Assessment of cell proliferation by AgNOR scores and Ki-67 labeling indices and a comparison with potential doubling times

Dong, Hangying; Bertler, Carey; Schneider, Eric B.; Ritter, Mark A.

doi:10.1002/(sici)1097-0320(19970801)28:4<280::aid-cyto2>3.0.co;2-g

Cited by 20 publications

(17 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At high concentrations of tamoxifen, human breast tumour cells are killed (Katzenellenbogen et al, 1984). When MCF-7 human breast tumour cells were treated in our study with a cytostatic concentration of tamoxifen, the sigma-2 receptor density was reduced to the same extent (Table 4) as the Ki-67-labelling index, AgNOR scores and IdU-labelling index reported by Dong et al (1997). Taken together, these data suggest that the sigma-2 receptor density will likely reflect the proliferative status of human tumours before, during and after treatment with anticancer agents.…”

Section: Discussionsupporting

confidence: 67%

“…However, there was a reduction in the number of sigma-2 receptors per cell after 3 or 6 days of treatment with 1 nM tamoxifen ( Table 4) receptor density was 1.52 ± 0.41 after a 3-day exposure to 1 nM tamoxifen, and 2.23 ± 0.41 after a 6-day exposure to 1 nM tamoxifen. These values compare favourably with the reduction in the Ki-67 labelling index (1.24), AgNOR scores (1.32) and IdU-labelling index (2.36) reported by Dong et al (1997) for oestrogen-responsive MCF-7 cells whose growth had been reduced by 50% after exposure to 0.1-1.0 µM 4-hydroxytamoxifen.…”

Section: Effect Of Cytotoxic Concentrations Of Tamoxifen On the Expresupporting

confidence: 73%

“…the Ki-67 labelling index, AgNOR scores, and IdU-labelling index recently reported for these MCF-7 cells treated with 4-hydroxytamoxifen under identical conditions (Dong et al, 1997).…”

Section: Experimental Designmentioning

confidence: 64%

“…Similar BrdU labelling experiments were not performed for the MCF-7 cells because the in vitro growth conditions were identical to those where the proliferation kinetics had recently been determined by flow cytometrically measuring the Ki-67 and idoxuridine (IdU) labelling index (Dong et al, 1997).…”

Section: Cell Proliferation Analysismentioning

confidence: 99%

See 3 more Smart Citations

Effect of ploidy, recruitment, environmental factors, and tamoxifen treatment on the expression of sigma-2 receptors in proliferating and quiescent tumour cells

et al. 1999

View full text Add to dashboard Cite

Summary Recently, we demonstrated that sigma-2 receptors may have the potential to be a biomarker of tumour cell proliferation (Mach et al (1997) Cancer Res 57: 156-161). If sigma-2 receptors were a biomarker of tumour cell proliferation, they would be amenable to detection by non-invasive imaging procedures, thus eliminating many of the problems associated with the flow cytometric measures of tumour cell proliferation presently used in the clinic. To be a good biomarker of tumour cell proliferation, the expression of sigma-2 receptors must be essentially independent of many of the biological, physiological, and/or environmental properties that are found in solid tumours. In the investigation reported here, the mouse mammary adenocarcinoma lines, 66 (diploid) and 67 (aneuploid), 9L rat brain tumour cells, and MCF-7 human breast tumour cells were used to study the extent and kinetics of expression of sigma-2 receptors in proliferative (P) and quiescent (Q) tumour cells as a function of species, cell type, ploidy, pH, nutrient depletion, metabolic state, recruitment from the Q-cell compartment to the P-cell compartment, and treatment with tamoxifen. In these experiments, the expression of sigma-2 receptors solely reflected the proliferative status of the tumour cells. None of the biological, physiological, or environmental properties that were investigated had a measurable effect on the expression of sigma-2 receptors in these model systems. Consequently, these data suggest that the proliferative status of tumours and normal tissues can be non-invasively assessed using radiolabelled ligands that selectively bind sigma-2 receptors.

show abstract

Section: Discussionsupporting

confidence: 67%

Section: Effect Of Cytotoxic Concentrations Of Tamoxifen On the Expresupporting

confidence: 73%

“…the Ki-67 labelling index, AgNOR scores, and IdU-labelling index recently reported for these MCF-7 cells treated with 4-hydroxytamoxifen under identical conditions (Dong et al, 1997).…”

