Evaluation of the cytotoxic and genotoxic effects by melamine and cyanuric acid co-exposure in human embryonic kidney 293 cells

Xu, Xianrong; Lu, Jeff Zhiqiang; Sheng, Hongqiang; Zhang, Long; Gan, Tieer; Zhang, Jianyun; Xu, Yuehua; Zhu, Xinqiang; Yang, Jun

doi:10.1590/1414-431x20209331

Cited by 7 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is noteworthy that melamine levels were signi cantly correlated with Per activity with or without added DNA. Melamine has 2 oxidation sites leading to ammelide and cyanuric acid (Shallileh et al, 2023), where melamine-cyanuric acid complex can lead to DNA damage and renal damage (Xu et al, 2020). Interestingly, single exposures of either cyanuric acid or melamine in human embryonic kidney 293 cells did not lead to DNA strand breaks but co-exposure to them produced DNA strand breaks.…”

Section: Discussionmentioning

confidence: 99%

Screening of municipal effluents with the peroxidase toxicity assay

Gagné,

André,

Smyth

2024

Preprint

View full text Add to dashboard Cite

The peroxidase (Per) reaction is a quick and inexpensive biosensor for the screening of environmental contaminants. The purpose of this study was to screen various municipal wastewaters before and after 7 different types of treatment processes. Wastewater samples before (influents) and after the following treatments (effluent) were tested using the Per activity test: advanced biofiltration, biofiltration, aerated lagoons, secondary aeration sludge, trickling filter, secondary membrane filtration, and primary. The influents and effluents were collected for 3 days composites and concentrated to 500 X on a reverse-phase (C18) extraction cartridge. The ethanol extracts were examined for dissolved organic carbon, plastic-like materials, polyaromatic hydrocarbons and polystyrene nanoplastics. The samples were then tested using the Per reaction alone and in the presence of DNA to detect DNA binding agents. The result show that population size tended to increase Per activity and 60% of the effluents decreased Per activity leading to H2O2 persistence. More advanced treatments (biofiltration, membrane biofiltration, secondary aeration) produced stronger changes from the corresponding untreated influents. The addition of DNA during the Per reaction revealed that population size had no influence and that significant changes occurred in 60% of treated effluents suggesting release of genotoxic compounds in the aquatic environment by most treated wastewaters. The toxic implications of these results to aquatic organisms are discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Screening of municipal effluents with the peroxidase toxicity assay

Gagné,

André,

Smyth

2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The co-exposure of cyanuric acid and melamine in animals and humans exhibited increased toxicity compared to individual exposure. Moreover, Xu et al ( 22 ) underscored the complex toxic effects arising from co-exposure to melamine and cyanuric acid, showing cytotoxic and genotoxic effects in human kidney cells, which in turn suggested the formation of melamine-cyanuric acid complex.…”

Section: Study On Health Risk and Control Standard Of Cyanuric Acid I...mentioning

confidence: 99%

Study on the health risk of cyanuric acid in swimming pool water and its prevention and control measures

Chen,

Su,

Chen

et al. 2024

Front. Public Health

View full text Add to dashboard Cite

Cyanuric acid is a widely used fine chemical intermediate that acts as a free chlorine buffer in swimming pool water, wherein it is often used as a stabilizer to maintain the germicidal efficacy of chlorinated disinfectants. However, it has also been associated with health risks. Herein, we introduced the sources and functions of cyanuric acid in swimming pool water, focusing on potential health risks associated with excessive concentration of the component and the current control standards worldwide. Also, the prevention and control measures were summarized in terms of physical chemistry, biodegradation, and ultraviolet radiation to provide a basis for the development of public health policies for swimming pool management.

