Evaluation of the developmental toxicity of the aqueous extract from Trigonella foenum-graecum (L.) in mice

Khalki, Loubna; M’hamed, Sâadia Ba; Bennis, Mohamed; Chait, Abderrahman; Sokar, Zahra

doi:10.1016/j.jep.2010.06.033

Cited by 54 publications

(51 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One plant in particular, Trigonella foenum-graecum, or fenugreek (in Arabic, hulabah), has been used in Ayurvedic and Chinese medicine for numerous indications. Animal and human studies have demonstrated the hypoglycaemic and antihyperlipidemic properties of oral fenugreek seed powder, which are due to the antidiabetic compounds present in the seeds, leaves, and different type of extracts [50][51][52].…”

Section: Fenugreek (Trigonella Foenum-graecum L)mentioning

confidence: 99%

4-Hydroxyisoleucine from Fenugreek (Trigonella foenum-graecum): Effects on Insulin Resistance Associated with Obesity

Avalos-Soriano

Cruz-Cordero²,

Rosado

et al. 2016

Molecules

View full text Add to dashboard Cite

Obesity and insulin resistance (IR) are interdependent multifactorial processes that cannot be understood separately. Obesity leads to systemic inflammation and increased levels of free fatty acids that provoke IR and lipotoxicity. At the same time, IR exacerbates adipose cell dysfunction, resulting in chronic inflammation and major lipotoxic effects on nonadipose tissues. 4-Hydroxyisoleucine (4-OHIle), a peculiar nonprotein amino acid isolated from fenugreek (Trigonella foenum-graecum) seeds, exhibits interesting effects on IR related to obesity. 4-OHIle increases glucose-induced insulin release, and the insulin response mediated by 4-OHIle depends on glucose concentration. The beneficial effects observed are related to the regulation of blood glucose, plasma triglycerides, total cholesterol, free fatty acid levels, and the improvement of liver function. The mechanism of action is related to increased Akt phosphorylation and reduced activation of Jun N-terminal kinase (JNK)1/2, extracellular signal-regulated kinase (ERK)1/2, p38 mitogen-activated protein kinase (MAPK), and nuclear factor (NF)-κB. Here, we present a review of the research regarding the insulinotropic and insulin-sensitising activity of 4-OHIle in in vitro and in vivo models.

show abstract

Section: Fenugreek (Trigonella Foenum-graecum L)mentioning

confidence: 99%

4-Hydroxyisoleucine from Fenugreek (Trigonella foenum-graecum): Effects on Insulin Resistance Associated with Obesity

Avalos-Soriano

Cruz-Cordero²,

Rosado

et al. 2016

Molecules

View full text Add to dashboard Cite

show abstract

“…Moreover, hydroalcoholic extract of fenugreek seed at 50, 100 and 200 mg/kg by intraperitoneal injection for 20 days was also shown to reduce folliculogenesis and destroy ovary tissue in female Balb/C mice (Modaresi et al, 2012). It is also shown that fenugreek seed extract administered orally, produced a significant dose-dependent reduction in the number of fetuses per litter (Khalki et al, 2010).…”

Section: Discussionmentioning

confidence: 94%

“…However, published literature regarding the reproductive safety profile of fenugreek extracts showed inconsistently. These differences can be attributed mainly due to differences in standardization methods, marker compounds, use of solvents for extraction, compliance to applicable guidelines and route of administration used in these published studies (Ahirwar et al, 2010;Dande and Patil, 2012;Sharma and Bhinda, 2005;Araee et al, 2009;Aswar et al, 2009;Deshpande et al, 2016;Khalki et al, 2010;Mital and Gopaldas, 1976;Modaresi et al, 2012). For example, oral administration during day 1-10 of pregnancy of ethanol extract of fenugreek (500 mg/kg/day) seeds consisting mainly of alkaloids, saponins, and flavonoids, was found to have anti-implantation and estrogenic agent in female rat and mouse respectively (Ahirwar et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Prenatal developmental toxicity evaluation of IDM01, a botanical composition of 4-hydroxyisoleucine and trigonelline based standardized fenugreek seed extract, during organogenesis period of pregnancy in rats

