Introduction:Studies have suggested that free radicals-induced neurodegeneration is one of the many studies of Parkinson Disease (PD). Quercetin as a natural polyphenol has been regarded as a significant player in altering the progression of neurodegenerative diseases by protecting from damages caused by free radicals. Owing to its poor water solubility, preparation of its oral formulation is urgently needed. Recently, nanocrystal technique as an effective way has been introduced for oral administration of drugs.Methods:This study investigated the neuroprotective effects of quercetin nanocrystals on 6-hydroxydopamine (6-OHDA)-induced Parkinson-like model in male rats. Quercetin nanocrystals were prepared by the Evaporative Precipitation of Nanosuspension (EPN) method.Results:Administration of quercetin and its nanocrystals (10 and 25 mg/kg) prevented disruption of memory, increased antioxidant enzyme activities (superoxide dismutase and catalase) and total glutathione and reduced Malondialdehyde (MDA) level in the hippocampal area.Conclusion:The present study results demonstrated that quercetin nanocrystals with greater bioavailability is effective than quercetin alone in treatment of Parkinson-like model in rat.
In this study, antioxidant and antihemolytic activities of ethanolic extract of flowers, leaves, and stems of Hyssopus officinalis L. Var. angustifolius were investigated employing different in vitro assay systems. Extracts showed good antioxidant activity. IC 50 for 1,1-diphenyl-2picryl hydrazyl radical-scavenging activity were 148.8 ± 4.31 µg mL −1 for flowers, 79.9 ± 2.63 µg mL −1 for stems, and 208.2 ± 6.45 µg mL −1 for leaves. All extracts showed moderate iron (II) chelating ability. Extracts exhibited good antioxidant activity in the hemoglobininduced linoleic acid model and also they were capable of scavenging hydrogen peroxide in a concentration dependent manner. Extracts showed good antihemolytic activity againts hydrogen peroxide-induced hemolysis (IC 50 were 48.51 ± 2.27 µg mL −1 for flowers, 19.47 ± 0.73 µg mL −1 for leaves, and 63.1 ± 2.65 µg mL −1 for stems). The total amount of phenolic compounds in the extracts was determined as gallic acid equivalents and total flavonoid content was calculated as quercetin equivalents from a calibration curve.
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