Synthesis, characterization, stability evaluation and release kinetics of fiber-encapsulated carotene nano-capsules

Abstract

SUMMARY:In the present work, carotenoids were isolated (1.2%) from crude palm oil and encapsulated with isabgol fiber (Psyllium husk). The efficiency of encapsulation was 82.23±1.42%. The morphology of the capsules showed rough surface texture with minimal pores. The amorphous natures of the nano-capsules was obvious from X-ray diffraction patterns. DSC studies showed high thermal stability of the nano-capsules between 20-120 °C. In vitro release studies revealed that controlled release from the nano-capsules could be achieved using isabgol fiber as encapsulant. However it was observed that the nano-capsules followed a non-Fickian diffusion pattern. Good DPPH-radical scavenging and metal-chelation activities were observed for encapsulated carotenoids. Shelf-life studies showed that the nano-capsules gradually degraded at 97% relative humidity, as the moisture-induced rancidity was evidently not extensive.Keywords: Carotenoids; Isabgol fiber; Nano-capsules; Release; Release kinetics; Stability RESUMEN: Síntesis, caracterización, evaluación de la estabilidad y cinética de liberación de caroteno encapsulado en nanocápsulas de fibra. En el presente trabajo los carotenos fueron aislados (1,2%) a partir de aceite de palma crudo y encapsulados con fibra de isabgol (cáscara de psyllium). La eficiencia de encapsulación fue 82,23±1,42 %. La morfología de las cápsulas mostró una textura áspera de la superficie con mínimos poros. La naturaleza amorfa de las nanocápsulas fue evidenciada a partir de patrones de difracción de rayos X. Los estudios de DSC mostraron una alta estabilidad térmica de las nanocápsulas entre 20-120 °C. En los estudios de liberación in vitro se vió que la liberación controlada de las nanocápsulas se podría lograr mediante fibra isabgol como encapsulante. Sin embargo se observó que las nanocápsulas siguieron un patrón de difusión noFickian. Se observaron buenas actividades DPPH captadoras de radicales y actividades de quelación de metal para carotenoides encapsulados. Los estudios sobre la vida útil mostraron que las nanocápsulas se degradan gradualmente con una humedad relativa del 97%, y aunque la humedad indujo el enranciamiento, este hecho no se puede generalizar.PALABRAS CLAVE: Carotenoides; Cinética de liberación; Estabilidad; Fibra isabgol; Liberación; Nanocápsulas Citation/Cómo citar este artículo: Sen Gupta S, Ghosh M. 2015. Synthesis, characterization, stability evaluation and release kinetics of fiber-encapsulated carotene nano-capsules. Grasas Aceites 66 (4): e104. doi: http://dx.doi.

Bioavailability and Bioactivity of Encapsulated Phenolics and Carotenoids Isolated from Red Pepper Waste

“…Incubation conditions (temperature, light exposure, and long duration of the assay) probably have a large impact on the oxidation and isomerization of carotenoids, due to their sensitivity toward exposed factors, and consequently on the degradation of these molecules. Similar carotenoid release behavior was reported by Sen Gupta and Ghosh [37] for fiber-encapsulated carotene nano-capsules.…”

Physicochemical properties, antioxidant activities and in vitro sustained release behaviour of co‐encapsulated liposomes as vehicle for vitamin E and β‐carotene

“…In a previous report on co‐encapsulated liposomes of vitamin C and βC, the free radical scavenging rates of L‐VC‐βC, including DPPH, ABTS and OH radicals, were significantly higher than that of L‐βC, but had no significant difference from that of L‐VC 9 . In some other reports of βC loaded nanocarriers, for example, βC loaded nanostructured lipid carrier (NLC) from palm stearin and palm olein, fibre‐encapsulated carotene nanocapsules, only slight increases of radical scavenging rates were reported compared with unloaded βC 48,49 . Taken together, co‐encapsulation of VE and βC could improve the radical scavenging activities significantly, especially the combination at 5:3 with the highest antioxidant activities.…”

“…9 In some other reports of ⊎C loaded nanocarriers, for example, ⊎C loaded nanostructured lipid carrier (NLC) from palm stearin and palm olein, fibre-encapsulated carotene nanocapsules, only slight increases of radical scavenging rates were reported compared with unloaded ⊎C. 48,49 Taken together, co-encapsulation of VE and ⊎C could improve the radical scavenging activities significantly, especially the combination at 5:3 with the highest antioxidant activities.…”