Evaluation of the Argyrophilic Nucleolar Organizer Region, Nuclear DNA Content and Mean Nuclear Area in Transitional Cell Carcinoma of Bladder Using a Quantitative Image Analyzer

Two-dimensional agnor evaluation as a prognostic variable in urinary bladder carcinoma: A different approach via total agnor area/nucleus area per cell

“…According to the data, we agreed with other researchers that the mean TAN/N per cell is not a useful tool for neither diagnostic nor prognostic purposes in TCC of urinary bladder (Iwata et al, 1995;Korneyev et al, 2000), while some previous data has not been consistent (Shiina et al, 1996;Pich et al, 2000). In contrast, by evaluating the TAA/NA ratio, and using our new software, we distinguished all groups from each other with high significance via mean TAA/NA proportion per cell.…”

“…The evaluation of cell proliferative activity is an important adjunct to the histological classification of tumors (Shiina et al, 1996). The AgNOR technique allows visualization of a set of argyrophilic nonhistone proteins (AgNORs) localized in the nucleolar organizer region (NOR) by the level of light microscopy.…”

“…www.elsevier.com/locate/micron Micron 38 (2007) [674][675][676][677][678][679] Although the diagnostic utility of the AgNOR technique is limited because of the overlap of AgNOR values between benign and malignant neoplasms. Studies on this histochemical method have shown the prognostic value of AgNOR amount to be an independent variable in predicting the recurrences and/or the survival in various kinds of malignancies (Shiina et al, 1996;Pich et al, 2000). Many different prognostic markers for optimal management of tumor biological potential in bladder cancer have been researched intensively and a large number of biomarkers are being used to diagnose bladder cancer and to determine therapeutic targets.…”

“…However, these markers are limited as indicators of prognosis (Stein et al, 1998;Han and Shoenberg, 2000). Although the AgNOR amount is a direct parameter of the cells proliferative activity in many cancer types (Shiina et al, 1996), in bladder cancer the correlation between AgNOR amount (number or area) and tumor behavior remains controversial (Tomobe et al, 1999;Korneyev et al, 2000;Han and Shoenberg, 2000;Pich et al, 2000). Therefore, we have suggested that the main problem may be in the method(s) of measuring AgNOR or means of evaluating the data, which are obtained by counting or area measuring.…”

Improved objectivity of grading of TA,1 transitional cell carcinomas of the urinary bladder by quantitative nuclear and proliferation related features

“…Mean nuclear area is easier to assess because standard histological sections can be used. The mean profile area of the 10 largest nuclei (MNA-10) found in a histological tumour section [8][9][10][11] has proved to be valuable as an independent marker for grade and prognosis. 12 In the study of Blomjous et al, 12 DNA diploid or DNA aneuploid cases (measured by flow cytometry) had nearly the same survival as MNA-10 low (< 95 µm 2 ) and MNA-10 high (> 95 µm 2 ) T A,1 tumours, respectively.…”