Section: Experimental Designmentioning

confidence: 64%

Section: Cell Proliferation Analysismentioning

confidence: 99%

See 2 more Smart Citations

Effect of ploidy, recruitment, environmental factors, and tamoxifen treatment on the expression of sigma-2 receptors in proliferating and quiescent tumour cells

et al. 1999

View full text Add to dashboard Cite

show abstract

“…Ki-67 antigen is expressed in proliferating cells and is therefore present in more than 50% of cells of a subconfluent cell culture (Sasaki et al 1989;Benbrook et al 1995;Dong et al 1997). For development of sensitive p53 immunostaining, we used (a) HPV-containing cervical cancer cell lines expressing low levels of wild-type p53 due to degradation by HPV E6 antigen (Scheffner et al 1991;Wrede et al 1991;Liang et al 1993) and (b) Molt-4 cells expressing very low levels of mutant p53 (Chow et al 1993).…”

Section: Microscopic Evaluationmentioning

confidence: 99%

AgarCyto: A Novel Cell-processing Method for Multiple Molecular Diagnostic Analyses of the Uterine Cervix

Kerstens¹,

Robben²,

Poddighe³

et al. 2000

J Histochem Cytochem.

View full text Add to dashboard Cite

S U M M A R YIn diagnostic cytology, it has been advocated that molecular techniques will improve cytopathological diagnosis and may predict clinical course. Ancillary molecular techniques, however, can be applied only if a sufficient number of preparations are made from a single cell sample. We have developed the AgarCyto cell block procedure for multiple molecular diagnostic analyses on a single scraping from the uterine cervix. The optimized protocol includes primary fixation and transport in ethanol/carbowax, secondary fixation in Unifix, and embedding in 2% agarose and then in paraffin according to a standard protocol for biopsies. More than 20 microscopic specimens were produced from a single AgarCyto cell block, and standard laboratory protocols have been successfully applied for H&E staining, immunohistochemistry for Ki-67 and p53, and in situ hybridization for the centromere of human chromosome 1 and human papilloma virus Type 16. In addition, single AgarCyto sections yielded sufficient input DNA for specific HPV detection and typing by LiPA-PCR, and the protocol includes an option for DNA image cytometry. The AgarCyto cell block protocol is an excellent tool for inventory studies of diagnostic and potentially prognostic molecular markers of cervical cancer.

show abstract

Relationship between morphometric analysis of nucleolar organizer regions and cell proliferation in acute leukemias

1998

View full text Add to dashboard Cite

Silver‐stained nucleolar organizer regions in interphase cells have been widely studied as a cell kinetic parameter in different tissues. Many different approaches have been used for this evaluation, often leading to conflicting results. The aim of our study was to analyze several commonly used AgNOR parameters in bone marrow smears of 32 newly diagnosed cases of acute leukemia: number of clusters and dots, percentage of cells with one cluster (cells in a long G1 phase), total and percentage of silver‐stained area per nucleus, mean size of AgNORs, and the Rüschoff index. These parameters were compared with the in vitro incorporation of bromodeoxyuridine (BrdU). A significant correlation was found between the BrdU index and the mean number of clusters (r = 0.60), dots (r = 0.60), percentage of cells with one cluster (r = ‐0.63), as well as with the mean size of AgNORs (r = ‐0.63) and the Rüschoff index (r = 0.72). In a factor analysis, total AgNOR area, its percentage of nuclear area, the mean AgNOR size, and the nuclear area formed one factor (factor of area parameters), and clusters, dots, the percentage of cells with one cluster, and BrdU index made another factor (factor of proliferation parameters). The Rüschoff index participated equally in both factors. Based on these data, we postulate that the number of clusters and dots are related to the percentage of cells in S‐phase and the AgNOR area may be related to the cell duplication time, as has been reported in the literature. Mean size of AgNORs is smaller in highly proliferating cells. Probably the Rüschoff index is related to the percentage of cells in S‐phase as well as to the cell duplication time, and seems to be the best AgNOR parameter for the global evaluation of cell kinetics in acute leukemias. Cytometry 32:51–56, 1998. © 1998 Wiley‐Liss, Inc.

show abstract

Assessment of cell proliferation by AgNOR scores and Ki-67 labeling indices and a comparison with potential doubling times

Cited by 20 publications

References 31 publications

Effect of ploidy, recruitment, environmental factors, and tamoxifen treatment on the expression of sigma-2 receptors in proliferating and quiescent tumour cells

Effect of ploidy, recruitment, environmental factors, and tamoxifen treatment on the expression of sigma-2 receptors in proliferating and quiescent tumour cells

AgarCyto: A Novel Cell-processing Method for Multiple Molecular Diagnostic Analyses of the Uterine Cervix

Relationship between morphometric analysis of nucleolar organizer regions and cell proliferation in acute leukemias

Contact Info

Product

Resources

About