Evaluation of the cytotoxicity and phototoxicity of Caryocar brasiliense supercritical carbon dioxide extract

Anjos

2020

RSD

O Caryocar brasiliense Camb. (pequizeiro) é uma árvore típica brasileira com alta concentração de compostos fenólico, de amplo uso popular para fins nutricionais e medicinais. Nessa perspectiva a presente pesquisa teve como objetivo realizar uma prospecção tecnológica avaliando o panorâma mundial de patentes depositadas. Foi utilizado o software de busca e análise Questel - Orbit, sendo incluídos os documentos que tinham “pequi”, “Caryocar brasiliense” ou “pequizeiro” no título, resumo ou reivindicações. Foram identificados 128 documentos de patentes. No ano de 2002 houve os primeiros depósitos, desde então todos os anos houve ao menos um depósito, até o momento que a pesquisa foi realizada. O principal país depositário foi o Brasil, os documentos identificados referem-se em diferentes processos aplicados para a obtenção de produtos para conversão química, com aplicação principalmente na área gastronômica e/ou farmacêutica. Dessa forma, a pesquisa aponta interesse, principalmente brasileiro, no desenvolvimento de tecnologias atribuídas a esta espécie.

Section: Introductionunclassified

Prospecção tecnológica do pequizeiro (Caryocar brasiliense camb.)

Anjos

2020

RSD

Anal. Method Environ. Chem. J.

“…Chlorpromazine (PIF=35.59) was a potential phototoxic hazard and results were obtained for the cell viability with a difference approximately 35-fold in EC 50 values, with and without UV light (Table 4). Amaral et al [39] presented that the IC 50 values for Caryocar brasiliense supercritical CO 2 extract (CBSE) in the phototoxicity assay considered 6.50% w/v in dark conditions and 35.53% w/v in irradiated conditions. According to the PIF value, the CBSE not exhibited phototoxic potential (PIF=0.18).…”

Section: Phototoxicity Analysismentioning

confidence: 99%

In-vitro evaluation of photoprotection, cytotoxicity and phototoxicity of aqueous extracts of Cuscuta campestris and Rosa damascene by MTT method and UV spectroscopy analysis

Khazaeli

Ameri

Mehrabani

et al. 2022

Applying sunscreen is essential for protecting the skin from UV’s acute and chronic effects. Some of these products on the market display side effects and are expensive. There is a great demand for effective, cheap, safe, and herbal sunscreens with a wide range of sun protection activities. This study aimed to evaluate the photoprotection, cytotoxicity, and phototoxicity of aqueous extracts of Cuscuta campestris (CC-AE) and Rosa damascena (RD-AE). The maceration method prepared the CC-AE and RD-AE from the aerial branch. In-vitro photoprotection was evaluated by determining the sun protective factor (SPF) of CC-AE and RD-AE by a UV-visible spectrophotometer. The cytotoxicity and phototoxicity studies were assessed using the MTT assay on 3T3 cells. In the final, the PIF (Photo Inhibitor Factor) was calculated. The SPF values of CC-AE and RD-AE were found at 11.10±0.05 and 1.36±0.04, respectively, at the concentration of 0.2 mg mL-1. The half maximal effective concentration (EC50) of CC-AE and RD-AE was obtained at 35.05±0.91 µg mL-1 and 40.7±0.87 µg mL-1, respectively. The phototoxicity analysis showed that CC-AE and RD-AE had low PIF values and were considered probable phototoxic. Overall, regarding SPF and PIFs values, the anti-inflammatory and antioxidant properties, can be evaluated for further pharmaceutical formulations.

“…The economic and cultural importance of the pequi fruit for the Cerrado population is well known, especially for those who work in family farming [ 4 ]. The pequi tree is the subject of industrial agriculture in Brazil, and the fruit of the pequi is extensively used in food and oil production; therefore, the raw materials are widely available [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Effect and Toxicological Profile of Pulp Residue from the Caryocar Brasiliense, a Sustainable Raw Material

Fracasso

Ibe

Costa

et al. 2023

Gels

Caryocar brasiliense Cambess is a plant species typical of the Cerrado, a Brazilian biome. The fruit of this species is popularly known as pequi, and its oil is used in traditional medicine. However, an important factor hindering the use of pequi oil is its low yield when extracted from the pulp of this fruit. Therefore, in this study, with aim of developing a new herbal medicine, we an-alyzed the toxicity and anti-inflammatory activity of an extract of pequi pulp residue (EPPR), fol-lowing the mechanical extraction of the oil from its pulp. For this purpose, EPPR was prepared and encapsulated in chitosan. The nanoparticles were analyzed, and the cytotoxicity of the encapsu-lated EPPR was evaluated in vitro. After confirming the cytotoxicity of the encapsulated EPPR, the following evaluations were performed with non-encapsulated EPPR: in vitro anti-inflammatory activity, quantification of cytokines, and acute toxicity in vivo. Once the anti-inflammatory activity and absence of toxicity of EPPR were verified, a gel formulation of EPPR was developed for topical use and analyzed for its in vivo anti-inflammatory potential, ocular toxicity, and previous stability assessment. EPPR and the gel containing EPPR showed effective anti-inflammatory activity and lack of toxicity. The formulation was stable. Thus, a new herbal medicine with anti-inflammatory activity can be developed from discarded pequi residue.