Evaluation of the Apoptotic changes induced by 5-Fluorouracil on the Lingual Mucosa and Salivary glands of male albino rats  (Histological, Histomorphometric and Immunohistochemical Study)

El-Mansy, Merhan N.; Hegazy, Enas

doi:10.21608/edj.2020.41503.1240

Cited by 4 publications

(7 citation statements)

References 22 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the current study, observed Wistar rats' tongues showed a morphologic structure similar to other rat lines (Elmansy & Hegazy, 2020; Kullaa‐Mikkonen et al, 1987; Picoli et al, 2006; Verli et al, 2008) as the WWCPS rat (Goździewska‐Harłajczuk et al, 2018) or the Sprague–Dawley rat (Costa et al, 2013; Reginato et al, 2014).…”

Section: Discussionsupporting

confidence: 69%

Morphological structure of rat tongue using light and scanning electron microscopy

Demirci

Kandil

Yüksel

et al. 2022

Microscopy Res & Technique

View full text Add to dashboard Cite

The rat is one of the most commonly used animals in biological research and experimental investigations in medicine. The ultrafine structural components of the epithelium differ depending on the anatomy of the animal and the papilla type. Animal adaptation to food types and environmental circumstances may also be linked to morphological diversity. In the current study, seven male Wistar rat tongues were investigated. For scanning electron microscope (SEM), two rat tongues were immersed in a 10% formalin solution and the other two rat tongues were immersed in a 2.5% glutaraldehyde solution. The tongues of three rats were fixed for regular histological evaluation using triple staining. The three primary components of the Wistar rat tongue are the apex, body and root. The apex had a rounded and bifurcated shape. Filiform papillae and gustatory papillae were easily identified on the dorsal side of the tongue. There were three forms of gustatory papillae; fungiform papillae, vallate papillae and foliate papillae. The purpose of this study was to expose the tongue morphology of the Wistar rat species, which is widely used in investigations. Also, we wanted to show that formalin fixation can be utilized for morphological research in SEM. Finally, the Wistar rat tongue was thoroughly investigated and compared to those of other species. K E Y W O R D S fixation technique, papillar morphology, rat tongue Highlights• This research offers detailed morphological characterization of Wistar rat tongue.• We demonstrated that formol fixation, which is frequently preferred in histological and histopathological studies, can be an alternative method for scanning electron microscope. | INTRODUCTIONIn the oral cavity, the tongue is the most studied organ and examined as three parts; apex, corpus and radix linguae (Akbari et al., 2018;Goździewska-Harłajczuk et al., 2018). There is a median groove on the apex of the rat tongue (Goździewska-Harłajczuk et al., 2018;Jackowiak & Godynicki, 2005). The ultrafine structural elements of the epithelium depend on the morphology of different mammals and have different types of the papilla. In addition to the differences among animal species, variability may also be related to animal adaptation to food types and environmental conditions (Erdo gan,

show abstract

Section: Discussionsupporting

confidence: 69%

Morphological structure of rat tongue using light and scanning electron microscopy

Demirci

Kandil

Yüksel

et al. 2022

Microscopy Res & Technique

View full text Add to dashboard Cite

show abstract

“…5-FU causes this state of imbalance. The majority of the chemotherapeutic agents also pose many challenges to the integrity of the salivary glands [4].…”

Section: Discussionmentioning

confidence: 99%

“…Regrettably, they have several harmful effects on both normal and malignant cells, which are injured through a variety of pathways associated with the stomatognathic system's key structures and functional activities [2,3]. One of these chemotherapeutic medicines is fluorouracil (5-FU), which is used to treat solid malignancies such as breast, GIT, ovarian, Pharmaceuticals 2022, 15, 232 2 of 14 and brain tumours [4]. 5-FU is a pyrimidine analogue that blocks the incorporation of thymidylate synthase into RNA.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Xanthine Oxidase Inhibitor, Febuxostat Is Effective against 5-Fluorouracil-Induced Parotid Salivary Gland Injury in Rats Via Inhibition of Oxidative Stress, Inflammation and Targeting TRPC1/CHOP Signalling Pathway

et al. 2022

View full text Add to dashboard Cite

The current research aimed to examine the ameliorative role of febuxostat (FEB), a highly potent xanthine oxidase inhibitor, against 5-fluorouracil (5-FU)-induced parotid salivary gland damage in rats, as FEB is a pleiotropic drug that has multiple pharmacological effects. A total of 32 Wistar adult male rats were randomly arranged into four groups. Group 1: the control group; given only the vehicle for 14 days, then given a saline i.p. injection from the 10th to the 14th day. Group 2: the FEB group; rats received FEB (10 mg/kg) once daily po for 14 days before receiving a saline i.p. injection from the 10th to the 14th day. Group 3: the 5-FU group; from the 10th to the 14th day, rats received an intraperitoneal injection of 5-FU (35 mg/kg/day). Group 4: the FEB/5-FU group; rats were pre-treated with FEB po for 14 days before receiving 5-FU i.p injections for five consecutive days from the 10th to the 14th day. Parotid gland damage was detected histologically and biochemically by the evaluation of oxidative stress markers (malondialdehyde (MDA) and nitric oxide levels (NOx)), oxidant defences (reduced glutathione (GSH) and superoxide dismutase (SOD)), inflammatory markers (tumour necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β)), and transient receptor potential canonical1 (TRCP1) and C/EBP homologous protein (CHOP). FEB pre-treatment reduced MDA, TNF-, and IL-1 while increasing SOD, GSH, and NOx. FEB also significantly increased TRPC1 and decreased CHOP in parotid gland tissue. In conclusion, FEB pre-treatment reduced 5-FU-induced parotid salivary gland damage not only through its powerful anti-inflammatory and antioxidant effects, but also through its effect on the TRPC1/CHOP signalling pathway.

