Prospective Evaluation of a New Aspergillus IgG Enzyme Immunoassay Kit for Diagnosis of Chronic and Allergic Pulmonary Aspergillosis

Dumollard, C.; Bailly, Sébastien; Perriot, Sylvain; Brenier-Pinchart, Marie-Pierre; Saint-Raymond, Christel; Camara, B.; Gangneux, Jean‐Pierre; Persat, Florence; Valot, Stéphane; Grenouillet, Frédéric; Pelloux, Hervé; Pinel, Claudine; Cornet, Muriel

doi:10.1128/jcm.03261-15

Cited by 42 publications

(58 citation statements)

References 28 publications

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“…34 A few studies have investigated the role of automated A. fumigatusspecific IgG assays in patients with ABPA complicating asthma. 30,[35][36][37][38] The results of these studies clearly suggest that the commercial to be a useful investigation in the classification of aspergillosis in adult cystic fibrosis. 39 The A. fumigatus-specific IgG is also the most useful investigation in the diagnosis of chronic pulmonary aspergillosis.…”

Section: Discussionmentioning

confidence: 81%

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Role of Aspergillus fumigatus‐specific IgG in diagnosis and monitoring treatment response in allergic bronchopulmonary aspergillosis

Agarwal

Dua

Choudhary

et al. 2016

Mycoses

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Few studies have evaluated the utility of Aspergillus fumigatus-specific IgG in allergic bronchopulmonary aspergillosis (ABPA). Herein, we evaluate the role of specific IgG in diagnosis and monitoring treatment response in ABPA. Forty-eight control subjects with A. fumigatus-associated asthma underwent A. fumigatus-specific IgG measurements at baseline, while specific IgG was assayed in 102 treatment-naïve subjects of ABPA at baseline, after eight weeks of glucocorticoid therapy, and during exacerbations. For determining the cut-off of A. fumigatus-specific IgG, we randomly classified two-thirds of the study subjects (cases and controls) as the derivation cohort, while the remaining one-thirds were labelled as the validation cohort. The best cut-off value of A. fumigatus-specific IgG in the derivation cohort was 26.9 mg /L (sensitivity: 88%; specificity: 100%). Using this limit, the sensitivity and specificity of A. fumigatus-specific IgG in diagnosis of ABPA was 89% and 100%, respectively, in the validation cohort. In contrast, the sensitivity of Aspergillus precipitins was only 27.4%. Following treatment, the A. fumigatus-specific IgG increased in 38 (37.2%) subjects, while it decreased in three (23.1%) of the 13 subjects experiencing an exacerbation. The A. fumigatus-specific IgG was found to be an extremely useful test in the diagnosis and differential diagnosis of ABPA but is unreliable in monitoring treatment response in this disorder.

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Section: Discussionmentioning

confidence: 81%

“…A few studies have investigated the role of automated A. fumigatus ‐specific IgG assays in patients with ABPA complicating asthma . The results of these studies clearly suggest that the commercial A. fumigatus ‐specific IgG assays perform better than Aspergillus precipitins in the detection of ABPA.…”

Section: Discussionmentioning

confidence: 99%

Role of Aspergillus fumigatus‐specific IgG in diagnosis and monitoring treatment response in allergic bronchopulmonary aspergillosis

Agarwal

Dua

Choudhary

et al. 2016

Mycoses

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“…The characteristics of the included studies are presented in S1Table. The earliest study was published in 1983 [22] and the five most current articles were published in 2015 [23], 2016 [24, 25, 26] and 2018 [27]. Nine studies took place in five countries: Japan [25, 28], Brazil [23], United Kingdom [24, 29], France [26, 30, 31] and India [27], but in 4 articles, the study countries were not reported [22, 32, 33, 34].…”

Section: Resultsmentioning

confidence: 99%

“…Nine articles presented DID as the reference test [22, 23, 25, 27, 28, 30, 32, 33, 34]; an article presented two reference tests, DID and CIE [34] and four studies presented only CIE as the reference test [24, 26, 29, 31].…”

Section: Resultsmentioning

confidence: 99%

“…When we performed data extraction, some important differences were observed and deserve to be highlighted. Seven articles performed in-house ELISA tests [22, 23, 28, 30, 32, 33, 34] and six articles described their studies with commercial tests [24, 25, 26, 27, 29, 31]. Different Aspergillus antigens and cut-off points were used in the in-house ELISA tests; the articles that used commercial tests also used several types of antigens and cutoff points included by authors beyond those established by the manufacturer and are described in S1 Table.…”

Section: Resultsmentioning

confidence: 99%

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Accuracy of serological tests for diagnosis of chronic pulmonary aspergillosis: a systematic review and meta-analysis

