Evaluation of the bioactivity of fluoride‐enriched mineral trioxide aggregate on osteoblasts

Proksch, Susanne; Brossart, J.; Vach, Kirstin; Hellwig, Elmar; Altenburger, Markus Jörg; Karygianni, Lamprini

doi:10.1111/iej.12905

Cited by 4 publications

(3 citation statements)

References 43 publications

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“…Biomineralization is the process by which a living organism synthesizes mineral substance. One important challenge facing endodontics today is precisely the correct formation of hard mineral tissue . To achieve this, it is essential that the materials used in endodontic treatments be bioactive.…”

Section: Discussionmentioning

confidence: 99%

Higher hydration performance and bioactive response of the new endodontic bioactive cement MTA HP repair compared with ProRoot MTA white and NeoMTA plus

2019

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The aim of this study was to characterize the hydration performance and the bioactive response of the new bioactive endodontic cement MTA HP repair (HP), comparing its physicochemical parameters with those of ProRoot MTA White (Pro) and NeoMTA Plus (Neo). Un-hydrated precursor materials were characterized by X-ray fluorescence, laser diffraction, N 2 physisorption and field emission gun scanning electron microscopy (FEG-SEM). Setting time was assessed according to ASTM specification C 266. Hydrated materials were analyzed by X-ray diffraction, Fourier transform infrared spectroscopy (FT-IR) and (FEG-SEM). Bioactivity evaluation in vitro was carried out, by soaking processed cement disk in simulated body fluid (SBF) during 168 h. The cements surface was studied by FT-IR, FEG-SEM, and energy dispersive X-ray. Release to the SBF media of ionic degradation products was monitored using inductively coupled plasma atomic emission spectroscopy. HP showed shorter initial setting time compared to Pro and Neo and produce a quick and effective bioactive response in vitro in terms of phosphate phase surface coating formation. This higher bioactive response for HP is correlated with increasing calcium aluminate content, increasing surface area of un-hydrated powder precursor and the increasing release capacity of Si ionic products of the final hydrated product. The higher bioactive response of MTA HP repair highlights this material, as very interesting to further investigate its performance to improve the outcome of vital pulp therapy procedures.

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Section: Discussionmentioning

confidence: 99%

Higher hydration performance and bioactive response of the new endodontic bioactive cement MTA HP repair compared with ProRoot MTA white and NeoMTA plus

2019

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“…Osteoblasts. Proksch et al (156) found that MTA impairs proliferation, osteogenic differentiation and extracellular matrix mineralization of primary human osteoblasts derived from the alveolar bone; this could be restored by addition of fluoride to MTA. Similarly, MTA inhibits proliferation and COL1 gene expression in bone marrow osteoblasts from rat femur (157).…”

Section: Osteoblasts/osteoclastsmentioning

confidence: 99%

In vitro biocompatibility and bioactivity of calcium silicate‑based bioceramics in endodontics (Review)

Song

Tang

et al. 2021

Int J Mol Med

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“…Moreover, bacterial biofilms may also cause persistent intraradicular and extraradicular infection leading to the destruction of periapical bone tissue (Chrepa et al, 2017). In the recent years, research are focused on promoting the regeneration of periapical bone tissue in refractory apical periodontitis therapies (Gjoksi et al, 2017; Proksch et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The bioceramic sealer iRoot SP promotes osteogenic differentiation of human stem cells from apical papilla via miR‐141‐3p/SPAG9/MAPK signalling pathway

Xue

et al. 2023

Int Endodontic J

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AimThe premixed bioceramic sealer iRoot SP that is widely used clinically has been reported to kill bacterial biofilms and promote osteogenic differentiation of human stem cells from the apical papilla (hSCAPs). Although miR‐141‐3p has been substantiated to be involved in the osteogenic process, the underlying mechanisms remain unclear. The aim of this study was to investigate the role of miR‐141‐3p in osteogenic differentiation and underlying mechanisms of iRoot SP‐treated hSCAPs.MethodologyhSCAPs were extracted from tissue blocks with enzyme digestion and identified by using immunofluorescence, flow cytometry and alizarin red staining. The mRNA expression level of miR‐141‐3p in hSCPAs after culture with iRoot SP was examined by quantitative real‐time PCR (qRT‐PCR) assay. SPAG9 was identified as a downstream target gene of miR‐141‐3p by dual‐luciferase report assay. Alkaline phosphatase (ALP) staining and activity detection, alizarin red staining, calcium concentration assay, qRT‐PCR and western blot were used to estimate osteogenic differentiation ability and involved protein expression levels of the osteogenic makers and signalling pathway‐related factors in iRoot SP‐treated hSCAPs. Data were analysed by one‐way anova and post hoc Tukey's test to determine any statistical differences between the experimental groups and the control group. p < .05 was considered statistically significant.ResultsExpression of miR‐141‐3p was reduced in iRoot SP‐treated hSCAPs with the increased exposure time up to 7 days, and the western blot and qRT‐PCR results revealed that the osteogenic markers osteocalcin (OCN), osterix (OSX), runt‐related transcription factor 2 (RUNX2) and dentin sialophosphoprotein (DSPP) were inversely correlated with miR‐141‐3p. The negative regulatory relationship between miR‐141‐3p and SPAG9/ mitogen‐activated protein kinases (MAPK) signalling axis was validated in this in vitro experiments.ConclusionsThe bioceramic sealer iRoot SP promoted osteogenic differentiation of hSCAPs by inhibiting miR‐141‐3p following down‐regulated SPAG9 expression, and activated MAPK pathway. These findings proposed a novel therapeutic impact of bioceramic sealer iRoot SP inducing bone regeneration in refractory periapical periodontitis.

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Evaluation of the bioactivity of fluoride‐enriched mineral trioxide aggregate on osteoblasts

Abstract: The addition of fluoride modulated the biocompatibility of MTA in terms of supporting bone cell proliferation and hard tissue formation. Hence, fluoride enrichment is a trend-setting advancement for MTA-based endodontic therapies.

Cited by 4 publications

References 43 publications

Higher hydration performance and bioactive response of the new endodontic bioactive cement MTA HP repair compared with ProRoot MTA white and NeoMTA plus

Higher hydration performance and bioactive response of the new endodontic bioactive cement MTA HP repair compared with ProRoot MTA white and NeoMTA plus

In vitro biocompatibility and bioactivity of calcium silicate‑based bioceramics in endodontics (Review)

The bioceramic sealer iRoot SP promotes osteogenic differentiation of human stem cells from apical papilla via miR‐141‐3p/SPAG9/MAPK signalling pathway

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