2017

J App Pharm Sci

View full text Add to dashboard Cite

The present study was undertaken to assess the developmental toxicity of botanical composition of standardized fenugreek seed extract with markers 4-hydroxyisoleucine and trigonelline (IDM01) during organogenesis period of pregnancy in rats in accordance with OECD guideline (No. 414). Pregnant rats were administered by gavage at 125, 250, and 500 mg/kg per day of IDM01 over the exposure period of gestational days 5-19 (GD5-GD19). The vehicle control (VC) group was also maintained. The cesarean sections of dams on GD20 were done. All fetuses were examined for external, visceral, and skeletal alterations. IDM01 treatment did not adversely affect maternal parameters (body weights, uterine weight, uterine observations, feed consumption, and gross pathology), and fetal developmental parameters (fetal body weight, crown-rump length, sex ratio and incidence of anomalies) as compared to VC group. In conclusion, the prenatal oral exposure of IDM01 during organogenesis period to pregnant female rats is without any of maternal or developmental (fetotoxicity or teratogenicity) with "No Observed Adverse Effect Level" (NOAEL) greater than 500 mg/kg/day.

show abstract

“…: PIIUM 0226-2. Using a modified method of traditional medicinal practitioners, FSA extract was prepared 6 . The prepared fenugreek seed powder was put in distilled water in a ratio of 1 gram of powder in 20 ml of distilled water, followed by stirring for 24 hours using magnetic stirrer at room temperature.…”

Section: Preparation Of Fenugreek Seeds Aqueous Extract (Fsa)mentioning

confidence: 99%

Acute Toxicity of Trigonella Foenum-Graecum (Fenugreek) Seeds Aqueous Extract on Liver in Male Mice, Histopathological Study

Alfarisi¹,

Allow²,

Hamdan³

et al. 2017

Int. Res. J. Pharm.

View full text Add to dashboard Cite

Acute toxic effect of fenugreek on liver histology has not been fully explored. Hence the objective of this study is to investigate the acute toxicity of fenugreek seeds aqueous extract (FSA) on liver histology. Twelve male Swiss albino mice, were randomly divided into control (C), and three treatment (T1, T2, and T3) groups (n = 3 each). T1, T2, and T3 were given 3g, 6g, and 9g/kg body weight FSA respectively. Intragastric divided doses of FSA were given as per OECD guidelines 425. Continuous observation of signs of acute toxicity and survival set up. The mice were euthanized on day 14 and the liver was dissected and processed for histopathological examination. 3, 6, and 9g/kg body weight FSA doses failed to induce any of signs of acute toxicity. Histopathological examination revealed that, all FSA administered doses showed mild portal inflammation, mild mononuclear cell infiltration in hepatic parenchyma, in addition to mild bile stasis induced only in mice received 9g/kg of FSA. Administered doses of FSA showed mild liver histopathological inflammatory changes.

show abstract

Evaluation of the developmental toxicity of the aqueous extract from Trigonella foenum-graecum (L.) in mice

Cited by 54 publications

References 16 publications

4-Hydroxyisoleucine from Fenugreek (Trigonella foenum-graecum): Effects on Insulin Resistance Associated with Obesity

4-Hydroxyisoleucine from Fenugreek (Trigonella foenum-graecum): Effects on Insulin Resistance Associated with Obesity

Prenatal developmental toxicity evaluation of IDM01, a botanical composition of 4-hydroxyisoleucine and trigonelline based standardized fenugreek seed extract, during organogenesis period of pregnancy in rats

Acute Toxicity of Trigonella Foenum-Graecum (Fenugreek) Seeds Aqueous Extract on Liver in Male Mice, Histopathological Study

Contact Info

Product

Resources

About