Evaluation of the discriminatory power of variable number tandem repeat (VNTR) typing of Mycobacterium bovis strains

Hilty, Markus; Diguimbaye, Colette; Schelling, Esther; Baggi, Franca; Tanner, Marcel; Zinsstag, Jakob

doi:10.1016/j.vetmic.2005.05.017

Cited by 61 publications

(64 citation statements)

References 18 publications

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“…However, data from the previously published cluster in the West Midlands suggest that the 15-locus VNTR typing used in this study provides a high degree of discrimination (8). Others have suggested alternatives (1,12,22,25). Nevertheless, the small number of patients (five) among those born in the United Kingdom, where consumption of nonpasteurized milk was unlikely, who were not part of the group infected by the West Midlands outbreak strain suggests that transmission from pets is not a major public health issue.…”

Section: Discussionmentioning

confidence: 64%

“…Mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing can be used to successfully distinguish between M. bovis isolates, and when appropriate loci are selected, this approach may have a discriminatory power comparable to that of other genotyping methods (1,12,22,25). This method can therefore be used to confirm conventional epidemiological links, trace transmission routes, and identify sources of infection.…”

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confidence: 99%

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Investigating Transmission of Mycobacterium bovis in the United Kingdom in 2005 to 2008

Mandal¹,

Bradshaw²,

Anderson³

et al. 2011

J Clin Microbiol

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Due to an increase in bovine tuberculosis in cattle in the United Kingdom, we investigated the characteristics of Mycobacterium bovis infection in humans and assessed whether extensive transmission of M. bovis between humans has occurred. A cross-sectional study linking demographic, clinical, and DNA fingerprinting (using 15-locus mycobacterial interspersed repetitive-unit-variable-number tandem-repeat [MIRU-VNTR] typing) data on cases reported between 2005 and 2008 was undertaken. A total of 129 cases of M. bovis infection in humans were reported over the period, with a decrease in annual incidence from 0.065 to 0.047 cases per 100,000 persons. Most patients were born pre-1960, before widespread pasteurization was introduced (73%), were of white ethnicity (83%), and were born in the United Kingdom (76%). A total of 102 patients (79%) had MIRU-VNTR typing data. A total of 31 of 69 complete MIRU-VNTR profiles formed eight distinct clusters. The overall clustering proportion determined using the n ؊ 1 method was 33%. The largest cluster, comprising 12 cases, was indistinguishable from a previously reported West Midlands outbreak strain cluster and included those cases. This cluster was heterogeneous, having characteristics supporting recent zoonotic and humanto-human transmission as well as reactivation of latent disease. Seven other, smaller clusters identified had demographics supporting recrudescence rather than recent infection. A total of 33 patients had incomplete MIRU-VNTR profiles, of which 11 may have yielded 2 to 6 further small clusters if typed to completion. The incidence of M. bovis in humans in the United Kingdom remains low, and the epidemiology is predominantly that of reactivated disease.

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Section: Discussionmentioning

confidence: 64%

mentioning

confidence: 99%

Investigating Transmission of Mycobacterium bovis in the United Kingdom in 2005 to 2008

Mandal¹,

Bradshaw²,

Anderson³

et al. 2011

J Clin Microbiol

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“…The three loci which were not polymorphic in the M. bovis strains were MIRU10, Mtub12 and Mtub38. In previous studies on M. bovis strains isolated in Northern Ireland (Roring et al, 2004) and Chad (Hilty et al, 2005) MIRU10 had also been described as monomorphic. On the other hand, it was illustrated to be highly polymorphic with M. tuberculosis isolates in this study as well as in other M. tuberculosis strains isolated from South Africa, France and the United States (Hilty et al, 2005).…”

Section: Discussionmentioning

confidence: 95%

Molecular epidemiology of human and animal tuberculosis in Ibadan, Southwestern Nigeria

Jenkins¹,

Cadmus²,

Venter³

et al. 2011

Veterinary Microbiology

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From 2005 to 2007, Mycobacterium tuberculosis complex (MTC) strains were isolated from cattle, goats and pigs samples collected at the Bodija abattoir and from human samples from tuberculosis patients and livestock traders at the Akinyele cattle market in Ibadan, Southwestern Nigeria. Seventy four isolates obtained from humans (24) and livestock (50) were identified as MTC strains. Thirty two isolates were spoligotyped. Nineteen of these 32 isolates were identified as M. tuberculosis whilst 13 were identified as Mycobacterium bovis. M. bovis was isolated from two humans, whereas M. tuberculosis was isolated from a bovine, a pig and a goat. All the M. bovis isolates identified in this study belonged to the Africa 1 clonal complex. Multiple locus VNTR [variable number of tandem repeats] analysis (MLVA) was carried out on the 74 isolates. Three major clusters were defined. Group A consisted of 24 M. tuberculosis isolates (MLVA genotypes 1-18). One strain was isolated from a bovine and one from a pig. Group B consisted of 49 M. bovis strains (MLVA genotypes 19-48), mainly of cattle origin but also included four goat, nine pig and two human isolates. Group C consisted of a single M. tuberculosis isolate (MLVA genotype 49) obtained from a goat. Spoligotyping and MLVA confirmed it as clustering with the East Africa Indian clade found in humans in Sudan and the Republic of Djibouti. The isolation of three M. tuberculosis strains from livestock raises the question of their epidemiological importance as a source of infection for humans.

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“…Implementation of modern molecular biology methods and observation of new bTB outbreaks among cattle are crucial for the elimination strategy [20]. The main task for counteracting tuberculosis is the ability to find the sources of infection (case detection).…”

Section: Discussionmentioning

confidence: 99%

Animal Tuberculosis as a Potential Danger to Men

Krajewska¹,

Weiner²,

Augustynowicz–Kopeć³

2017

hpc

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Evaluation of the discriminatory power of variable number tandem repeat (VNTR) typing of Mycobacterium bovis strains

Cited by 61 publications

References 18 publications

Investigating Transmission of Mycobacterium bovis in the United Kingdom in 2005 to 2008

Investigating Transmission of Mycobacterium bovis in the United Kingdom in 2005 to 2008

Molecular epidemiology of human and animal tuberculosis in Ibadan, Southwestern Nigeria

Animal Tuberculosis as a Potential Danger to Men

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