Evaluation of the Aspergillus Western Blot IgG Kit for Diagnosis of Chronic Aspergillosis

Oliva, A; Flori, Pierre; Hennequin, Christophe; Dubus, J.‐C.; Reynaud‐Gaubert, Martine; Charpin, D.; Vergnon, Jean‐Michel; Colly, A; Piarroux, Renaud; Pelloux, Hervé; Ranque, Stéphane

doi:10.1128/jcm.02690-14

Cited by 35 publications

(33 citation statements)

References 25 publications

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“…34 A few studies have investigated the role of automated A. fumigatusspecific IgG assays in patients with ABPA complicating asthma. 30,[35][36][37][38] The results of these studies clearly suggest that the commercial to be a useful investigation in the classification of aspergillosis in adult cystic fibrosis. 39 The A. fumigatus-specific IgG is also the most useful investigation in the diagnosis of chronic pulmonary aspergillosis.…”

Section: Discussionmentioning

confidence: 80%

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Role of Aspergillus fumigatus‐specific IgG in diagnosis and monitoring treatment response in allergic bronchopulmonary aspergillosis

Agarwal

Dua

Choudhary

et al. 2016

Mycoses

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Few studies have evaluated the utility of Aspergillus fumigatus-specific IgG in allergic bronchopulmonary aspergillosis (ABPA). Herein, we evaluate the role of specific IgG in diagnosis and monitoring treatment response in ABPA. Forty-eight control subjects with A. fumigatus-associated asthma underwent A. fumigatus-specific IgG measurements at baseline, while specific IgG was assayed in 102 treatment-naïve subjects of ABPA at baseline, after eight weeks of glucocorticoid therapy, and during exacerbations. For determining the cut-off of A. fumigatus-specific IgG, we randomly classified two-thirds of the study subjects (cases and controls) as the derivation cohort, while the remaining one-thirds were labelled as the validation cohort. The best cut-off value of A. fumigatus-specific IgG in the derivation cohort was 26.9 mg /L (sensitivity: 88%; specificity: 100%). Using this limit, the sensitivity and specificity of A. fumigatus-specific IgG in diagnosis of ABPA was 89% and 100%, respectively, in the validation cohort. In contrast, the sensitivity of Aspergillus precipitins was only 27.4%. Following treatment, the A. fumigatus-specific IgG increased in 38 (37.2%) subjects, while it decreased in three (23.1%) of the 13 subjects experiencing an exacerbation. The A. fumigatus-specific IgG was found to be an extremely useful test in the diagnosis and differential diagnosis of ABPA but is unreliable in monitoring treatment response in this disorder.

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Section: Discussionmentioning

confidence: 80%

“…A few studies have investigated the role of automated A. fumigatus ‐specific IgG assays in patients with ABPA complicating asthma . The results of these studies clearly suggest that the commercial A. fumigatus ‐specific IgG assays perform better than Aspergillus precipitins in the detection of ABPA.…”

Section: Discussionmentioning

confidence: 99%

Role of Aspergillus fumigatus‐specific IgG in diagnosis and monitoring treatment response in allergic bronchopulmonary aspergillosis

Agarwal

Dua

Choudhary

et al. 2016

Mycoses

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“…Another drawback of this test is the need to dilute and retest all samples yielding values of Ͼ80 AU/ml. Furthermore, one recent study evaluated a commercial Western blot kit for the detection of anti-Aspergillus IgG (27). The authors reported a sensitivity of 90.0% to 93.8% in CPA and ABPA patients, with the specificity (94%) being estimated solely on the basis of the results for healthy blood donors as controls.…”

Section: Discussionmentioning

confidence: 99%

“…We decided to consider them controls, as a diagnosis of infection had been ruled out in these patients. Conversely, Aspergillus species colonization may be considered a prerequisite or initial stage of infection, accounting for the grouping together of colonized and infected patients in other studies (27). We also performed an analysis in which colonized individuals were grouped with the patients.…”

