Evaluation of the Chemical Composition and Antioxidant Activity of the Peel Oil ofCitrus nobilis

Abstract

Peel oil of Citrus nobilis (Lour) was analyzed for determining its chemical composition. Fourteen identified components accounted for 99.1% (GC) and 100.0% (FID) of the total oil. Major component of the oil was limonene (76.8%-GC and 86.2%-FID). Essential oil was also evaluated for its antioxidant activity in four complementary test systems namely; β-carotene/linoleic acid, DPPH radical scavenging, reducing power and metal chelating activities. In the first system, antioxidant activity increased with the increasing concentration. At 20.0 mg.ml −1 concentration, antioxidant property of the oil was 96.8% ± 0.2 and as strong as the positive controls BHT and α-tocopherol. Scavenging effect of the oil was superior to the positive controls BHT and α-tocopherol at 1.5 mg.ml −1 concentration (96.4% ± 0.1). Reducing power and chelating effect of the essential oil increased with the increasing concentration.

Antioxidant and Antihemolytic Activities of Ethanolic Extract of Flowers, Leaves, and Stems ofHyssopus officinalisL. Var.angustifolius

“…The stable 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical was used for determination of free radical-scavenging activity of the extracts. [9,10] Different concentrations of the sample were added, at an equal volume, to ethanolic solution of DPPH (100 µM). After 15 min at room temperature, the absorbance was recorded at 517 nm.…”

Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages

“…Almost all biomolecules, but mainly biomembranes, proteins and nucleic acids, may be attacked by reactive free radicals. Free radicals are responsible for many pathological processes, or they can be generated as the result of the pathological stage and cause important secondary damage to biological systems and cells [ 1 , 4 , 5 , 6 , 7 ]. Connections between free radicals and some serious diseases, including Parkinson´s and Alzheimer´s disease, atherosclerosis, heart attacks, and chronic fatigue syndrome, have been demonstrated.…”

“…Determination of the antioxidant activity is one of the ways how to biologically and nutritionally evaluate the quality of the fruit. It has been proved that antioxidant activity depends on the type of phenolics present in the fruit, as some phenolic compounds exhibit higher antioxidant activity than others [ 7 , 9 , 19 , 20 , 21 , 22 , 25 , 26 , 27 , 28 , 29 ]. It is assumed that the ability of plant polyphenols to scavenge reactive oxygen radicals participates in the protective mechanism of plants.…”

“…Due to the chemical diversity of antioxidants present in fruit, their strictly defined content is unavailable. In spite of the fact that total amount of antioxidants in various fruit types need not to represent the total antioxidant capacity [ 2 , 4 , 5 , 7 , 9 , 19 , 20 , 21 , 22 , 25 , 26 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ], almost all phenolic compounds in fruits demonstrate some antioxidant activity [ 1 , 2 , 3 , 4 , 7 , 9 , 10 , 11 , 12 , 13 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 29 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ]. However, detection of therapeutically active components in a biological matrix is a very complex procedure, and thei...…”

In Vitro Antioxidant and Antihemolytic Activities of Hydroalcoholic Extracts of Allium scabriscapum Boiss. & Ky. Aerial Parts and Bulbs

“…Superoxide indirectly initiates lipid peroxidation because superoxide anion acts as a precursor of singlet oxygen and hydroxyl radical. [24][25][26][27] Hydroxyl radicals eliminate hydrogen atoms from the membrane lipids, which results in lipid peroxidation. Antihemolytic activity of quercetin and other flavonoids have previously reported and good activity of extracts may be the result of high flavonoid content especially quercetin.…”