show abstract

“…It can be also be related to the fact that chemotherapeutic drugs can induce reactive oxygen species production leading to increased lipid peroxidation and calcium ions influx which also mediates apoptosis [9]. The epithelial atrophy in the acinar and ductal cells detected in group 2 of our study, obviously observed in the ED lining cells, could also be related to the impaired capacity of cell renewal due to the inhibitory effect of 5-FU on DNA replication and cell mitosis [27].…”

Section: Discussionmentioning

confidence: 64%

Systemic versus local injection of bone marrow mesenchymal stem cells on 5-fluorouracil treated parotid glands of albino rats

et al. 2021

View full text Add to dashboard Cite

Objective: Head and neck cancer is the sixth leading cancer by incidence worldwide and eighth by death. Recent reports revealed that, not only radiotherapy but also chemotherapy may induce xerostomia. The aim of this study was to compare the possible regenerative effect of BMSCs through systemic and local injections. Material and Methods: 52 male Albino rats were randomly divided into 4 groups: Group 1: 10 rats received 0.5 ml of PBS by injection. Group 2: 14 rats received an intraperitoneal injection of 5-FU drug. Group 3: 14 rats were injected the same dose of 5-FU then received an intraglandular transplantation of BMSCs suspended in 0.5 ml of PBS at day 1 after 5-FU administration. Group 4: 14 rats were injected the same dose of 5-FU then received an intravenous injection of BMSCs suspended in 0.5 ml of PBS via the tail vein at day 1 after 5-FU administration. Results: Histological examination showed that group 2 showed features of severe degenerative changes which increased over time. Group 3 showed increasing amelioration in the ductal structure overtime. Group 4 also showed regenerated ductal elements however concerning apoptotic changes, immunohistochemistry results revealed improvement in both group 3 and 4 over group 2 with no statistical difference between groups 3 and 4. Conclusion: Histological and immunohistochemical features in group 3 and group 4 revealed similar amelioration in regenerative potentials. On the other hand, regenerative features of both experimental groups were statistically significant as compared independently to group 1. KEYWORDS 5-fluourouracil; anti-caspase-3; BMSCs; H&E; parotid salivary gland. RESUMOObjetivo: O carcinoma de cabeça e pescoço é o sexto câncer de maior incidência no mundo sendo a oitava causa de morte por cancer. Relatos recentes revelaram que não apenas a radioterapia, mas também a quimioterapia podem induzir xerostomia. O objetivo desse estudo foi comparar a possivel ação regenerative de BMSCs através de injeção local e sistêmica. Material e Métodos: 52 ratos Albino foram aleatoriamente alocados em 4 grupos: Grupo 1: 10 ratos que receberam 0.5 ml de injeção de PBS. Grupo 2: 14 ratos que receberam injeção intraperitoneal da droga 5-FU. Grupo 3: 14 ratos que foram injetados com a mesma dose de 5-FU e receberam transplante intraglandular de BMSCs ressuspendidas em 0.5mL de PBS no dia 1 após a administração do 5-FU. Grupo 4: 14 ratos que foram injetados com a mesma dose de 5-FU e receberam injeção intravenosa de BMSCs ressuspendidas em 0.5mL de PBS via veia caudal 1 dia após a administração de 5-FU. Resultados: O exame histológico demonstrou que o grupo 2 apresentou alterações degenerativas severas que se agravaram com o tempo. O Grupo 3 mostrou melhora da estrutura ductal ao longo do experimento. Group 4 também mostrou elementos ductais regenerados. Referente a alterações apoptóticas,análise imunohistoquimica mostrou melhora nos grupos 3 e 4 comparados ao grupo 2, sendo que os grupos 3 e 4 foram estatisticamente semelhantes. Conclusão: Análises histológicas e imun...

show abstract

Evaluation of the Apoptotic changes induced by 5-Fluorouracil on the Lingual Mucosa and Salivary glands of male albino rats (Histological, Histomorphometric and Immunohistochemical Study)

Cited by 4 publications

References 22 publications

Morphological structure of rat tongue using light and scanning electron microscopy

Morphological structure of rat tongue using light and scanning electron microscopy

Xanthine Oxidase Inhibitor, Febuxostat Is Effective against 5-Fluorouracil-Induced Parotid Salivary Gland Injury in Rats Via Inhibition of Oxidative Stress, Inflammation and Targeting TRPC1/CHOP Signalling Pathway

Systemic versus local injection of bone marrow mesenchymal stem cells on 5-fluorouracil treated parotid glands of albino rats

Contact Info

Product

Resources

About