Chaves

Oliveira

Venturini

et al. 2019

Preprint

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22Chronic pulmonary aspergillosis (CPA) is a disease that benefits from cavities as after-effects of 23 tuberculosis, presenting a high mortality rate. Serological tests like double agar gel 24 immunodiffusion test (DID) or the counterimmunoelectrophoresis (CIE) test have been routinely 25 used for CPA diagnosis in the absence of positive cultures; however, they have been replaced by 26 enzyme-linked immunoassay (ELISA), with a variety of methods. 27 This systematic review aims to compare the accuracy of the ELISA test with the reference test 28 (DID and/or CIE) in CPA diagnosis. It was conducted according to the Preferred Reporting Items 29 for Systematic Reviews and Meta-Analyzes (PRISMA). 30 The study was registered in PROSPERO under the registration number CRD42016046057. We 31 searched the electronic databases MEDLINE (PubMed), EMBASE (Elsevier), LILACS (VHL), 32 Cochrane library, and ISI Web of Science. Gray literature was researched in Google Scholars and 33 conference abstracts. We included articles with patients or serum samples from CPA patients who 34 underwent two serological tests: ELISA (index test) and IDD and/or CIE (reference test), using 35 the accuracy of the tests as a result. Original articles were considered without a restriction of date 36 or language. The pooled sensitivity, specificity, and summary receiver operating characteristic 37 curves were estimated. 38 We included 13 studies in the review, but only four studies were included in the meta-analysis. 39 The pooled sensitivities and specificities were 0.93 and 0.97 for the ELISA test. For the reference 40 test (DID and/or CIE), these values were 0.64 and 0.99. Analyses of summary receiver operating 41 characteristic curves yielded 0.99 for ELISA and 0.99 for the reference test (DID and/or CIE). Our 46 Chronic pulmonary aspergillosis (CPA) is a slow and progressive lung disease caused by 47 Aspergillus spp. that develops in preexisting cavities of patients with chronic respiratory diseases, 48 and pulmonary tuberculosis is its main predisposing factor, with a global prevalence estimated at 49 1.2 million cases [1].I Its prognosis is poor, with 38-85% mortality in five years [1,2]. 50CPA presents five clinical forms: 1. aspergillus nodule, 2. pulmonary simple aspergilloma, 51 3. chronic cavitary pulmonary aspergillosis (CCPA), also called complex aspergilloma, 4. chronic 52 fibrosing pulmonary aspergillosis (CFPA), and 5. subacute invasive pulmonary aspergillosis 53 (SAIA) [3]. Aspergilloma is present in only one-third of patients with CPA [1,4]. 54The diagnosis of CPA is based on suggestive images, preferably tomographic images (CT 55 scan), on evidence of microbiological infection by Aspergillus or on the presence of an immune 56 response to this agent, maintained for at least 3 months [3,5]. 57Serologic tests are indispensable for the diagnosis in the absence of positive cultures and 58 are considered the best noninvasive tests to diagnose this entity [6,7]. These tests may be over 90% 59 positive with preci...

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Role of Serological Tests in the Diagnosis of Mold Infections

Richardson

Page

2018

Curr Fungal Infect Rep

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Purpose of ReviewTo understand the role of antibody detection in the diagnosis of infections caused by filamentous fungi (molds). Rapid and accurate profiling of infection-causing fungal pathogens remains a significant challenge in modern health care. Classical fungal culture and serology continue to be relevant even though over the past few decades, antigen (biomarker) assays such as ELISA and lateral flow devices have been developed and validated.Recent FindingsThis article reviews the current antibody detection systems (serological tests) for the diagnosis of mold infections associated with pulmonary disease and introduces new developments. Classic and more recently developed serological techniques and their performance characteristics, including immunodiffusion, complement fixation, and ELISA.SummaryThe diseases covered are allergic bronchopulmonary aspergillosis, chronic pulmonary aspergillosis, invasive aspergillosis, mucormycosis, diseases caused by filamentous basidiomycetes, infection caused by Talaromyces marneffei and pythiosis. Serology remains a cornerstone for fungal diagnostic testing.

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Prospective Evaluation of a New Aspergillus IgG Enzyme Immunoassay Kit for Diagnosis of Chronic and Allergic Pulmonary Aspergillosis

Cited by 42 publications

References 28 publications

Role of Aspergillus fumigatus‐specific IgG in diagnosis and monitoring treatment response in allergic bronchopulmonary aspergillosis

Role of Aspergillus fumigatus‐specific IgG in diagnosis and monitoring treatment response in allergic bronchopulmonary aspergillosis

Accuracy of serological tests for diagnosis of chronic pulmonary aspergillosis: a systematic review and meta-analysis

Role of Serological Tests in the Diagnosis of Mold Infections

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