2022
DOI: 10.3390/ijerph19084826
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Assessment of Physicochemical, Microbiological and Toxicological Hazards at an Illegal Landfill in Central Poland

Abstract: This study aimed to assess the physicochemical, microbiological and toxicological hazards at an illegal landfill in central Poland. The research included the analysis of airborne dust (laser photometer), the number of microorganisms in the air, soil and leachate (culture method) and the microbial diversity in the landfill environment (high-throughput sequencing on the Illumina Miseq); the cytotoxicity (PrestoBlue) and genotoxicity (alkaline comet assay) of soil and leachate were tested. Moreover, an analysis o… Show more

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Cited by 12 publications
(6 citation statements)
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“…Recently there has been a rapid development of metagenomic methods that allow for the detection of the presence of entire genomes of microorganisms in a sample in various environments [ 28 , 29 ]. These are the methods derived from the systemic methods of molecular biology and allow for the sequence analysis of the obtained genetic material.…”
Section: Discussionmentioning
confidence: 99%
“…Recently there has been a rapid development of metagenomic methods that allow for the detection of the presence of entire genomes of microorganisms in a sample in various environments [ 28 , 29 ]. These are the methods derived from the systemic methods of molecular biology and allow for the sequence analysis of the obtained genetic material.…”
Section: Discussionmentioning
confidence: 99%
“…Air (8000 L) was passed through sterile gelatine filters (80 mm, 0.3 µL Sartorius, Göttingen, Germany) using AirPort MD 8 (Sartorius, Germany). The analysis was performed in accordance with the methodology described by Szulc et al, [ 29 ]. In brief, genomic DNA was extracted using Genomic Mini AX Bacteria + set (A&A Biotechnology, Gdańsk, Poland) with additional mechanical lysis with zirconium oxide beads of the samples (FastPrep-24).…”
Section: Methodsmentioning
confidence: 99%
“…Secondly, there are obvious differences between utilization levels, recycling technology, and recycling systems, indicating that industrial production technology has not been fully developed in the process of participating in recycling. Therefore, combined with the above analysis, China's solid waste resource recycling policy should strengthen integration with technology, promote the implementation of the policy with technology, and pay attention to the intersection and cooperation between different technologies, so as to truly benefit from the recycling of technology [30,31].…”
Section: Literature Research and Analysis Under The Dimension Of Tech...mentioning
confidence: 99%