show abstract

“…Among identifications, there were also large scale produced chemicals and/or polymer degradation products, such as melamine, triphenylphosphate, diphenylphtalate, insect repellent diethyltoluamide, and also the common drug ketoprofen. It was demonstrated that several of these chemicals could induce cytotoxicity in numerous cell lines: diclofenac in breast (MCF-7), cervical (HeLa) and colorectal (HT-29) cancers [77,78]; ketoprofen in the human embryonic kidney (HEK 293) [79]; DDAC-C10 in lung cancer (A-549) [80]; melamine in HEK 293, fibroblasts (L929) and Chinese hamster ovary (CHO) [81,82]; carbaryl in human umbilical vein endothelial cells (HUVEC) and rat adrenal pheochromocytoma (PC-12) [83,84]. In our studies, leachate no.…”

Section: Targeted and Untargeted Uplc-hrms Analysismentioning

confidence: 99%

Assessment of Physicochemical, Microbiological and Toxicological Hazards at an Illegal Landfill in Central Poland

Szulc

Okrasa

Nowak

et al. 2022

IJERPH

View full text Add to dashboard Cite

This study aimed to assess the physicochemical, microbiological and toxicological hazards at an illegal landfill in central Poland. The research included the analysis of airborne dust (laser photometer), the number of microorganisms in the air, soil and leachate (culture method) and the microbial diversity in the landfill environment (high-throughput sequencing on the Illumina Miseq); the cytotoxicity (PrestoBlue) and genotoxicity (alkaline comet assay) of soil and leachate were tested. Moreover, an analysis of UHPLC-Q-ToF-UHRMS (ultra-high-performance liquid chromatography-quadrupole-time-of-flight ultrahigh-resolution mass spectrometry) was performed to determine the toxic compounds and microbial metabolites. The PM1 dust fraction constituted 99.89% and 99.99% of total dust and exceeded the threshold of 0.025 mg m−3 at the tested locations. In the air, the total number of bacteria was 9.33 × 101–1.11 × 103 CFU m−3, while fungi ranged from 1.17 × 102 to 4.73 × 102 CFU m−3. Psychrophilic bacteria were detected in the largest number in leachates (3.3 × 104 to 2.69 × 106 CFU mL−1) and in soil samples (8.53 × 105 to 1.28 × 106 CFU g−1). Bacteria belonging to Proteobacteria (42–64.7%), Bacteroidetes (4.2–23.7%), Actinobacteria (3.4–19.8%) and Firmicutes (0.7–6.3%) dominated. In the case of fungi, Basidiomycota (23.3–27.7%), Ascomycota (5.6–46.3%) and Mortierellomycota (3.1%) have the highest abundance. Bacteria (Bacillus, Clostridium, Cellulosimicrobium, Escherichia, Pseudomonas) and fungi (Microascus, Chrysosporium, Candida, Malassezia, Aspergillus, Alternaria, Fusarium, Stachybotrys, Cladosporium, Didymella) that are potentially hazardous to human health were detected in samples collected from the landfill. Tested leachates and soils were characterised by varied cyto/genotoxins. Common pesticides (carbamazepine, prometryn, terbutryn, permethrin, carbanilide, pyrethrin, carbaryl and prallethrin), quaternary ammonium compounds (benzalkonium chlorides), chemicals and/or polymer degradation products (melamine, triphenylphosphate, diphenylphtalate, insect repellent diethyltoluamide, and drugs (ketoprofen)) were found in soil and leachate samples. It has been proven that the tested landfill is the source of the emission of particulate matter; microorganisms (including potential pathogens) and cyto/genotoxic compounds.

show abstract

Evaluation of the cytotoxic and genotoxic effects by melamine and cyanuric acid co-exposure in human embryonic kidney 293 cells

Cited by 7 publications

References 29 publications

Screening of municipal effluents with the peroxidase toxicity assay

Screening of municipal effluents with the peroxidase toxicity assay

Study on the health risk of cyanuric acid in swimming pool water and its prevention and control measures

Assessment of Physicochemical, Microbiological and Toxicological Hazards at an Illegal Landfill in Central Poland

Contact Info

Product

Resources

About