Evaluation of the diagnostic performance and the utility of Helicobacter pylori stool antigen lateral immunochromatography assay

Abdelmalek, Shaymaa; Wafy, Hamed; Nagy, Neven; Shokry, Karim; Abdelrahman, Hisham A.

doi:10.1016/j.heliyon.2022.e09189

Cited by 5 publications

(3 citation statements)

References 25 publications

(44 reference statements)

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“…Similarly, Krausse et alfound a higher performance in SAT in males [ 21 ]. On the contrary, Abdelmalek et alfound no difference in the diagnostic performance of SAT in Egypt [ 22 ]. These findings may reflect potential differences in exposure mechanisms by gender roles.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic performance of fecal Helicobacter pylori antigen test in Uganda

et al. 2022

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We evaluated the diagnostic performance of a qualitative stool antigen test (SAT) in individuals with dyspepsia in rural Uganda using the polymerase chain reaction-based 16S ribosomal RNA assay (16S rRNA) for nucleotide sequences for two common H. pylori-associated genes as the reference standard. We enrolled 150 adults with dyspepsia with no self-reported use of antibiotic and/or antiacid medication medications within a fortnight. We performed blinded SAT on fecal specimens and 16S rRNA tests on gastric specimens. Using nonlinear mixed models, SAT had a sensitivity of 85⋅1% (95%CI 76⋅54%, 93⋅6%), and specificity of 97⋅6% (95%CI 94⋅3, 100). Twelve individuals with dyspepsia need to be tested to correctly diagnose 10 with H. pylori infection using SAT. The SAT is a robust diagnostic test to improve the diagnosis of H. pylori infection in people with dyspepsia in resource-limited settings.

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Section: Discussionmentioning

confidence: 99%

Diagnostic performance of fecal Helicobacter pylori antigen test in Uganda

et al. 2022

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“…Stool samples (from animals and humans) were examined for the presence of H. pylori -specific antigen using H. pylori Ag Rapid Test-Cassette (OnSite®, CTK Biotech, San Diego, USA), a lateral-flow chromatographic immunoassay for the qualitative detection of H. pylori antigen in stool samples [ 49 , 50 ]. The procedures and reading of the results were performed according to the manufacturer’s protocols.…”

Section: Methodsmentioning

confidence: 99%

An investigative study on the zoonotic potential of Helicobacter pylori

et al. 2023

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Background Helicobacter pylori is one of the most common bacterial infections and is widespread globally. It causes a variety of gastrointestinal disorders, though a great proportion of infections are asymptomatic. A total of 143 fresh stool samples were collected from apparently healthy farm and pet animals (43 cattle, 50 buffaloes, 50 sheep, 50 dogs, and 50 cats), in addition to 768 human stool samples. The samples were examined using stool antigen and rapid antibody tests, and further confirmation of glmM “human antigen-positive samples and animal milk samples” was conducted by polymerase chain reaction (PCR). Results The prevalence rates of H. pylori infection in animals were 22.2% and 16% in antibody and stool antigen tests, respectively. The detection rates were 28%, 24%, 12%, 10%, and 4.7% in cats, dogs, buffaloes, sheep, and cattle, respectively. On the other hand, the prevalence rate of H. pylori infection in human stool samples was 74.8%, and a statistically significant association was observed between prevalence and several factors, such as sex, age, and locality. PCR was performed to detect the glmM gene of H. pylori, and this gene was found in 21 of 27 human antigen-positive samples and 5 of 13 animal milk samples. Conclusions H. pylori was detected in both human and animal samples. Furthermore, glmM was found in milk and human samples. Our findings suggest that pet and farm animals could transmit H. pylori infection to humans.

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“…The LFIA is widely adopted in the prevention of contagious diseases and plays a critical role in research into the identification of infectious sources [ 7 , 8 , 9 ]. This method treats the target as an antibody.…”

Section: Introductionmentioning

confidence: 99%