Evaluation of the Bactec Mgit 960 Tb System for Recovery and Identification of Mycobacterium Tuberculosis Complex in a High Through Put Tertiary Care Centre

Abstract

MGIT 960 system with PNBA assay for identification of M. tuberculosis complex is a rapid and useful method in laboratories processing a large number of specimens.

Evaluation of the BACTEC MGIT 960 TB with Solid Media for Recovery of Mycobacteria from Extrapulmonary Specimens in South Tunisia

“…Indeed, even though the LJ and the Coletsos medium were counted together as a single system, only 77 (78.5%) isolates of MTBC were recovered, in contrast to 92 (93.8%) isolates with the MGIT 960 system. These results are in accordance with those of other studies dealing mainly with pulmonary specimens [11][12][13][14][15].…”

“…Almost 19% of the samples were positive by LJ after eight weeks highlighting the importance of solid media incubation for a longer time (at least 10-12 weeks). For LJ medium, similar TTD were observed by Rodrigues et al which time positivity of MGIT960 was 91% during the first 2 weeks and only 4% for the LJ medium [15].…”

“…Rishi et al [16] and Rodrigues et al [15] found high rates (51.6% and 42% respectively). This can be explained by the fact that these authors had included both pulmonary and extrapulmonary tuberculosis samples.…”

Comparison of Mycobacterium tuberculosis complex Yield and Contamination Rates using Lowenstein-Jensen with and without Antibiotics in Western Kenya

“…While liquid culture techniques have been shown to have a higher yield and shorter time to detection of MTBC than solid media such as LJ, liquid culture methods are technically demanding and remain costprohibitive for routine use in many high-burden countries [35,36] . One challenge frequently encountered with routine use of liquid culture methods is the increase in overgrowth of bacterial contaminants compared to LJ [35] .…”

True facets of TB diagnosis in 2012: Hypes and realities

“…Scores of studies including at national TB control program level across the globe have compared the added advantages of automated liquid culture over solid culture, to cite a few a department health study conducted at Bangkok, Thailand (n = 2566) showed 53% positive on liquid culture vs 39% in solid culture with time to detection shortened to 11 days vs 27 days, respectively, with significant value addition [8]; another Indian study showed 41% culture positive in MGIT culture vs 24% in solid culture with time to detection of 9 days vs 38 days, respectively (n = 14,957). In the Indian study, MGIT showed its significant value addition in case of sputum smear negative samples with 18% positivity by MGIT vs 1% over solid culture with time to detection of 16 days vs 48 days, respectively [9]. There are also reported evidences from comparative evaluation for Mtb complex and NTM in liquid culture (MGIT 960) vs solid culture (LJ/Ogawa) clearly indicating substantial added value of liquid culture in terms of sensitivity as well as reduced time to detection [10].…”