Evaluation of the BD Stem Cell Enumeration Kit on the BD FACSCanto II flow cytometer using bd facscanto clinical and bd facsdiva software

Abstract

Transplantation of hematopoietic stem cells (HSC) has been a widely used therapeutic option in patients with hematologic malignancies, congenital or acquired bone marrow failure, immunodeficiency states and autoimmunity for decades. 1 Peripheral blood has become the most commonly used source of HSC in both autologous and allogeneic transplantation due to easier accessibility and faster engraftment. Peripheral blood stem cells (PBSC) are collected with leukapheresis procedure, preceded by mobilization of HSC into the

Role of flow cytometry in evaluation of the cellular therapy products used in haematopoietic stem cell transplantation

“…Sample stability study is an important part of validation of the protocol used for quality assessment, especially when implementing a new type of cell therapy product. Several studies have examined the stability of fresh leukapheresis samples and thawed cryopreserved UCB samples 37,39,54,55 . The results showed that fresh leukapheresis samples were stable up to 24 h stored at 4°C 37,39 .…”

“…Several studies have examined the stability of fresh leukapheresis samples and thawed cryopreserved UCB samples 37,39,54,55 . The results showed that fresh leukapheresis samples were stable up to 24 h stored at 4°C 37,39 . The results of stability studies of thawed UCB samples were conflicting.…”

“…The advantage of these kits is that they contain pre‐assembled Ab panels, which facilitates the creation and implementation of protocols in laboratories that have no experience with setting up in‐house methods. Furthermore, some manufacturers offer software for acquisition and analysis which have an auto‐gating algorithm for isolation of the cells of interest, which also contributes to more user‐friendly cell analysis, for example, BD FACSCanto Clinical software (BD Bioscience, San Jose, CA) 39 …”

“…Furthermore, some manufacturers offer software for acquisition and analysis which have an auto-gating algorithm for isolation of the cells of interest, which also contributes to more user-friendly cell analysis, for example, BD FACSCanto Clinical software (BD Bioscience, San Jose, CA). 39 Transplantation of allogeneic HSC is sometimes followed with additional administration of donor lymphocytes, for example, donor lymphocyte infusion (DLI). Lymphocytes for DLI could be collected during allo-HSC collection procedure or from unstimulated PB, and cryopreserved as a simple and effective therapeutic option for patients in case of disease relapse.…”

“…Anti-CD34 Haematopoietic stem cells [14,17,18,23,24,33,34,37,39,43,45,47,48,[54][55][56] Anti-CD3 T cells [38,40,45,47,48,50,52] Anti-CD19 Anti-CD20…”