Section: Discussionmentioning

confidence: 99%

Prospective Evaluation of a New Aspergillus IgG Enzyme Immunoassay Kit for Diagnosis of Chronic and Allergic Pulmonary Aspergillosis

et al. 2016

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j Anti-Aspergillus IgG antibodies are important biomarkers for the diagnosis of chronic pulmonary aspergillosis (CPA) and allergic bronchopulmonary aspergillosis (ABPA). We compared the performance of a new commercial enzyme immunoassay (EIA) (Bordier Affinity Products) with that of the Bio-Rad and Virion‫گ‬Serion EIAs. This assay is novel in its association of two recombinant antigens with somatic and metabolic antigens of Aspergillus fumigatus. In a prospective multicenter study, 436 serum samples from 147 patients diagnosed with CPA (136 samples/104 patients) or ABPA (94 samples/43 patients) and from 205 controls (206 samples) were tested. We obtained sensitivities of 97%, 91.7%, and 86.1%, and specificities of 90.3%, 91.3%, and 81.5% for the Bordier, Bio-Rad, and Virion‫گ‬Serion tests, respectively. The Bordier kit was more sensitive than the Bio-Rad kit (P < 0.01), which was itself more sensitive than the Virion‫گ‬Serion kit (P ‫؍‬ 0.04). The Bordier and Bio-Rad kits had similar specificity (P ‫؍‬ 0.8), both higher than that of the Virion‫گ‬Serion kit (P ‫؍‬ 0.02). The area under the receiver operating characteristic (ROC) curves confirmed the superiority of the Bordier kit over the Bio-Rad and the Virion‫گ‬Serion kits (0.977, 0.951, and 0.897, respectively; P < 0.01 for each comparison). In a subset analysis of 279 serum samples tested with the Bordier and Bio-Rad kits and an in-house immunoprecipitin assay (IPD), the Bordier kit had the highest sensitivity (97.7%), but the IPD tended to be more specific (71.2 and 84.7%, respectively; P ‫؍‬ 0.10). The use of recombinant, somatic, and metabolic antigens in a single EIA improved the balance of sensitivity and specificity, resulting in an assay highly suitable for use in the diagnosis of chronic and allergic aspergillosis.

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“…Four protein bands at 16, 18 to 20, 22, and 30 kDa have been shown to be specific for Aspergillus sensitization. The first evaluation was published recently (9). Commercially available kits facilitate the standardization required to comply with accreditation according to ISO 15189.…”

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confidence: 99%

New Commercially Available IgG Kits and Time-Resolved Fluorometric IgE Assay for Diagnosis of Allergic Bronchopulmonary Aspergillosis in Patients with Cystic Fibrosis

Barrera

Richaud-Thiriez

Rocchi

et al. 2016

Clin Vaccine Immunol

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c Allergic bronchopulmonary aspergillosis (ABPA) is difficult to diagnose; diagnosis relies on clinical, radiological, pathological, and serological criteria. Our aim was to assess the performance of two new commercially available kits and a new in-house assay: an Aspergillus fumigatus enzyme-linked immunosorbent assay (ELISA) IgG kit (Bordier Affinity Products), an Aspergillus Western blotting IgG kit (LDBio Diagnostics), and a new in-house time-resolved fluorometric IgE assay (dissociation-enhanced lanthanide fluorescent immunoassay, or DELFIA) using recombinant proteins from an Aspergillus sp. recently developed by our laboratory for ABPA diagnosis in a retrospective study that included 26 cystic fibrosis patients. Aspergillus fumigatus-specific IgG levels measured by a commercial ELISA kit were in accordance with the level of precipitins currently used in our lab. The ELISA kit could accelerate and help standardize ABPA diagnosis. Aspergillus fumigatus-specific IgE levels measured by Immuno-CAP (Phadia) with A. fumigatus M3 antigen and by DELFIA with a purified protein extract of A. fumigatus were significantly correlated (P < 10 ؊6 ). The results with recombinant antigens glucose-6-phosphate isomerase and mannitol-1-phosphate dehydrogenase were encouraging but must be confirmed with sera from more patients. The DELFIA is an effective tool that can detect specific IgE against more fungal allergens than can be detected with other commercially available tests.

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Evaluation of the Aspergillus Western Blot IgG Kit for Diagnosis of Chronic Aspergillosis

Cited by 35 publications

References 25 publications

Role of Aspergillus fumigatus‐specific IgG in diagnosis and monitoring treatment response in allergic bronchopulmonary aspergillosis

Role of Aspergillus fumigatus‐specific IgG in diagnosis and monitoring treatment response in allergic bronchopulmonary aspergillosis

Prospective Evaluation of a New Aspergillus IgG Enzyme Immunoassay Kit for Diagnosis of Chronic and Allergic Pulmonary Aspergillosis

New Commercially Available IgG Kits and Time-Resolved Fluorometric IgE Assay for Diagnosis of Allergic Bronchopulmonary Aspergillosis in Patients with Cystic Fibrosis

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