeared without any other structural changes, indicating their good in vitro and in vivo biocompatibility. With injectability, uniformity, degradability, electroactivity, relative robustness, and biocompatibility, these eGels may have a huge potential as scaffolds for tissue regeneration and matrix for stimuli responsive drug release.

show abstract

Section: Synthesis Of Egelmentioning

confidence: 99%

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Wang¹,

Wang²,

Teng³

2016

IJN

View full text Add to dashboard Cite

show abstract

“…The dressings were removed on days 2, 4, 8 and 12 post-surgery, and the wounds were examined and photographed to measure wound size reduction. The wound size measurements taken during surgery and biopsy were used to calculate the percentage size reduction using equation [30] Wound size reduction…”

Section: In Vivo Wound Healingmentioning

confidence: 99%

Development of fibroblast culture in three-dimensional activated carbon fiber-based scaffold for wound healing

Huang

Yeh

Lin³

et al. 2012

J Mater Sci: Mater Med

View full text Add to dashboard Cite

This work developed a novel bi-layer wound dressing composed of 3D activated carbon fibers that allows facilitates fibroblast cell growth and migration to a wound site for tissue reconstruction, and the gentamicin is incorporated into a poly(γ-glutamic acid)/gelatin membrane to prevent bacterial infection. In an in vitro, field emission scanning electron microscopy shows that rat skin fibroblasts appeared and spread on the surface of activated carbon fibers, and penetrated the interior and exterior of the 3D activated carbon fiber construct to a depth of roughly 200 μm. An in vivo analysis shows that fibroblast cells containing the proposed 3D scaffold had the potential of a biologically functionalized dressing to accelerate wound closure. Additionally, fibroblasts migrated to the wound site in a bi-layer wound dressing containing fibroblasts, enhancing fibronectin and type I collagen expression, resulting in faster skin regeneration than that achieved with a Tegaderm™ hydrocolloid dressing or gauze.

show abstract

“…In vivo experiment showed the wound covered with hydrogel was completely filled with new epithelium after two weeks using a rat model. In addition, incorporation of dibutyryl cyclic adenosine monophosphate (DBcAMP) into the in-situ forming hydrogels and sustained release of DBcAMP led to the enhancement in the rate of healing as well as reepithelialization of the wounds (Balakrishnan et al, 2006). Complete healing was achieved within 10 days associated with mild contracture of some of the wounds.…”

Section: Alginate-gelatin Hydrogelsmentioning

confidence: 99%

In-Situ Forming Biomimetic Hydrogels for Tissue Regeneration

Jin¹

2012

Biomedicine

View full text Add to dashboard Cite

Evaluation of the effect of incorporation of dibutyryl cyclic adenosine monophosphate in an in situ-forming hydrogel wound dressing based on oxidized alginate and gelatin

Cited by 114 publications

References 19 publications

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Development of fibroblast culture in three-dimensional activated carbon fiber-based scaffold for wound healing

In-Situ Forming Biomimetic Hydrogels for Tissue Regeneration

Contact Info

Product

Resources

About