Evaluation of the antioxidant effects of polysaccharides extracted from Lycium barbarum

Abstract

Lycium barbarum, a famous Chinese medicinal herb, has a long history of use as a traditional remedy for many diseases. Polysaccharides are the most important functional constituent in Lycium barbarum fruits. In the present study, the antioxidant activity of polysaccharides extracted from Lycium barbarum fruits growing in Xin Jiang, a northwest province of China, was evaluated. Six established in vitro methods, including superoxide radical (O 2 -) scavenging activity, reducing power, b-carotene linoleate model, inhibition of mice erythrocyte hemolysis mediated by peroxyl free radicals, 1,1-diphenyl-2 picrylhydrazyl (DPPH) radical-scavenging, and metal chelating activity were used in our evaluation. The polysaccharides showed considerable inhibitory activity in the b-carotene-linoleate model system in a concentrationdependent manner. Further, they exhibited moderate concentration-dependent inhibition of the DPPH radical. The multiple antioxidant activity of the polysaccharides was evidenced by significant reducing power, superoxide scavenging ability, inhibition of mice erythrocyte hemolysis mediated by peroxyl free radicals, and also ferrous ion chelating potency. The data obtained in the in vitro models clearly establish the antioxidant potency of the polysaccharides extracted from Lyceum barbarum fruits.

Synthesis, characterization, stability evaluation and release kinetics of fiber-encapsulated carotene nano-capsules

“…In case of DPPH radical scavenging activity, the activity was directly proportional to the concentration of the anti-oxidants. The DPPH radical scavenging method was based on the reduction in the methanol DPPH solution in the presence of a hydrogen donating antioxidant, due to the formation of the non-radical form DPPH-H (Li and Zhou, 2007). It was observed that the carotenoids were able to reduce and decolor 1,1-diphenyl-2-picrylhydrazyl, via their hydrogen donating ability both in encapsulated form and before encapsulation.…”

A study on four antioxidation effects of <i>Lycium Barbarum</i> polysaccharides <i>In vitro</i>

“…We referred to the methods in the literature (Zheng et al, 2010;Li et al, 2006b) and made minor modifications. Precisely, DPPH powder was weighed, dissolved with methanol, and prepared into 6mmol/L solution for later use.…”

Untitled

“…Pharmacological investigations on the antioxidant effect of L. barbarum mainly attributed this capacity to L. barbarum polysaccharides (LBP) (Li et al, 2007a;Li & Zhou, 2007), but also to other components such as flavonoids or carotenoids (Le et al, 2007;Jiang, 2014). The mechanism of action of both classes of compounds includes metal reducing and chelating capabilities, as well as the ability to clean free radicals (Li & Zhou, 2007).…”

“…The mechanism of action of both classes of compounds includes metal reducing and chelating capabilities, as well as the ability to clean free radicals (Li & Zhou, 2007).…”