Evaluation of the Effect of Experimental Cow Endometritis on Bactericidal Capability of Phagocytizing Cells Isolated from the Blood and Uterine Lumen

Abstract

Summary Studies were undertaken to assess the bactericidal activity of phagocytes isolated from blood and the uterine lumen of clinically healthy cows after ovulation, and from cows in which endometritis was induced experimentally. Experiments were carried out on 28 clinically healthy cows of the black and white lowland breed. Animals were aged 5 years and were used between the 2nd and 8th day after spontaneous ovulation. Cows were divided into four groups. Group I comprised animals in which cell‐mediated type immune reaction was induced in the left uterine horn by intrauterine challenge with tuberculin. Cows in this group were initially vaccinated with M. bovis via the intrauterine route. In group II, Arthus type immune reaction was induced by challenging immunized animals with C. fetus ssp. veneralis through intrauterine instillation. The non‐specific inflammatory process was initiated in the uterus of animals in group III by one instillation of lipopolysaccharide from S. abortus equi. Animals in group IV were set as control and received a phosphate buffered saline instillation into the uterine lumen. The cells from the left uterine horn were washed out 6 h after induction. Neutrophils were isolated from blood samples collected from all animals within the same time. The bacterial activity of cells from the uterine lumen and blood was assessed with the nitro‐blue tetrazolium reduction test. Results are presented as increase in optical density resulting from a constant number of phagocytizing cells (ΔOD/106 cells). Induction of cell‐mediated immune reaction or Arthus type immune reaction in the uterus significantly boosts the intracellular capability of uterine cells to kill bacteria through the oxidation system. Experimentally induced non‐specific endometritis weakens the bactericidal activity of uterine phagocytes, while peripheral blood phagocytes efficiently kill the engulfed bacteria.

Use of a Mycobacterial Cell Wall Extract (MCWE) in Susceptible Mares to Clear Experimentally Induced Endometritis With Streptococcus zooepidemicus

“…13 However, intrauterine administration of Mycobacterial sp. and tuberculin elicits PMN activation through cell-mediated immune reaction 36 but also may directly activate PMNs, as has been demonstrated with inactivated Streptococcus cells, 37 gram-negative cell wall components such as LPS, 38 the 19-kd lipoprotein from mycobacterium. 39…”

Effect of Baypamun® on Blood Leucocytes in Normal and Dexamathasone Treated Goats

“…The phagocytic activity of granulocytes was assessed by their ability to engulf standard bacterial strains, Staphylococcus aureus 305 and Smith, and bactericidal activity was assessed by spontaneous and stimulated reduction of nitro‐blue tetrazolium (NBT). Granulocytes were isolated and bacteria prepared according to standard procedures ( Kluciski et al . 1988 ) .…”

“…The numbers of phagocytizing cells and phagocytized bacteria were determined for further comparison. The NBT test was performed as described earlier ( Kluciski et al . 1988 ) .…”

Interactions of Metabolism, Inflammation, and Reproductive Tract Health in the Postpartum Period in Dairy Cattle

“…There are conflicting data about whether uterine PMN have more or less phagocytic activity than peripheral PMN (Klucinski et al. 1995).…”